US4870005A
Expired / Lapsed
US
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Multilayer analysis element
Assignee: Fujifilm Holdings Corp
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Filing: 1985-10-16
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Granted: 1989-09-26
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Expires: 2005-10-16
Abstract
Abstract A dry type multilayer analysis element comprises at least one porous medium layer comprising a membrane filter, to which an antigen (or antibody) is immobilized, and at least one reagent layer through which a substance(s) which did not participate in an antigen-antibody reaction can permeate. The multilayer analysis element is effective for assaying components present in body fluids, blood, urine, etc., in a simple manner.
Inventors
- 👤 Yutaka Akiyoshi
- 👤 Asaji Kondo
- 👤 Masao Kitajima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 11:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54391; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/97; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 45
Claim 1
Independent Claim
104 words
A multilayer analysis element comprising: (A) a spreading layer consisting essentially of a textile; (B) a porous medium layer having immobilized therein a substance which causes a competitive antigen-antibody reaction to form a bound antigen-antibody complex; and (C) a reagent layer which permeates an unbound free antigen or antibody, wherein the porous medium layer is selected from the group consisting of a membrane filter, woven or knitted fabrics, a non-woven fabric and a water-permeable paper and is interposed between said spreading layer and said reagent layer and the reagent layer comprises a porous material and, as a binder a hydrophilic high molecular weight substance
Claim 2
Dependent Claim
104 words
A multilayer analysis element comprising: (A) a spreading layer consisting essentially of a textile; (B) a porous medium layer having immobilized therein a substance which causes a competitive antigen-antibody reaction to form a bound antigen-antibody complex; and (C) a reagent layer which permeates an unbound free antigen or antibody, wherein the porous medium layer is selected from the group consisting of a membrane filter, woven or knitted fabrics, a non-woven fabric and a water-permeable paper and is interposed between said spreading layer and said reagent layer and the reagent layer comprises a porous material and, as a binder a hydrophilic high molecular weight substance
Claim 3
Dependent Claim
34 words
The multilayer analysis element as claimed in claim 1, wherein the substance which causes a competitive antigen-antibody reaction to form a bound antigen-antibody complex is immobilized in the interior of said porous medium layer.
Claim 4
Dependent Claim
30 words
The multilayer analysis element as claimed in claim 1 or 2 wherein said porous medium layer comprises a porous material having a void of from about 20 to about 90%.
Claim 5
Dependent Claim
25 words
The multilayer analysis element as claimed in claim 3 wherein said porous medium layer has a thickness of about 50 μm to about 1 mm.
Claim 6
Dependent Claim
24 words
The multilayer analysis element as claimed in claim 1 wherein said textile is a plain weave formed by weaving warp and weft yarns alternately.
Claim 7
Dependent Claim
25 words
The multilayer analysis element as claimed in claim 1 wherein said porous medium layer has a thickness of about 50 μm to about 1 mm.
Claim 8
Dependent Claim
22 words
The multilayer analysis element as claimed in claim 1 wherein said reagent layer has a thickness of 5 to about 200 μm.
Claim 9
Dependent Claim
25 words
The. multilayer analysis element as claimed in claim 1 wherein a radiation blocking layer is provided between said porous medium layer and said reagent layer.
Claim 10
Dependent Claim
32 words
The multilayer analysis element as claimed in claim 8 wherein said radiation blocking layer is a light reflection or absorption layer and has a thickness of about 1 to about 50 μm.
Claim 11
Dependent Claim
32 words
The multilayer analysis element as claimed in claim 8 or 9 wherein said radiation blocking layer comprises a hydrophilic high molecular weight substance containing 0.5 to 20 wt % of carbon black.
Claim 12
Dependent Claim
25 words
The multilayer analysis element as claimed in claim 1 wherein 0.1 to 10 wt % of carbon black is contained in said porous medium layer.
Claim 13
Dependent Claim
41 words
The multilayer analysis element as claimed in claim 1 wherein said substance causing a competitive antigen-antibody reaction is an antibody or antigen and said substance which did not undergo the antigen-antibody reaction is an unreacted labelled antigen or unreacted labelled antibody.
Claim 14
Dependent Claim
24 words
The multilayer analysis element as claimed in claim 1 wherein the hydrophilic high molecular weight substance is a natural hydrophilic high molecular weight substance.
Claim 15
Dependent Claim
33 words
The multilayer analysis element as claimed in claim 13, wherein the natural hydrophilic high molecular weight substance is selected from the group consisting of gelatin, agarose, sodium alginate, carboxymethyl, cellulose and methyl cellulose.
Claim 16
Dependent Claim
46 words
The multilayer analysis element as claimed in claim 1 wherein the hydrophilic high molecular weight substance is a synthetic hydrophilic high molecular weight substance selected from the group consisting of polyacryl mide, polyvinyl alcohol, polyvinyl pyrrolidone, sodium polyacrylate, poly hydroxyethyl methacrylate, and copolymers containing maleic acid.
Claim 17
Dependent Claim
32 words
The multilayer analysis element as claimed in claim 15 wherein the synthetic hydrophilic high molecular weight substance is selected from the group consisting of polyacrylamide, sodium polyacrylate and copolymers containing acrylic acid.
Claim 18
Dependent Claim
17 words
The multilayer analysis element as claimed in claim 1, wherein the porous medium is a membrane filter.
Claim 19
Dependent Claim
18 words
The multilayer analysis element as claimed in claim 1, wherein the textile of the spreading layer is hydrophilic.
Claim 20
Dependent Claim
17 words
The multilayer analysis element as claimed in claim 1, wherein the hydrophilic high molecular substance is polyacrylamide.
Claim 21
Dependent Claim
33 words
The multilayer analysis element as claimed in claim 1, further comprising a transparent support which is light-transmitting and which supports in sequence the reagent layer, the porous medium layer and the spreading layer.
Claim 22
Dependent Claim
18 words
The multilayer analysis element as claimed in claim 20, wherein the porous medium layer is a membrane filter.
Claim 23
Dependent Claim
18 words
The multilayer analysis element as claimed in claim 20, wherein the textile of the spreading layer is hydrophilic.
Claim 24
Dependent Claim
18 words
The multilayer analysis element as claimed in claim 20, wherein the hydrophilic high molecular weight substance is polyacrylamide.
Claim 25
Dependent Claim
34 words
The multilayer analysis element as claimed in claim 20, wherein the substance which causes a competitive antigen-antibody reaction to form a bound antigen-antibody complex is immobilized in the interior of the porous medium layer
Claim 26
Dependent Claim
25 words
The multilayer analysis element as claimed in claim 20, wherein a radiation blocking layer is provided between the porous medium layer and the reagent layer.
Claim 27
Dependent Claim
32 words
The multilayer analysis element as claimed in claim 20, wherein the hydrophilic high molecular weight substance is selected from the group consisting of gelatin, agarose, sodium alginate, carboxymethyl cellulose and methyl cellulose
Claim 28
Dependent Claim
45 words
The multilayer analysis element as claimed in claim 20, wherein the hydrophilic high molecular weight substance is a synthetic hydrophilic high molecular weight substance selected from the group consisting of polyacrylamide, polyvinyl alcohol, polyvinyl pyrrolidone, sodium polyacrylate, poly hydroxyethyl methacrylate, and copolymers containing maleic acid.
Claim 29
Dependent Claim
32 words
The multilayer analysis element as claimed in claim 20, wherein the synthetic hydrophilic high molecular weight substance is selected from the group consisting of polyacrylamide, sodium polyacrylate and polymers containing acrylic acid.
Claim 30
Dependent Claim
63 words
An integral multilayer analysis element comprising: (a) a porous medium layer having immobilized therein a substance which causes a competitive antigen-antibody reaction to form a bound antigen-antibody complex, and (b) a reagent layer which permeates an unbound free antigen or antibody, wherein said porous medium layer comprises a textile and said reagent layer comprises a hydrophilic high molecular weight substance as a binder.
Claim 31
Dependent Claim
63 words
An integral multilayer analysis element comprising: (a) a porous medium layer having immobilized therein a substance which causes a competitive antigen-antibody reaction to form a bound antigen-antibody complex, and (b) a reagent layer which permeates an unbound free antigen or antibody, wherein said porous medium layer comprises a textile and said reagent layer comprises a hydrophilic high molecular weight substance as a binder.
Claim 32
Dependent Claim
35 words
The multilayer analysis element as claimed in claim 29, wherein a transparent support having a light transmitting capability is provided on the back surface opposite the surface on which the porous medium layer is provided.
Claim 33
Dependent Claim
24 words
The multilayer analysis element as claimed in claim 29, wherein said textile is a plain weave formed by weaving warp and weft yarns alternately.
Claim 34
Dependent Claim
34 words
The multilayer analysis element as claimed in claim 29, wherein the substance which causes a competitive antigen-antibody reaction to form a bound antigen-antibody complex is immobilized in the interior of the porous medium layer.
Claim 35
Dependent Claim
25 words
The multilayer analysis element as claimed in claim 29, wherein a radiation blocking layer is provided between the porous medium layer and the reagent layer.
Claim 36
Dependent Claim
32 words
The multilayer analysis element as claimed in claim 29, wherein the hydrophilic high molecular weight substance is selected from the group consisting of gelatin, agarose, sodium alginate, carboxylmethyl cellulose and methyl cellulose.
Claim 37
Dependent Claim
56 words
The multilayer analysis element as claimed in claim 29, wherein the reagent layer is a hydrophilic high molecular weight substance and said hydrophilic high molecular weight substance is a synthetic hydrophilic high molecular weight substance selected from the group consisting of polyacrylamide, polyvinyl alcohol, polyvinyl pyrrolidone, sodium polyacrylate, poly hydroxyethyl methacrylate, and copolymers containing maleic acid.
Claim 38
Dependent Claim
42 words
The multilayer analysis element as claimed in claim 31 wherein the reagent layer is a hydrophilic high molecular weight substance and said hydrophilic high molecular weight substance is selected from the group consisting of polyacrylamide, sodium polyacrylate and polymers containing acrylic acid.
Claim 39
Dependent Claim
29 words
The multilayer analysis element as claimed in claim 29 wherein the reagent layer is a hydrophilic high molecular weight substance and said hydrophilic high molecular weight substance is polyacrylamide.
Claim 40
Dependent Claim
100 words
A multilayer analysis element comprising: (A) a spreading layer consisting essentially of a textile; (B) a porous medium layer having immobilized therein a substance which causes a competitive antigen-antibody reaction to form a bound antigen-antibody complex; and (C) a reagent layer which permeates an unbound free antigen or antibody, wherein the porous medium layer is selected from the group consisting of a membrane filter, woven or knitted fabrics, a non-woven fabric and a water-permeable paper and is interposed between said spreading layer and said reagent layer and the reagent layer comprises a hydrophilic high molecular weight substance as a binder.
Claim 41
Dependent Claim
100 words
A multilayer analysis element comprising: (A) a spreading layer consisting essentially of a textile; (B) a porous medium layer having immobilized therein a substance which causes a competitive antigen-antibody reaction to form a bound antigen-antibody complex; and (C) a reagent layer which permeates an unbound free antigen or antibody, wherein the porous medium layer is selected from the group consisting of a membrane filter, woven or knitted fabrics, a non-woven fabric and a water-permeable paper and is interposed between said spreading layer and said reagent layer and the reagent layer comprises a hydrophilic high molecular weight substance as a binder.
Claim 42
Dependent Claim
33 words
The multilayer analysis element as claimed in claim 38, further comprising a transparent support which is light-transmitting and which supports in sequence the reagent layer, the porous medium layer and the spreading layer.
Claim 43
Dependent Claim
25 words
The multilayer analysis element as claimed in claim 38, wherein a radiation blocking layer is provided between said porous medium layer and said reagent layer.
Claim 44
Dependent Claim
32 words
The multilayer analysis element as claimed in claim 38, wherein the hydrophilic high molecular weight substance is selected from the group consisting of gelatin, agarose, sodium alginate, carboxylmethyl cellulose and methyl cellulose.
Claim 45
Dependent Claim
45 words
The multilayer analysis element as claimed in claim 38, wherein the hydrophilic high molecular weight substance is a synthetic hydrophilic high molecular weight substance selected from the group consisting of polyacrylamide, polyvinyl alcohol, polyvinyl pyrrolidone, sodium polyacrylate, poly hydroxyethyl methacrylate, and copolymers containing maleic acid.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-09-25 | 1993-03-27 | 1993-09-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-09-25 | 1997-03-27 | 1997-09-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-25 | 2001-03-27 | 2001-09-26 | $7700.00 | Window Closed / Lapsed |