US5366875A
Expired / Lapsed
US
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Methods for producing BMP-7 proteins
Assignee: Genetics Institute LLC
•
Filing: 1991-09-24
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Granted: 1994-11-22
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Expires: 2011-09-24
Abstract
Abstract Purified BMP-7 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.
Inventors
- 👤 John M. Wozney
- 👤 Elizabeth A. Wang
- 👤 Vicki A. Rosen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:17
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; A; A61; A61L; A61L24/00; A61L24/04; A61L24/043; A61L27/00; A61L27/14; A61L27/22; A61L27/227; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A61K; A61K38/00
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
52 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence which encodes the amino acid sequence of SEQ ID NO: 8, culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 2
Independent Claim
52 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence which encodes the amino acid sequence of SEQ ID NO: 8, culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 3
Independent Claim
61 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence comprising the DNA sequence of SEQ ID NO: 7 which encodes the amino acid sequence of SEQ ID NO: 8, culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 4
Independent Claim
61 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence comprising the DNA sequence of SEQ ID NO: 7 which encodes the amino acid sequence of SEQ ID NO: 8, culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 5
Independent Claim
91 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence comprising the DNA sequence of SEQ ID NO: 7 and sequences which a) hybridize to the sequence of SEQ ID NO: 7 under stringent hybridization conditions; and b) express a protein characterized by the ability to induce the formation of cartilage and/or bone formation in the Rosen-modified Sampath Reddi assay culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 6
Independent Claim
91 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence comprising the DNA sequence of SEQ ID NO: 7 and sequences which a) hybridize to the sequence of SEQ ID NO: 7 under stringent hybridization conditions; and b) express a protein characterized by the ability to induce the formation of cartilage and/or bone formation in the Rosen-modified Sampath Reddi assay culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 7
Independent Claim
15 words
The method of claims 1, 2, or 3 wherein said mammalian cells are CHO cells.
Claim 8
Independent Claim
52 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence which encodes the amino acid sequence of SEQ ID NO: 10, culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 9
Independent Claim
52 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence which encodes the amino acid sequence of SEQ ID NO: 10, culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 10
Independent Claim
61 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence comprising the DNA sequence of SEQ ID NO: 9 which encodes the amino acid sequence of SEQ ID NO: 10, culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 11
Independent Claim
61 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence comprising the DNA sequence of SEQ ID NO: 9 which encodes the amino acid sequence of SEQ ID NO: 10, culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 12
Independent Claim
90 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence comprising the DNA sequence of SEQ ID NO: 9 and sequences which a) hybridize to the sequence of SEQ ID NO: 9 under stringent hybridization conditions; and b) express a protein characterized by the ability to induce the formation of catilage and/or bone in the Rosen-modified Sampath Reddi assay culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 13
Independent Claim
90 words
A method for producing a BMP-7 protein which comprises transforming mammalian cells with an expression vector, said vector having inserted therein a DNA sequence comprising the DNA sequence of SEQ ID NO: 9 and sequences which a) hybridize to the sequence of SEQ ID NO: 9 under stringent hybridization conditions; and b) express a protein characterized by the ability to induce the formation of catilage and/or bone in the Rosen-modified Sampath Reddi assay culturing said cells in a suitable culture medium, and isolating said BMP-7 protein produced by said cells.
Claim 14
Independent Claim
15 words
The method of claims 5, 6, or 7 wherein said mammalian cells are CHO cells.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1997-11-21 | 1998-05-23 | 1998-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-11-21 | 2002-05-23 | 2002-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-11-21 | 2006-05-23 | 2006-11-22 | $7700.00 | Window Closed / Lapsed |