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EP0212951A2
Expired / Lapsed
EP
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Labelled nucleic acids
Assignee: BP Corp North America Inc
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Filing: 1986-08-15
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Granted: —
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Expires: 2006-08-15
Abstract
Abstract Polynucleotides are non-isotopically labelled with acridine esters or luminescent lanthanides.
Inventors
- 👤 Graham A. Mock
- 👤 Michael J. Powell
- 👤 Michael J. Septak
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 22:44
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
25 words
A method of synthesizing nonisotopically a labelled nucleotide or polynucleotide comprising: incorporating an acridine ester or a luminescent lanthanide into said nucleotide or said polynucleotide.
Claim 2
Independent Claim
25 words
A method of synthesizing nonisotopically a labelled nucleotide or polynucleotide comprising: incorporating an acridine ester or a luminescent lanthanide into said nucleotide or said polynucleotide.
Claim 3
Dependent Claim
54 words
A method as claimea in claim 1 wherein said acridine ester has the structure: where T is chosen from a saturated aliphatic chain, an unsaturated aliphatic chain, an aliphatic chain containing a sulfite group, an aliphatic chain containing an amide group, an aliphatic chain containing two hydroxyl groups and A is a leaving group.
Claim 4
Dependent Claim
101 words
A method as claimed in claim 1 wherein said luminescent lanthanide is chelated with a compound of formula (1) or (2): where Z is chosen from (a) N = C = S, where W is a halogen, (d) N 3 , where V is an alkyl or phenyl group, and 1 ≤ n ≤ 10, where 1 ≤ n ≤ 10, and (g) where 1 L n ≤ 10; and where Y is chosen from (a) and (b) where each n, m and p, independently, is from 2-4, inclusive; where 2 ≤ n ≤ 10 and 2 ≤ m ≤ 4.
Claim 5
Dependent Claim
13 words
A method according to claim 2 wherein said A moiety has the structure:
Claim 6
Dependent Claim
14 words
A nucleotide or polynucleotide labelled with an acridine ester or a luminescent lanthanide .
Claim 7
Dependent Claim
14 words
A nucleotide or polynucleotide labelled with an acridine ester or a luminescent lanthanide .
Claim 8
Dependent Claim
17 words
A nucleotide or polynucleotide as claimed in claim 5 wherein said lanthanide is europium, terbium or samarium.
Claim 9
Dependent Claim
13 words
A nucleotide or polynucleotide labeled with a group able to chelate a lanthanide.
Claim 10
Dependent Claim
13 words
A nucleotide or polynucleotide labeled with a group able to chelate a lanthanide.
Claim 11
Dependent Claim
14 words
A nucleotide or polynucleotide as claimed in claim 7, wherein the lanthanide is europium.
Claim 12
Dependent Claim
37 words
A nucleotide or polynucleotide as claimed in claim 7 or 8, wherein said chelating group is or or or where each n, m and p, independently, is from 2-4, inclusive; or where 2 L m ≤ 4.
Claim 13
Dependent Claim
26 words
A nucleotide or polynucleotide as claimed in claim 6 wherein said acridine ester is chemically linked to an amine group on said nucleotide or said polynucleotide.
Claim 14
Dependent Claim
44 words
A nucleotide or polynucleotide as claimed in claim 10 wherein said amine group is introduced onto said nucleotide or said polynucleotide by chemically or enzymically attaching to said nucleotide or said polynucleotide a further nucleotide or a further polynucleotide which comprises said amine group.
Claim 15
Dependent Claim
22 words
A nucleotide or polynucleotide as claimed in claim 9 wherein said nucleotide or said polynucleotide is chelated to europium, turbium or samarium.
Claim 16
Independent Claim
39 words
A method as claimed in any one of claims 1 to 4, wherein said method comprises chemically modifying said nucleotide or said polynucleotide and linking said acridine ester or luminescent lanthanide to said modified nucleotide or said modified polynucleotide.
Claim 17
Dependent Claim
20 words
A method as claimed in claim 13, wherein said modifying comprises alkylation of a base of said nucleotide or polynucleotide.
References Cited (Backward Citations)
No citation records indexed.
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