US4096237A
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Immunoassay for β-endorphin
Assignee: University of California Berkeley
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Filing: 1977-03-14
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Granted: 1978-06-20
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Expires: 1997-03-14
Abstract
Abstract A sensitive radioimmunoassay for β-endorphin (β-EP) is disclosed. It appears that the antigenic determinant resides in residues 6-15 of this molecule.
Inventors
- 👤 Choh Hao Li
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 15:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9486; C; C07; C07K; C07K14/00; C07K14/435; C07K14/665; C07K14/675; C07K16/00; C07K16/18; C07K16/26; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/823
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
11 words
An antigen comprising β-endorphin covalently bound to an immunogenic carrier material.
Claim 2
Dependent Claim
11 words
An antigen comprising β-endorphin covalently bound to an immunogenic carrier material.
Claim 3
Dependent Claim
14 words
The antigen of claim 1 where said β-endorphin is human β-endorphin (β h -endorphin).
Claim 4
Dependent Claim
14 words
The antigen of claim 1 wherein said β-endorphin is canoel β-endorphin (β c -endorphin).
Claim 5
Dependent Claim
14 words
The antigen of claim 1 wherein said immogenic carrier material is human gamma globulin.
Claim 6
Dependent Claim
2 words
125 I-β-endorphin.
Claim 7
Dependent Claim
2 words
125 I-β-endorphin.
Claim 8
Dependent Claim
11 words
The compound of claim 5 which is 125 I-β h -endorphin.
Claim 9
Dependent Claim
17 words
An antibody having the property of specifically binding β-endorphin or fragments thereof containing the (6-15) residue sequence.
Claim 10
Dependent Claim
17 words
An antibody having the property of specifically binding β-endorphin or fragments thereof containing the (6-15) residue sequence.
Claim 11
Dependent Claim
98 words
A radioimmunoassay for β-endorphin or a fragment thereof containing the (6-15) residue sequence which assay comprises incubating a mixture comprising the test sample containing an unknown amount of said β-endorphin or a fragment thereof containing the (6-15) residue sequence, a radiolabelled β-endorphin tracer compound and an antibody having the property of specifically binding β-endorphin or fragments thereof containing the (6-15) residue sequence; separating the free and the bound β-endorphin; counting the radioactivity from either the free or bound β-endorphin and determining the concentration of β-endorphin or fragment thereof in the test sample by reference to a standard curve.
Claim 12
Dependent Claim
98 words
A radioimmunoassay for β-endorphin or a fragment thereof containing the (6-15) residue sequence which assay comprises incubating a mixture comprising the test sample containing an unknown amount of said β-endorphin or a fragment thereof containing the (6-15) residue sequence, a radiolabelled β-endorphin tracer compound and an antibody having the property of specifically binding β-endorphin or fragments thereof containing the (6-15) residue sequence; separating the free and the bound β-endorphin; counting the radioactivity from either the free or bound β-endorphin and determining the concentration of β-endorphin or fragment thereof in the test sample by reference to a standard curve.
Claim 13
Dependent Claim
16 words
The assay of claim 8 wherein said radiolabelled β-endorphin tracer compound is 125 I-β h -endorphin.
Claim 14
Dependent Claim
20 words
The assay of claim 8 wherein said incubation is carried out at 4° C. for about 16 to 20 hours.
Claim 15
Dependent Claim
13 words
The assay of claim 8 wherein said test sample comprises β h -endorphin.
Claim 16
Dependent Claim
133 words
An immunoassay for β-endorphin or fragments thereof containing the (6-15) residue sequence which assay comprises incubating a mixture comprising the test sample containing an unknown amount of said β-endorphin or fragments thereof containing the (6-15) residue sequence, labeled β-endorphin or fragments thereof containing the (6-15) residue sequence and an antibody having the property of specifically binding β-endorphin or fragments thereof containing the (6-15) residue sequence, separating the free and the bound β-endorphin or fragments thereof containing the (6-15) residue sequence; measuring the amount of labeled β-endorphin or fragments thereof containing the (6-15) reside sequence from either the free or bound β-endorphin or fragments thereof containing the (6-15) residue sequence and determining the concentration of β-endorphin of fragments thereof containing the (6-15) residue sequence in the test sample by reference to a standard curve.
Claim 17
Dependent Claim
133 words
An immunoassay for β-endorphin or fragments thereof containing the (6-15) residue sequence which assay comprises incubating a mixture comprising the test sample containing an unknown amount of said β-endorphin or fragments thereof containing the (6-15) residue sequence, labeled β-endorphin or fragments thereof containing the (6-15) residue sequence and an antibody having the property of specifically binding β-endorphin or fragments thereof containing the (6-15) residue sequence, separating the free and the bound β-endorphin or fragments thereof containing the (6-15) residue sequence; measuring the amount of labeled β-endorphin or fragments thereof containing the (6-15) reside sequence from either the free or bound β-endorphin or fragments thereof containing the (6-15) residue sequence and determining the concentration of β-endorphin of fragments thereof containing the (6-15) residue sequence in the test sample by reference to a standard curve.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-06-19 | 1981-12-19 | 1982-06-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-06-19 | 1985-12-19 | 1986-06-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-06-19 | 1989-12-19 | 1990-06-20 | $7700.00 | Window Closed / Lapsed |