US4818681A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fast and specific immobilization of nucleic acids to solid supports
Assignee: MILES INC. A CORP. OF INDIANA
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Filing: 1985-02-22
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Granted: 1989-04-04
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Expires: 2005-02-22
Abstract
Abstract A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a mono- or oligonucleotide to the nucleoside phosphate through its phosphate moiety, in at least one step enzymatically lengthening the mono- or oligonucleotide, cleaving the resultant oligonucleotide from the solid support-nucleoside phosphate at the phosphate moiety of the nucleoside, and separating the oligonucleotide. After cleaving and separating the solid support-nucleoside phosphate is recycled for further coupling. Advantageously the solid support-nucleoside phosphate is phosphorylated between separation and recycling.
Inventors
- 👤 Nanibhushan Dattagupta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 14:50
CPC Classifications
C; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
49 words
A process for immobilization of a nucleic acid comprising (a) linking a nucleoside phosphate to a solid support through the nitrogen heterocyclic moiety of the nucleoside phosphate and (b) enzymatically covalently coupling a nucleic acid to the resultant immobilized nucleoside phosphate from step (a) via a phosphate or linkage.
Claim 2
Dependent Claim
49 words
A process for immobilization of a nucleic acid comprising (a) linking a nucleoside phosphate to a solid support through the nitrogen heterocyclic moiety of the nucleoside phosphate and (b) enzymatically covalently coupling a nucleic acid to the resultant immobilized nucleoside phosphate from step (a) via a phosphate or linkage.
Claim 3
Dependent Claim
18 words
A process according to claim 1, wherein the nucleic acid is a sickle cell anemia nucleic acid probe.
Claim 4
Dependent Claim
25 words
A process according to claim 1, wherein the enzymatic coupling is conducted in the presence of an enzyme, wherein the enzyme is terminal deoxynucleotidyl transferase.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-03 | 1992-10-03 | 1993-04-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-03 | 1996-10-03 | 1997-04-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-03 | 2000-10-03 | 2001-04-04 | $7700.00 | Window Closed / Lapsed |