US4599311A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Glycolytic promotersfor regulated protein expression: protease inhibitor
Assignee: Individual
•
Filing: 1982-08-13
•
Granted: 1986-07-08
•
Expires: 2002-08-13
Abstract
Abstract Promoters associated with expression of specific enzymes in the glycolytic pathway are used for expression of alien DNA, particularly yeast promoters known to provide high enzyme levels of enzymes in the glycolytic pathway are employed for expressing a mammalian protein, such as α 1 -antitrypsin. The promoters include promoters involved in expression of pyruvate kinase, triose phosphate isomerase, phosphoglucose isomerase, phosphoglycerate mutase, hexokinase 1, hexokinase 2, glucokinase, phosphofructokinase, and aldolase, as well as the glycolytic regulation gene. Particularly, the glycolytic regulation gene can be used in conjuction with promoters in the glycolytic pathway for regulated production of desired proteins.
Inventors
- 👤 Glenn H. Kawasaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 05:41
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/81; C07K14/8107; C07K14/811; C07K14/8121; C07K14/8125; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/30
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
60 words
A DNA expression vector capable of replication in Saccharomyces cerevisiae and containing a Saccharomyces cerevisiae promoter which regulates the transcription of a glycolytic protein, which protein is triose phosphate isomerase or pyruvate kinase, said Saccharomyces cerevisiae followed downstream by a gene, under the regulation of said promoter, expressing a protein other than the glycolytic protein normally regulated by such promoter.
Claim 2
Dependent Claim
60 words
A DNA expression vector capable of replication in Saccharomyces cerevisiae and containing a Saccharomyces cerevisiae promoter which regulates the transcription of a glycolytic protein, which protein is triose phosphate isomerase or pyruvate kinase, said Saccharomyces cerevisiae followed downstream by a gene, under the regulation of said promoter, expressing a protein other than the glycolytic protein normally regulated by such promoter.
Claim 3
Dependent Claim
17 words
A DNA expression vector according to claim 1, having a marker for selection in a Saccharomyces cerevisiae.
Claim 4
Dependent Claim
26 words
A DNA expression vector according to claim 1, having a gene expressing a foreign protein downstream from said promoter and under the regulation of said promoter.
Claim 5
Dependent Claim
28 words
A DNA expression vector according to claim 1, wherein said promoter is followed by a gene expressing protease inhibitor having substantially the same structure as α 1 -antitrypsin.
Claim 6
Dependent Claim
23 words
A DNA expression vector according to any of claims 1, 2, 3 or 4, wherein said promoter is the triose phosphate isomerase promoter.
Claim 7
Dependent Claim
24 words
A DNA expression vector according to claim 5, wherein a gene expressing an enzyme in a metabolic pathway of said Saccharomyces cerevisiae is present.
Claim 8
Dependent Claim
22 words
A DNA expression vector according to any of claims 1, 2, 3 or 4, wherein said promoter is the pyruvate kinase promoter.
Claim 9
Dependent Claim
24 words
A DNA expression vector according to claim 7, wherein a gene expressing an enzyme in a metabolic pathway of said Saccharomyces cerevisiae is present.
Claim 10
Dependent Claim
18 words
A Saccharomyces cerevisiae cell containing an extrachromosomal element according to any of claims 1, 2, 3 or 4.
Claim 11
Dependent Claim
26 words
A DNA expression vector comprising a Saccharomyces cerevisiae triose phosphate isomerase promoter followed by a gene expressing a foreign protein under the regulation of said promoter.
Claim 12
Dependent Claim
26 words
A DNA expression vector comprising a Saccharomyces cerevisiae triose phosphate isomerase promoter followed by a gene expressing a foreign protein under the regulation of said promoter.
Claim 13
Dependent Claim
19 words
A DNA expression vector according to claim 10, wherein said foreign protein is a protein having protease inhibition activity.
Claim 14
Dependent Claim
24 words
A DNA expression vector according to claim 11, wherein the major sequence of said protein is the same sequence as human α 1 -antitrypsin.
Claim 15
Dependent Claim
24 words
A DNA expression vector comprising a Saccharomyces cerevisiae pyruvate kinase promoter followed by a gene expressing a foreign protein under regulation of said protein.
Claim 16
Dependent Claim
24 words
A DNA expression vector comprising a Saccharomyces cerevisiae pyruvate kinase promoter followed by a gene expressing a foreign protein under regulation of said protein.
Claim 17
Independent Claim
43 words
A method for preparing any foreign protein which comprises: introducing into Saccharomyces cervisiae, a DNA expression vector according to claims 1, 2, 3, or 4; and growing said Saccharomyces cervisiae in an appropriate medium and isolating the protein expressed by said foreign DNA.
Claim 18
Dependent Claim
27 words
A Saccharomyces cerevisiae said promoter and said gene integrated into its genome from a DNA expression vector according to any of claims 1, 2, 3 or 4.
Claim 19
Dependent Claim
17 words
A Saccharomyces cervisiae cell according to claim 15, wherein said promoter is the triose phosphate isomerase promoter.
Claim 20
Dependent Claim
16 words
A Saccharomyces cerevisiae cell according to claim 15, wherein said promoter is the pyruvate kinase promoter.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-07-07 | 1990-01-06 | 1990-07-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-07-07 | 1994-01-06 | 1994-07-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-07-07 | 1998-01-06 | 1998-07-08 | $7700.00 | Window Closed / Lapsed |