Patent US1840942A successfully refreshed from Google Patents.
Patent US5089390A successfully refreshed from Google Patents.
US5089390A
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US
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2-methyl-4-hexene- and 2-methyl-4-heptene-1,2-diol derivatives
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1987-09-04
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Granted: 1992-02-18
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Expires: 2007-09-04
Abstract
Abstract 2-methyl-4-hexene- and 3-methyl-5-heptene-1,2-diol derivatives are disclosed together with methods for preparing such derivatives. Where the derivative is a 2-methyl-4-hexene- or 3-methyl-5-heptene-1,2-diol conjugated to a label, the conjugates are useful in immunoassays. Where the 2-methyl-4-hexene or 3-methyl-5-heptene-1,2-diol is conjugated to an immunogenic carrier, the conjugates may be employed as an immunogen for use in the preparation of antibodies. The label conjugate and the antibodies can be utilized in an immunoassay for the determination or detection of cyclosporin in a sample suspected of containing cyclosporin.
Inventors
- 👤 Dariush Davalian
- 👤 Cheng-I Lin
- 👤 Edwin F. Ullman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 07:45
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/535; G01N33/94; G01N33/9493; Y; Y10; Y10S; Y10S435/00; Y10S435/964; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/822
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
133 words
In an assay for the determination of cyclosporin in a sample suspected of containing cyclosporin wherein the assay comprises the steps of (1) contacting the sample with antibodies for cyclosporin and a conjugate of a label and a compound recognized by said antibodies and (2) detecting immune complexes of said label conjugate and said antibodies, the improvement which comprises employing as said label conjugate a compound of the formula: ##STR10## wherein: X is a linking group of 0-65 atoms other than hydrogen; Z is a luminescent label selected from the group consisting of fluorogenic substrates, fluorescers, chemiluminescers, and fluorescent particles, or is an enzyme; Y is oxygen or sulfur; and n is a number from 1 up to the molecular weight of Z divided by 1000; and including the optically active isomers thereof.
Claim 2
Dependent Claim
133 words
In an assay for the determination of cyclosporin in a sample suspected of containing cyclosporin wherein the assay comprises the steps of (1) contacting the sample with antibodies for cyclosporin and a conjugate of a label and a compound recognized by said antibodies and (2) detecting immune complexes of said label conjugate and said antibodies, the improvement which comprises employing as said label conjugate a compound of the formula: ##STR10## wherein: X is a linking group of 0-65 atoms other than hydrogen; Z is a luminescent label selected from the group consisting of fluorogenic substrates, fluorescers, chemiluminescers, and fluorescent particles, or is an enzyme; Y is oxygen or sulfur; and n is a number from 1 up to the molecular weight of Z divided by 1000; and including the optically active isomers thereof.
Claim 3
Dependent Claim
14 words
The assay of claim 1 wherein said label is an enzyme or a fluorescer.
Claim 4
Dependent Claim
90 words
A kit for conducting an assay for the determination of cyclosporin, said kit comprising in packaged combination antibodies for cyclosporin and a compound of the formula: ##STR11## wherein: X is a linking group of 0-65 atoms other than hydrogen; Z is a luminescent label selected from the group consisting of fluorogenic substrates, fluorescers, chemiluminescers, and fluorescent particles, or is an enzyme; Y is oxygen or sulfur; and n is a number from 1 up to the molecular weight of Z divided by 1000; and including the optically active isomers thereof.
Claim 5
Dependent Claim
90 words
A kit for conducting an assay for the determination of cyclosporin, said kit comprising in packaged combination antibodies for cyclosporin and a compound of the formula: ##STR11## wherein: X is a linking group of 0-65 atoms other than hydrogen; Z is a luminescent label selected from the group consisting of fluorogenic substrates, fluorescers, chemiluminescers, and fluorescent particles, or is an enzyme; Y is oxygen or sulfur; and n is a number from 1 up to the molecular weight of Z divided by 1000; and including the optically active isomers thereof.
Claim 6
Dependent Claim
14 words
The kit of claim 3 wherein the label is an enzyme or a fluorescer.
Claim 7
Dependent Claim
123 words
The kit of claim 3 wherein said compound has the formula: ##STR12## wherein: X 1 is a linking group of from 0 to 35 atoms other than hydrogen, with the proviso that when X 1 contains more than 0 atoms at least 1 atom of X 1 is a carbon atom and that the remaining atoms are each independently selected from the group consisting of carbon, oxygen, nitrogen, and sulfur, wherein X 1 is bonded to 0 through a carbon atom and is bonded to Z 1 ; Z 1 is an enzyme of molecular weight greater than 5000; n 1 is a number from 1 up to the molecular weight of Z 1 divided by 1000; including the optically active isomers thereof.
Claim 8
Dependent Claim
11 words
The kit of claim 5 wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 9
Dependent Claim
58 words
The kit of claim 3 wherein said compound has the formula: ##STR13## wherein: W 1 is O, S, or NH; m is 0 to 10; Z 2 is an enzyme of molecular weight greater than 5000; n 2 is a number from 1 up to the molecular weight Z 2 divided by 1000; including the optical isomers thereof.
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