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US5336599A
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US
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Method of measuring analyte using dry analytical element
Assignee: Fujifilm Holdings Corp
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Filing: 1993-12-15
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Granted: 1994-08-09
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Expires: 2013-12-15
Abstract
Abstract A method for measuring an analyte using a dry analytical element composed of at least a hydrophilic polymer layer and a spreading layer laminated onto a water-impermeable support and to which a liquid sample of the analyte has been supplied, by supplying a measuring reagent solution to the analytical element. The measurement of the analytical element is carried out by optical means.
Inventors
- 👤 Masao Kitajima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 14:11
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
144 words
A method for quantitatively measuring an analyte using a dry analytical element which comprises: supplying a measuring reagent solution to an analytical element comprising at least a hydrophilic polymer layer and a spreading layer which functions to evenly spread components contained in an aqueous liquid sample to supply the components to the hydrophilic polymer layer at a constant rate per unit area, said measuring reagent reacting directly with analyte in the liquid sample to produce a chemical change which is optically detectable, the analytical element having been prepared by applying a liquid sample comprising blood to the element and then drying the element under a drying temperature of from 10° to 60° C. up to a moisture content of the hydrophilic polymer of less than 20% to fix the sample thereon prior to supplying the reagent solution thereto; and optically measuring the chemical change.
Claim 2
Independent Claim
144 words
A method for quantitatively measuring an analyte using a dry analytical element which comprises: supplying a measuring reagent solution to an analytical element comprising at least a hydrophilic polymer layer and a spreading layer which functions to evenly spread components contained in an aqueous liquid sample to supply the components to the hydrophilic polymer layer at a constant rate per unit area, said measuring reagent reacting directly with analyte in the liquid sample to produce a chemical change which is optically detectable, the analytical element having been prepared by applying a liquid sample comprising blood to the element and then drying the element under a drying temperature of from 10° to 60° C. up to a moisture content of the hydrophilic polymer of less than 20% to fix the sample thereon prior to supplying the reagent solution thereto; and optically measuring the chemical change.
Claim 3
Dependent Claim
28 words
The method of claim 4 wherein the analytical element comprises at least a water-impermeable gas-permeable layer which is provided between the hydrophilic polymer layer and the spreading layer.
Claim 4
Dependent Claim
26 words
The method of claim 1 wherein the analytical element further comprised a light-shielding layer which is provided between the hydrophilic polymer layer and the spreading layer.
Claim 5
Dependent Claim
24 words
The method of claim 1 wherein the dry analytical element comprises a hydrophilic polymer layer and a spreading layer laminated onto a water-impermeable support.
Claim 6
Dependent Claim
24 words
The method of claim 4 wherein the analytical element further comprises a water-impermeable, gas-impermeable layer between the hydrophilic polymer layer and the spreading layer.
Claim 7
Dependent Claim
23 words
The method of claim 5 wherein the analytical element further comprises a light shield layer between the hydrophilic layer and the spreading layer.
Claim 8
Dependent Claim
14 words
The method of claim 1 wherein the blood sample is plasma or blood serum.
Claim 9
Dependent Claim
11 words
The method of claim 1 wherein the analyte is an enzyme.
Claim 10
Dependent Claim
18 words
The method of claim 1 wherein the element is dried at a temperature from 30° to 40° C.
Claim 11
Dependent Claim
12 words
The method of claim 9 wherein the analyte of GOT or GGT.
Claim 12
Dependent Claim
66 words
The method of claim 1 wherein the drying is carried out in an incubator composed of an element-receiving portion, a heating means, a cover which seals the element-receiving portion at the upper part of the receiving portion, and wherein the volume of the space formed in the element-receiving portion may be closed by a cover so as to conform to the volume of the analytical element.
Claim 13
Dependent Claim
21 words
The method of claim 1 wherein the moisture content of the hydrophilic polymer is less than 10% after the drying step.
Claim 14
Dependent Claim
31 words
The method of claim 1 wherein the element is maintained in a non-folded state from the step of applying the liquid sample to the step of supplying the measuring reagent solution.
References Cited (Backward Citations)
US3992158A
Cited
US4066403A
Cited
US4258001A
Cited
US4292272A
Cited
US4559949A
Cited
US4870005A
Cited
US4548906A
Cited
US4540670A
Cited
US4786595A
Cited
US4902477A
Cited
US4521520A
Cited
US4578358A
Cited
US4578359A
Cited
US5019347A
Cited
US4806311A
Cited
US4719181A
Cited
US4975366A
Cited
US4889797A
Cited
US4871679A
Cited
US4939085A
Cited
US4919890A
Cited
US5008078A
Cited
US5118472A
Cited
US5013527A
Cited
US5122451A
Cited
US5023052A
Cited
US4990457A
Cited
US5219762A
Cited
US5059349A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-08-08 | 1998-02-07 | 1998-08-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-08-08 | 2002-02-07 | 2002-08-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-08-08 | 2006-02-07 | 2006-08-09 | $7700.00 | Window Closed / Lapsed |