US4975366A
Expired / Lapsed
US
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Multi-layered element for quantititative analysis of immuno reactant
Assignee: Fujifilm Holdings Corp
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Filing: 1987-02-18
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Granted: 1990-12-04
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Expires: 2007-02-18
Abstract
Abstract A multi-layered element for the quantative analysis of an immuno-reactant having a water-impermeable and light-transmissible support layer; a coloring reagent layer carried by said support layer and containing a hydrophilic polymer as a binder; and a reaction layer covering said reagent layer and made of a porous matrix. When the analyte immunoreactant is an antigen, said reaction layer contains the specific antibody for the antigen, the antibody being immobilized by a first water-insoluble carrier. When the analyte immuno-reactant is an antibody, said reaction layer contains the specific antigen for the antibody. The reaction layer contains an enzyme substrate immobilized by a second water-insoluble carrier different from said first water-insoluble carrier. The coloring reagent layer contains a detection reagent composition for coupling with an enzymatic reaction product produced by the reaction beween the enzyme-labelled complex of the immuno-reactant and the enzyme substrate immobilized by the second water-insoluble carrier. Upon coupling of the detection reagent composition with the enzymatic reaction product, a color is developed, the optical density of which is measured to determine the quantity of the analyte immuno-reactant contained in a liquid sample.
Inventors
- 👤 Yukio Sudo
- 👤 Nobuhito Masuda
- 👤 Kenji Miura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 13:23
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54391; G01N33/52; G01N33/525; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
132 words
A multi-layered analytical element for the quantitative analysis of an analyte antigen, mixed with an enzyme-labelled antigen in a sample, comprising: a water-impermeable and light-transmissible support; a reagent layer carried by said support and containing a hydrophilic polymer aS a binder; and a reaction layer covering said reagent layer and made of a porous matrix; said reaction layer containing an antibody for the antigen and a substrate for the enzyme of the enzyme labelled antigen, said antibody being immobilized by a first water-insoluble carrier, said substrate being immobilized by a second water-insoluble carrier which is different from said first water-insoluble carrier; said reagent layer containing a detection reagent composition for coupling with an enzymatic reaction product produced from the reaction of the enzymatic label and substrate therefor to develop a measurable color.
Claim 2
Dependent Claim
132 words
A multi-layered analytical element for the quantitative analysis of an analyte antigen, mixed with an enzyme-labelled antigen in a sample, comprising: a water-impermeable and light-transmissible support; a reagent layer carried by said support and containing a hydrophilic polymer aS a binder; and a reaction layer covering said reagent layer and made of a porous matrix; said reaction layer containing an antibody for the antigen and a substrate for the enzyme of the enzyme labelled antigen, said antibody being immobilized by a first water-insoluble carrier, said substrate being immobilized by a second water-insoluble carrier which is different from said first water-insoluble carrier; said reagent layer containing a detection reagent composition for coupling with an enzymatic reaction product produced from the reaction of the enzymatic label and substrate therefor to develop a measurable color.
Claim 3
Dependent Claim
39 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 1, wherein said reaction layer further contains said enzyme-labelled antigen so that analyte antigen in the sample is mixed with said enzyme-labelled antigen therein.
Claim 4
Dependent Claim
39 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 1, wherein said reagent layer further contains said enzyme-labelled antigen so that analyte antigen in the sample is mixed with said enzyme-labelled antigen therein.
Claim 5
Dependent Claim
46 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 1, wherein said reaction layer is covered by a spreading layer containing said enzyme-labelled antigen so that analyte antigen in the sample is mixed with enzyme-labelled antigen in said spreading layer.
Claim 6
Dependent Claim
72 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 1, 3 or 4, wherein said reaction layer comprises a first reaction layer containing said antibody immobilized by said first water-insoluble carrier and a second reaction layer containing said enzyme substrate immobilized by said second water-insoluble carrier, and wherein said second reaction layer covers said reagent layer and said first reaction layer covers said second reaction layer.
Claim 7
Dependent Claim
70 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 2, wherein said reaction layer comprises: a first reaction layer containing said antibody immobilized by said first water-insoluble carrier, and said enzyme-labelled antigen; and a second reaction layer containing said enzyme substrate immobilized by said second water-insoluble carrier; said second reaction layer covering said reagent layer, and said first layer covering said second reaction layer.
Claim 8
Dependent Claim
31 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 1, 2, 3, or 4, wherein said reaction layer further contains micro particles for shielding colors.
Claim 9
Dependent Claim
28 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 5 wherein said second reaction layer further contains micro particles for shielding colors
Claim 10
Dependent Claim
28 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 1, 2, 3, or 4, wherein said porous matrix is a fibrous material.
Claim 11
Dependent Claim
28 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 1, 2, 3, or 4, wherein said porous matrix is a non-fibrous material.
Claim 12
Dependent Claim
30 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 1, 2, 3, or 4, wherein said porous matrix is composed of said water-soluble carrier.
Claim 13
Dependent Claim
28 words
The multi-layered element for the quantitative analysis of an analyte antigen as claimed in claim 6, wherein said second reaction layer further contains micro particles for shielding colors.
Claim 14
Dependent Claim
130 words
A multi-layered element for the quantitative analysis of an analyte antibody, mixed with an enzyme-labelled antibody in a sample, comprising: a water-impermeable and light-transmissible support; a reagent layer carried by said support and containing a hydrophilic polymer as a binder; and a reaction layer covering said reagent layer and made of a porous matrix; said reaction layer containing an antigen for the antibody and a substrate for the enzyme of the enzyme-labelled antibody, said antigen being immobilized by a first water-soluble carrier, said substrate being immobilized by a second water-insoluble carrier which is different from said first water-insoluble carrier; said reagent layer containing a detection reagent composition for coupling with an enzymatic reaction product produced from the reaction of the enzymatic label and substrate therefor to develop a measurable color.
Claim 15
Dependent Claim
130 words
A multi-layered element for the quantitative analysis of an analyte antibody, mixed with an enzyme-labelled antibody in a sample, comprising: a water-impermeable and light-transmissible support; a reagent layer carried by said support and containing a hydrophilic polymer as a binder; and a reaction layer covering said reagent layer and made of a porous matrix; said reaction layer containing an antigen for the antibody and a substrate for the enzyme of the enzyme-labelled antibody, said antigen being immobilized by a first water-soluble carrier, said substrate being immobilized by a second water-insoluble carrier which is different from said first water-insoluble carrier; said reagent layer containing a detection reagent composition for coupling with an enzymatic reaction product produced from the reaction of the enzymatic label and substrate therefor to develop a measurable color.
Claim 16
Dependent Claim
39 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 13, wherein said reaction layer further contains said enzyme-labelled antibody so that analyte antibody in the sample is mixed with said enzyme-labelled antibody therein.
Claim 17
Dependent Claim
39 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 13, wherein said reagent layer further contains said enzyme-labelled antibody so that analyte antibody in the sample is mixed with said enzyme-labelled antibody therein.
Claim 18
Dependent Claim
46 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 13, wherein said reaction layer is covered by a spreading layer containing said enzyme-labelled antibody so that analyte antibody in the sample is mixed with enzyme-labelled antibody in said spreading layer.
Claim 19
Dependent Claim
72 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 13, 14, or 16, wherein said reaction layer comprises a first reaction layer containing said antigen immobilized by said first water-insoluble carrier and a second reaction layer containing said enzyme substrate immobilized by said second water-insoluble carrier, and wherein said second reaction layer covers said reagent layer and said first reaction layer covers said second reaction layer.
Claim 20
Dependent Claim
70 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 14, wherein said reaction layer comprises: a first reaction layer containing said antigen immobilized by said first water-insoluble carrier, and said enzyme-labelled antibody; and a second reaction layer containing said enzyme substrate immobilized by said second water-insoluble carrier; said second reaction layer covering said reagent layer, and said first layer covering said second reaction layer.
Claim 21
Dependent Claim
31 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in any claim 14, 15, or 16, wherein said reaction layer further contains micro particles for shielding colors.
Claim 22
Dependent Claim
28 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 17 wherein said second reaction layer further contains micro particles for shielding colors.
Claim 23
Dependent Claim
27 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 14, 15, or 16, wherein said porous matrix is a fibrous material.
Claim 24
Dependent Claim
27 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 14, 15, or 16, wherein said porous matrix is a non-fibrous material.
Claim 25
Dependent Claim
29 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 14, 15, or 16, wherein said porous matrix is composed of said water-insoluble carrier.
Claim 26
Dependent Claim
28 words
The multi-layered element for the quantitative analysis of an analyte antibody as claimed in claim 18, wherein said second reaction layer further contains micro particles for shielding colors.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-12-03 | 1994-06-04 | 1994-12-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-12-03 | 1998-06-04 | 1998-12-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-12-03 | 2002-06-04 | 2002-12-04 | $7700.00 | Window Closed / Lapsed |