Patent US2060850A successfully refreshed from Google Patents.
US5518907A
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Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta-hydroxybutyrate biosynthetic pathway
Assignee: James Madison University
•
Filing: 1994-05-31
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Granted: 1996-05-21
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Expires: 2014-05-31
Abstract
Abstract Genes coding for poly-beta-hydroxybutyrate were removed from Alcaligenes eutrophus H16 and cloned into Escherichia coli. Some of the clones produced PHB to 90% of the cell weight.
Inventors
- 👤 Douglas E. Dennis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 01:17
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/62; C12P7/625; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
68 words
An Escherichia coli bacterial host transformed by a vector containing a DNA sequence coding for a poly-beta-hydroxybutyrate biosynthetic pathway obtained from Alcaligenes eutrophus; wherein the DNA sequence is cloned into a multicopy vector; said Escherichia coil bacterial host being capable of expressing said biosynthetic pathway by producing poly-beta-hydroxybutyrate in recoverable quantities of at least about 54% of the bacterial cell dry weight of the Escherichia coli bacterial host.
Claim 2
Dependent Claim
68 words
An Escherichia coli bacterial host transformed by a vector containing a DNA sequence coding for a poly-beta-hydroxybutyrate biosynthetic pathway obtained from Alcaligenes eutrophus; wherein the DNA sequence is cloned into a multicopy vector; said Escherichia coil bacterial host being capable of expressing said biosynthetic pathway by producing poly-beta-hydroxybutyrate in recoverable quantities of at least about 54% of the bacterial cell dry weight of the Escherichia coli bacterial host.
Claim 3
Dependent Claim
20 words
The Escherichia coil bacterial host as recited in claim 1, where the vector containing the DNA sequence is plasmid pSB20.
Claim 4
Dependent Claim
19 words
The Escherichia coli bacterial host of claim 1, wherein the poly-beta-hydroxybutyrate is recovered after about a 24 hour period.
Claim 5
Dependent Claim
33 words
An Escherichia coil bacterial host as recited in claim 1 wherein the vector is a plasmid containing a nucleic acid fragment containing said biosynthetic pathway, said fragment being approximately 5.5 kilobases in length.
Claim 6
Dependent Claim
17 words
An Escherichia coli bacterial host as recited in claim 1 bearing the following deposit number: ATCC 68006.
Claim 7
Independent Claim
77 words
A method of making poly-beta-hydroxybutyrate comprising the steps of: culturing an Escherichia coil bacterial host transformed by a vector containing a DNA sequence coding for a poly-beta-hydroxybutyrate biosynthetic pathway original from Alcaligenes eutrophus, wherein the DNA sequence is cloned into a multicopy vector; obtaining expression of said poly-beta-hydroxybutyrate biosynthetic pathway in said Escherichia coli bacterial host; and recovering said poly-beta-hydroxybutyrate in quantities of about 54% of the bacterial cell dry weight of the Escherichia coli bacterial host.
Claim 8
Independent Claim
77 words
A method of making poly-beta-hydroxybutyrate comprising the steps of: culturing an Escherichia coil bacterial host transformed by a vector containing a DNA sequence coding for a poly-beta-hydroxybutyrate biosynthetic pathway original from Alcaligenes eutrophus, wherein the DNA sequence is cloned into a multicopy vector; obtaining expression of said poly-beta-hydroxybutyrate biosynthetic pathway in said Escherichia coli bacterial host; and recovering said poly-beta-hydroxybutyrate in quantities of about 54% of the bacterial cell dry weight of the Escherichia coli bacterial host.
Claim 9
Dependent Claim
13 words
The method as recited in claim 6, wherein the vector is plasmid pSB20.
Claim 10
Dependent Claim
17 words
The method as recited in claim 6, wherein poly-beta-hydroxybutyrate is recovered after about a 24 hour period.
References Cited (Backward Citations)
US2811511A
Cited
US3021309A
Cited
US3036959A
Cited
US3044942A
Cited
US3107172A
Cited
US3121669A
Cited
US3293225A
Cited
US3314801A
Cited
US3275610A
Cited
US3406114A
Cited
US3579549A
Cited
US3553081A
Cited
US3632570A
Cited
US3624047A
Cited
US3806495A
Cited
US3923782A
Cited
US4237224A
Cited
US4101533A
Cited
US4140741A
Cited
US4306026A
Cited
US4211846A
Cited
US4138291A
Cited
US4326035A
Cited
US4499189A
Cited
US4495287A
Cited
US4487835A
Cited
US4394447A
Cited
US4310684A
Cited
US4336334A
Cited
US4324907A
Cited
US4329448A
Cited
US4385026A
Cited
US4391766A
Cited
US4360488A
Cited
US4324880A
Cited
US4337181A
Cited
US4427614A
Cited
US4508823A
Cited
US4365088A
Cited
US4358583A
Cited
US4433053A
Cited
GB2089823B
Cited
US4396763A
Cited
US4529797A
Cited
US4477654A
Cited
US4503155A
Cited
US4477655A
Cited
US4567140A
Cited
GB2120671B
Cited
US5110852A
Cited
US4599311A
Cited
US4537738A
Cited
US4760022A
Cited
US4806471A
Cited
US4491575A
Cited
US4562245A
Cited
US4575551A
Cited
US4631259A
Cited
US4752580A
Cited
US4758356A
Cited
US4626504A
Cited
US4638059A
Cited
US4620999A
Cited
US4910145A
Cited
US4957861A
Cited
US4786598A
Cited
US4711848A
Cited
US4952496A
Cited
US4705604A
Cited
US4603070A
Cited
US4743453A
Cited
US4992540A
Cited
US5082936A
Cited
US5037972A
Cited
US4713449A
Cited
US4647657A
Cited
GB2180540A
Cited
US4806482A
Cited
US4948733A
Cited
US5091376A
Cited
US5008108A
Cited
US5135859A
Cited
US4960866A
Cited
US5032512A
Cited
US4902516A
Cited
US4997909A
Cited
US4900299A
Cited
US4826945A
Cited
US4965197A
Cited
US5229279A
Cited
US4876331A
Cited
US5028703A
Cited
US4968611A
Cited
US5059536A
Cited
US5096819A
Cited
US4950749A
Cited
US5107016A
Cited
US5004664A
Cited
US5110980A
Cited
US5126255A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-05-21 | 1999-11-20 | 2000-05-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-05-21 | 2003-11-20 | 2004-05-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-05-21 | 2007-11-20 | 2008-05-21 | $7700.00 | Window Closed / Lapsed |