US5336599A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of measuring analyte using dry analytical element
Assignee: Fujifilm Holdings Corp
•
Filing: 1993-12-15
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Granted: 1994-08-09
•
Expires: 2013-12-15
Abstract
Abstract A method for measuring an analyte using a dry analytical element composed of at least a hydrophilic polymer layer and a spreading layer laminated onto a water-impermeable support and to which a liquid sample of the analyte has been supplied, by supplying a measuring reagent solution to the analytical element. The measurement of the analytical element is carried out by optical means.
Inventors
- 👤 Masao Kitajima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 02:02
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
144 words
A method for quantitatively measuring an analyte using a dry analytical element which comprises: supplying a measuring reagent solution to an analytical element comprising at least a hydrophilic polymer layer and a spreading layer which functions to evenly spread components contained in an aqueous liquid sample to supply the components to the hydrophilic polymer layer at a constant rate per unit area, said measuring reagent reacting directly with analyte in the liquid sample to produce a chemical change which is optically detectable, the analytical element having been prepared by applying a liquid sample comprising blood to the element and then drying the element under a drying temperature of from 10° to 60° C. up to a moisture content of the hydrophilic polymer of less than 20% to fix the sample thereon prior to supplying the reagent solution thereto; and optically measuring the chemical change.
Claim 2
Independent Claim
144 words
A method for quantitatively measuring an analyte using a dry analytical element which comprises: supplying a measuring reagent solution to an analytical element comprising at least a hydrophilic polymer layer and a spreading layer which functions to evenly spread components contained in an aqueous liquid sample to supply the components to the hydrophilic polymer layer at a constant rate per unit area, said measuring reagent reacting directly with analyte in the liquid sample to produce a chemical change which is optically detectable, the analytical element having been prepared by applying a liquid sample comprising blood to the element and then drying the element under a drying temperature of from 10° to 60° C. up to a moisture content of the hydrophilic polymer of less than 20% to fix the sample thereon prior to supplying the reagent solution thereto; and optically measuring the chemical change.
Claim 3
Dependent Claim
28 words
The method of claim 4 wherein the analytical element comprises at least a water-impermeable gas-permeable layer which is provided between the hydrophilic polymer layer and the spreading layer.
Claim 4
Dependent Claim
26 words
The method of claim 1 wherein the analytical element further comprised a light-shielding layer which is provided between the hydrophilic polymer layer and the spreading layer.
Claim 5
Dependent Claim
24 words
The method of claim 1 wherein the dry analytical element comprises a hydrophilic polymer layer and a spreading layer laminated onto a water-impermeable support.
Claim 6
Dependent Claim
24 words
The method of claim 4 wherein the analytical element further comprises a water-impermeable, gas-impermeable layer between the hydrophilic polymer layer and the spreading layer.
Claim 7
Dependent Claim
23 words
The method of claim 5 wherein the analytical element further comprises a light shield layer between the hydrophilic layer and the spreading layer.
Claim 8
Dependent Claim
14 words
The method of claim 1 wherein the blood sample is plasma or blood serum.
Claim 9
Dependent Claim
11 words
The method of claim 1 wherein the analyte is an enzyme.
Claim 10
Dependent Claim
18 words
The method of claim 1 wherein the element is dried at a temperature from 30° to 40° C.
Claim 11
Dependent Claim
12 words
The method of claim 9 wherein the analyte of GOT or GGT.
Claim 12
Dependent Claim
66 words
The method of claim 1 wherein the drying is carried out in an incubator composed of an element-receiving portion, a heating means, a cover which seals the element-receiving portion at the upper part of the receiving portion, and wherein the volume of the space formed in the element-receiving portion may be closed by a cover so as to conform to the volume of the analytical element.
Claim 13
Dependent Claim
21 words
The method of claim 1 wherein the moisture content of the hydrophilic polymer is less than 10% after the drying step.
Claim 14
Dependent Claim
31 words
The method of claim 1 wherein the element is maintained in a non-folded state from the step of applying the liquid sample to the step of supplying the measuring reagent solution.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-08-08 | 1998-02-07 | 1998-08-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-08-08 | 2002-02-07 | 2002-08-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-08-08 | 2006-02-07 | 2006-08-09 | $7700.00 | Window Closed / Lapsed |