US4394447A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of high-pyruvate xanthan gum on synthetic medium
Assignee: United States Department of Agriculture
•
Filing: 1978-05-23
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Granted: 1983-07-19
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Expires: 1998-05-23
Abstract
Abstract A high-pyruvate xanthan gum, substantially free of insolubles and undesirable coloration, is produced by fermentation of Xanthomonas campestris on a carbohydrate-containing nutrient medium having (NH4)2HPO4 at a level of at least 0.15% as the primary nitrogen source, and having a total phosphate level of at least about 0.25%.
Inventors
- 👤 Martin C. Cadmus
- 👤 Clarence A. Knutson, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 10:44
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/04; C12P19/06; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/91
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
111 words
In a process for producing polysaccharides by fermentation of a carbohydrate-containing nutrient medium with the bacterium Xanthomonas campestris, wherein said bacterium is cultivated on said medium under conditions suitable for its growth, the improvement comprising: a. providing (NH 4 ) 2 HPO 4 as the primary nitrogen source in said nutritent medium at a level of at least 0.15 g./100 ml. of medium; b. providing a total phosphate level in said nutrient medium of at least 0.25 g./100 ml. of medium; and c. recovering from said fermentation medium a polysaccharide isolated from residual solids in said medium wherein said polysaccharide has a pyruvic acid content of at least 3.3% by weight.
Claim 2
Dependent Claim
111 words
In a process for producing polysaccharides by fermentation of a carbohydrate-containing nutrient medium with the bacterium Xanthomonas campestris, wherein said bacterium is cultivated on said medium under conditions suitable for its growth, the improvement comprising: a. providing (NH 4 ) 2 HPO 4 as the primary nitrogen source in said nutritent medium at a level of at least 0.15 g./100 ml. of medium; b. providing a total phosphate level in said nutrient medium of at least 0.25 g./100 ml. of medium; and c. recovering from said fermentation medium a polysaccharide isolated from residual solids in said medium wherein said polysaccharide has a pyruvic acid content of at least 3.3% by weight.
Claim 3
Dependent Claim
26 words
The process as defined in claim 1 wherein said (NH 4 ) 2 HPO 4 is provided in an amount of 0.15-0.25 g./100 ml. of medium.
Claim 4
Dependent Claim
17 words
The process as defined in claim 1 wherein said nutrient medium comprises dipotassium phosphate or disodium phosphate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-18 | 1987-01-17 | 1987-07-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-18 | 1991-01-17 | 1991-07-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-18 | 1995-01-17 | 1995-07-19 | $7700.00 | Window Closed / Lapsed |