Atypically salt-responsive alkali-deacetylated polysaccharide produced by xanthomonas campestris

Claim 1

A FILM-FORMING COMPOSITION CONSISTING ESSENTIALLY OF A 1 PERCENT SOLUTION IN AQUEOUS GLYCEROL OF THE ALKALI DEACETYLATED DERIVATIVE OF THE NATIVE POLYSACCHARIDE B-1459, SAID NATIVE POLYSACCHARIDE B-1459 HAVING BEEN PRECIPITATED BY THE ADDITION OF METHANOL AND POTASSIUM CHLORIDE TO PROVIDE RESPECTIVE CONCENTRATIONS OF 56 PERCENT AND 2 PERCENT THEREOF IN A CENTRIFUGED 72 TO 96 HOUR AEROBIC WHOLE CULTURE FERMENTATION OF XANTHOMONAS CAMPESTRIS NRRL B-1459 AND THEN ISOLATED, THE ALKALIDEACETYLATED DERIVATIVE OF SAID POLYSACCHARIDE B-1459 HAVING BEEN OBTAINED BY DISSOLVING UNDER NITROGEN, 0.67 PARTS OF THE ISOLATED NATIVE POLYSACCHARIDE B-1459 IN 100 PARTS OF AIR-FREE WATER CONTAINING 1.35 PARTS OF POTASSIUM CHLORIDE, VIGOROUSLY STIRRING IN 34 PARTS OF 0.20 N POTASSIUM HYDROXIDE SOLUTION, MAINTAINING THE MIXTURE AT 25* C. FOR 2 HOURS UNDER NITROGEN, ADJUSTING THE PH TO NEUTRALITY, PRECIPITATING THE DEACETYLATED POLYSACCHARIDE BY ADDING METHANOL TO PROVIDE A CONCENTRATION THEREOF OF 44 PERCENT BY WEIGHT, AND ISOLATING THE DEACETYLATED POLYSACCHARIDE.