US4487835A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Plasmid having temperature dependent plasmid copy number
Assignee: Takeda Pharma AS
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Filing: 1980-12-08
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Granted: 1984-12-11
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Expires: 2000-12-08
Abstract
Abstract Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperature and a much higher or totally uncontrolled copy number at a different temperature. The plasmid may be prepared by recombinant DNA technique using a cloning vector showing the temperature dependent plasmid copy number pattern.
Inventors
- 👤 Bernt E. Uhlin
- 👤 Kurt Nordstr/o/ m
- 👤 Soeren Molin
- 👤 Petter Gustaffson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 14:23
CPC Classifications
C; C09; C09K; C09K8/00; C09K8/02; C09K8/04; C09K8/26; C09K23/00; C09K8/28; C09K8/32; C09K8/36; C09K8/42; C09K8/424; C09K8/58; C09K8/584; C09K8/60; C09K8/601; C10; C10L; C10L1/00; C10L1/32; C10L1/328; C12; C12N; C12N15/00; C12N15/01; C12N15/09; C12N15/63; C12N15/67; C12N15/69; Y; Y10; Y10S; Y10S435/00; Y10S435/82
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
55 words
A recombinant DNA plasmid showing a controlled constant plasmid copy number when host bacteria carrying the plasmid are cultivated at one temperature, and showing, when the host bacteria carrying the plasmid are grown at a different temperature, an altered plasmid copy number control allowing an at least 20 times higher or totally uncontrolled copy number.
Claim 2
Dependent Claim
55 words
A recombinant DNA plasmid showing a controlled constant plasmid copy number when host bacteria carrying the plasmid are cultivated at one temperature, and showing, when the host bacteria carrying the plasmid are grown at a different temperature, an altered plasmid copy number control allowing an at least 20 times higher or totally uncontrolled copy number.
Claim 3
Dependent Claim
44 words
A plasmid as claimed in claim 1 which shows an altered copy number control allowing an at least 20 times higher or totally uncontrolled copy number at a higher temperature than the temperature at which the plasmid shows a controlled constant plasmid copy number.
Claim 4
Dependent Claim
47 words
A plasmid as claimed in claim 2 showing a constant controlled plasmid copy number at a temperature up to about 32° C. and showing an altered copy number control allowing an at least 20 times higher or totally uncontrolled copy number at temperatures of about 36° C.
Claim 5
Dependent Claim
21 words
A plasmid as claimed in claim 1 and carrying a marker useful for identification and selection of cells carrying the plasmid.
Claim 6
Dependent Claim
21 words
A plasmid as claimed in claim 4 wherein the marker is a gene that mediates antibiotic resistance to the host bacterium.
Claim 7
Dependent Claim
15 words
A plasmid as claimed in claim 5 in which the antibiotic resistance is ampicillin resistance.
Claim 8
Dependent Claim
17 words
A plasmid as claimed in any of claims 1-3 and 4-6, wherein the plasmid is a mini-plasmid.
Claim 9
Dependent Claim
15 words
A plasmid as claimed in claim 1, wherein the plasmid is a derivative of R1.
Claim 10
Dependent Claim
61 words
A plasmid capable of receiving a fragment of DNA and showing a controlled constant plasmid copy number when host bacteria carrying the plasmid are cultivated at one temperature, and showing, when the host bacteria carrying the plasmid are grown at a different temperature, an altered plasmid copy number control allowing an at least 20 times higher or totally uncontrolled copy number.
Claim 11
Dependent Claim
61 words
A plasmid capable of receiving a fragment of DNA and showing a controlled constant plasmid copy number when host bacteria carrying the plasmid are cultivated at one temperature, and showing, when the host bacteria carrying the plasmid are grown at a different temperature, an altered plasmid copy number control allowing an at least 20 times higher or totally uncontrolled copy number.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-12-11 | 1988-06-11 | 1988-12-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-11 | 1992-06-11 | 1992-12-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-11 | 1996-06-11 | 1996-12-11 | $7700.00 | Window Closed / Lapsed |