US4104367A
Expired / Lapsed
US
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Radioimmunoassay for methadone
Assignee: Hoffmann La Roche Inc
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Filing: 1976-10-13
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Granted: 1978-08-01
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Expires: 1996-10-13
Abstract
Abstract An improved immunoassay for methadone is disclosed. The subject radioimmunoassay utilizes a novel antigen, antibody and novel labelled methadone derivatives.
Inventors
- 👤 Magdalena Usategui Gomez
- 👤 Harvey Gurien
- 👤 John Edward Heveran
- 👤 Manfred Weigele
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 05:56
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/44; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9486; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/816
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
42 words
An antigen consisting essentially of a methadone derivative radical derived from a compound of the formula ##STR4## wherein φ is phenyl and n is an integer selected from 2 or 3 covalently bonded through the carboxyl group to an immunogenic carrier material.
Claim 2
Dependent Claim
42 words
An antigen consisting essentially of a methadone derivative radical derived from a compound of the formula ##STR4## wherein φ is phenyl and n is an integer selected from 2 or 3 covalently bonded through the carboxyl group to an immunogenic carrier material.
Claim 3
Dependent Claim
14 words
The antigen of claim 1 wherein said immunogenic carrier material is bovine serum albumin.
Claim 4
Dependent Claim
9 words
The angtigen of claim 1 wherein n is 2.
Claim 5
Independent Claim
119 words
A method for the radioimmunoassay of methadone in a sample which method comprises mixing said sample with a known amount of I 125 radiolabelled methadone derivative selected from the group consisting of I 125 -N-2-(4-hydroxyphenyl)-ethylsuccinamic acid N-methyl-N- [1-methyl-3,3-diphenyl-4-oxohexyl] aminoethanol ester and I 125 -6-dimethylamino-4-phenyl-4-(4-hydroxyphenyl)-hepten-3-one and an antibody which will selectively complex with methadone and said radiolabeled methadone derivative, measuring the degree of binding of the said labeled methadone derivative and determining the amount of methadone present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of methadone with fixed amounts of said labeled methadone derivative and said antibody and determining the degree of binding for each known amount of methadone.
Claim 6
Independent Claim
119 words
A method for the radioimmunoassay of methadone in a sample which method comprises mixing said sample with a known amount of I 125 radiolabelled methadone derivative selected from the group consisting of I 125 -N-2-(4-hydroxyphenyl)-ethylsuccinamic acid N-methyl-N- [1-methyl-3,3-diphenyl-4-oxohexyl] aminoethanol ester and I 125 -6-dimethylamino-4-phenyl-4-(4-hydroxyphenyl)-hepten-3-one and an antibody which will selectively complex with methadone and said radiolabeled methadone derivative, measuring the degree of binding of the said labeled methadone derivative and determining the amount of methadone present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of methadone with fixed amounts of said labeled methadone derivative and said antibody and determining the degree of binding for each known amount of methadone.
Claim 7
Dependent Claim
17 words
The method of claim 4 wherein said radiolabeled derivative is I 125 -N-2-(4-hydroxyphenyl)-ethylsuccinamic acid N-methyl-N-[1-methyl-3,3-diphenyl-4-oxohexyl] aminoethanol ester.
Claim 8
Dependent Claim
14 words
The method of claim 4 where said radiolabeled derivative is I 125 - 6-dimethylamino-4-phenyl-4-(4-hydroxyphenyl)heptan-3-one.
Claim 9
Dependent Claim
54 words
An antibody specific to methadone elicited by innoculating a host animal with an antigen consisting essentially of a methadone derivative radical derived from a compound of the formula ##STR5## wherein φ is phenyl and n is an integer selected from 2 or 3 covalently bonded through the carboxyl group to an immunogenic carrier material.
Claim 10
Dependent Claim
54 words
An antibody specific to methadone elicited by innoculating a host animal with an antigen consisting essentially of a methadone derivative radical derived from a compound of the formula ##STR5## wherein φ is phenyl and n is an integer selected from 2 or 3 covalently bonded through the carboxyl group to an immunogenic carrier material.
Claim 11
Dependent Claim
13 words
The antibody of claim 7 wherein the eliciting antigen has n of 2.
Claim 12
Dependent Claim
18 words
The antibody of claim 7 wherein the eliciting antigen has bovine serum albumin as the immunogenic carrier material.
Claim 13
Dependent Claim
8 words
I 125 -N-2-(4-hydroxyphenyl) ethylsuccinamic acid, N-methyl-N-[1-methyl-3,3-diphenyl-4-oxohexyl] aminoethanol ester.
Claim 14
Dependent Claim
8 words
I 125 -N-2-(4-hydroxyphenyl) ethylsuccinamic acid, N-methyl-N-[1-methyl-3,3-diphenyl-4-oxohexyl] aminoethanol ester.
Claim 15
Dependent Claim
3 words
I 125 -6-dimethylamino-4-phenyl-4-(4-hydroxyphenyl)hepten-3-one.
Claim 16
Dependent Claim
3 words
I 125 -6-dimethylamino-4-phenyl-4-(4-hydroxyphenyl)hepten-3-one.
Claim 17
Dependent Claim
20 words
A compound of the formula ##STR6## wherein φ is phenyl and n is an integer selected from 2 or 3.
Claim 18
Dependent Claim
20 words
A compound of the formula ##STR6## wherein φ is phenyl and n is an integer selected from 2 or 3.
Claim 19
Dependent Claim
9 words
The compound of claim 12 wherein n is 2.
Claim 20
Dependent Claim
15 words
A compound of the formula ##STR7## wherein φ is phenyl and acid addition salts thereof.
Claim 21
Dependent Claim
15 words
A compound of the formula ##STR7## wherein φ is phenyl and acid addition salts thereof.
Claim 22
Dependent Claim
11 words
N-2-(4-hydroxyphenyl) ethylsuccinamic acid, N-methyl-N-[1-methyl-3,3-diphenyl-4-oxohexyl] aminoethanol ester and acid addition salts thereof.
Claim 23
Dependent Claim
11 words
N-2-(4-hydroxyphenyl) ethylsuccinamic acid, N-methyl-N-[1-methyl-3,3-diphenyl-4-oxohexyl] aminoethanol ester and acid addition salts thereof.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-07-31 | 1982-01-30 | 1982-08-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-07-31 | 1986-01-30 | 1986-08-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-07-31 | 1990-01-30 | 1990-08-01 | $7700.00 | Window Closed / Lapsed |