US4499189A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Bacteria carrying plasmid having temperature dependent plasmid copy number
Assignee: BENZON PHARMA A DANISH Ltd Co
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Filing: 1980-12-08
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Granted: 1985-02-12
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Expires: 2000-12-08
Abstract
Abstract Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperature and a much higher or totally uncontrolled copy number at a different temperature. The plasmid may be prepared by recombinant DNA technique using a cloning vector showing the temperature dependent plasmid copy number pattern.
Inventors
- 👤 Bernt E. Uhlin
- 👤 Kurt Nordstrom
- 👤 Soeren Molin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 12:26
CPC Classifications
C; C09; C09K; C09K8/00; C09K8/02; C09K8/04; C09K8/26; C09K23/00; C09K8/28; C09K8/32; C09K8/36; C09K8/42; C09K8/424; C09K8/58; C09K8/584; C09K8/60; C09K8/601; C10; C10L; C10L1/00; C10L1/32; C10L1/328; C12; C12N; C12N15/00; C12N15/01; C12N15/09; C12N15/63; C12N15/67; C12N15/69; Y; Y10; Y10S; Y10S435/00; Y10S435/82
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
51 words
A bacterium carrying a recombined DNA plasmid showing a controlled constant plasmid copy number when the bacterium is cultivated at one temperature, and showing, when the bacterium is grown at a different temperature, an altered plasmid copy number control allowing an at least 20 times higher or totally uncontrolled copy number.
Claim 2
Dependent Claim
51 words
A bacterium carrying a recombined DNA plasmid showing a controlled constant plasmid copy number when the bacterium is cultivated at one temperature, and showing, when the bacterium is grown at a different temperature, an altered plasmid copy number control allowing an at least 20 times higher or totally uncontrolled copy number.
Claim 3
Dependent Claim
53 words
A bacterium as claimed in claim 1 which is a mesophilic bacterium and wherein the plasmid shows a controlled constant plasmid copy number at temperatures up to 32° C., and an altered plasmid copy number control allowing an at least 20 times higher or totally uncontrolled copy number at temperatures above 36° C.
Claim 4
Dependent Claim
12 words
A bacterium as claimed in claim 2 which is an Escherichia coli.
Claim 5
Dependent Claim
17 words
A bacterium as claimed in any of claims 1 to 3, wherein the plasmid is a mini-plasmid.
Claim 6
Dependent Claim
15 words
A bacterium as claimed in claim 1, wherein the plasmid is a derivative of R1.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-02-12 | 1988-08-13 | 1989-02-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-02-12 | 1992-08-13 | 1993-02-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-02-12 | 1996-08-13 | 1997-02-12 | $7700.00 | Window Closed / Lapsed |