US5008202A
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Blood diluent for red blood cell analysis
Assignee: SEQUOIA-TURNER Corp A CORP OF CA
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Filing: 1988-11-29
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Granted: 1991-04-16
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Expires: 2008-11-29
Abstract
Abstract Method for diluting blood, blood diluent, and antimicrobial agents and reagents, especially suitable for use in enumeration and sizing of blood cells. The antimicrobial agent in the blood diluent is potent, but relatively non-toxic to humans, and inexpensive. Furthermore, it does not interfere with blood analysis performed on an automated, blood-analysis instrument.
Inventors
- 👤 Sherburne M. Edmondson
- 👤 Carlos E. Luna
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 15:18
CPC Classifications
A; A01; A01N; A01N37/00; A01N37/44; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/108331
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
82 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising an aqueous solution of: (a) sodium sulfate; (b) sodium chloride; (c) 1, 3 dimethylurea; (d) 1-hydroxypyridine-2-thione; (e) EDTA; (f) ADA Buffer; and, (g) sodium hydroxide to obtain pH 6.9, wherein said diluent is an electrolyte capable of conducting current, stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 2
Dependent Claim
82 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising an aqueous solution of: (a) sodium sulfate; (b) sodium chloride; (c) 1, 3 dimethylurea; (d) 1-hydroxypyridine-2-thione; (e) EDTA; (f) ADA Buffer; and, (g) sodium hydroxide to obtain pH 6.9, wherein said diluent is an electrolyte capable of conducting current, stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 3
Dependent Claim
116 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising: (a) between 8.0 and 12.0 grams sodium sulfate, (b) between 3.0 and 6.0 grams sodium chloride; (c) between 0.5 and 3.0 grams 1,3 dimethylurea; (d) between 0.05 and 2.0 grams 1-hydroxypyridine-2-thione; (e) between 0.01 and 1.0 grams EDTA; (f) between 0.5 and 4.0 grams ADA Buffer; (g) sodium hydroxide to obtain pH 6.9; and (h) sufficient water to make one liter of diluent, wherein said diluent is an electrolyte capable of conducting current, stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 4
Dependent Claim
116 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising: (a) between 8.0 and 12.0 grams sodium sulfate, (b) between 3.0 and 6.0 grams sodium chloride; (c) between 0.5 and 3.0 grams 1,3 dimethylurea; (d) between 0.05 and 2.0 grams 1-hydroxypyridine-2-thione; (e) between 0.01 and 1.0 grams EDTA; (f) between 0.5 and 4.0 grams ADA Buffer; (g) sodium hydroxide to obtain pH 6.9; and (h) sufficient water to make one liter of diluent, wherein said diluent is an electrolyte capable of conducting current, stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 5
Dependent Claim
96 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising: (a) 10.0 grams sodium sulfate; (b) 4.2 grams sodium chloride; (c) 1.0 grams 1,3 dimethylurea; (e) 0.3 grams EDTA; (f) 1.4 grams ADA Buffer; (g) 0.5 grams sodium hydroxide to obtain pH 6.9; and (h) sufficient water to make one liter of diluent, wherein said diluent is an electrolyte capable of conducting current stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 6
Dependent Claim
96 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising: (a) 10.0 grams sodium sulfate; (b) 4.2 grams sodium chloride; (c) 1.0 grams 1,3 dimethylurea; (e) 0.3 grams EDTA; (f) 1.4 grams ADA Buffer; (g) 0.5 grams sodium hydroxide to obtain pH 6.9; and (h) sufficient water to make one liter of diluent, wherein said diluent is an electrolyte capable of conducting current stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 7
Dependent Claim
84 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populated and sub-populations in a whole blood sample, said diluent comprising an aqueous solution of: (a) sodium sulfate; (b) sodium chloride; (c) 1,3 dimethylurea; (d) 1-hydroxypyridine-2-thione; (e) EDTA; (f) sodium fluoride; (g) ADA Buffer; and, (h) sodium hydroxide to obtain pH 6.9, wherein said diluent is ;an electrolyte capable of conducting current, stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 8
Dependent Claim
84 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populated and sub-populations in a whole blood sample, said diluent comprising an aqueous solution of: (a) sodium sulfate; (b) sodium chloride; (c) 1,3 dimethylurea; (d) 1-hydroxypyridine-2-thione; (e) EDTA; (f) sodium fluoride; (g) ADA Buffer; and, (h) sodium hydroxide to obtain pH 6.9, wherein said diluent is ;an electrolyte capable of conducting current, stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 9
Dependent Claim
125 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising: (a) between 8.0 and 12.0 grams sodium sulfate; (b) between 3.0 and 6.0 grams sodium chloride ; (c) between 0.5 and 3.0 grams, 1,3 dimethylurea; (d) between 0.5 and 2.0 grams 1-hydroxypyridine-2-thione; (e) between 0.01 and 1.0 grams EDTA; (f) between 0.01 and 0.5 grams sodium fluoride; (g) between 0.5 and 4.0 grams ADA Buffer; (h) sodium hydroxide to obtain pH 6.9; and (i) sufficient water to make one liter of diluent, wherein said diluent is an electrolyte capable of conducting current stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 10
Dependent Claim
125 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising: (a) between 8.0 and 12.0 grams sodium sulfate; (b) between 3.0 and 6.0 grams sodium chloride ; (c) between 0.5 and 3.0 grams, 1,3 dimethylurea; (d) between 0.5 and 2.0 grams 1-hydroxypyridine-2-thione; (e) between 0.01 and 1.0 grams EDTA; (f) between 0.01 and 0.5 grams sodium fluoride; (g) between 0.5 and 4.0 grams ADA Buffer; (h) sodium hydroxide to obtain pH 6.9; and (i) sufficient water to make one liter of diluent, wherein said diluent is an electrolyte capable of conducting current stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 11
Dependent Claim
105 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising: (a) 10.0 grams sodium sulfate; (b) 4.2 grams sodium chloride; (c) 1.0 grams 1,3 di-methylurea; (d) 0.1 grams 1-hydroxypyridine-2-thione; (e) 0.3 grams EDTA; (f) 0.3 grams sodium fluoride; (g) 1.4 grams ADA Buffer; (h) 0.5 grams sodium hydroxide to obtain pH 6.9; and (i) sufficient water to make one liter of diluent, wherein said diluent is an electrolyte capable of conducting current, stabilizing red blood cell volumes and having no adverse effect on white blood cells.
Claim 12
Dependent Claim
105 words
A blood diluent useful in analysis of red blood cell number, and the enumeration of white blood cell populations and sub-populations in a whole blood sample, said diluent comprising: (a) 10.0 grams sodium sulfate; (b) 4.2 grams sodium chloride; (c) 1.0 grams 1,3 di-methylurea; (d) 0.1 grams 1-hydroxypyridine-2-thione; (e) 0.3 grams EDTA; (f) 0.3 grams sodium fluoride; (g) 1.4 grams ADA Buffer; (h) 0.5 grams sodium hydroxide to obtain pH 6.9; and (i) sufficient water to make one liter of diluent, wherein said diluent is an electrolyte capable of conducting current, stabilizing red blood cell volumes and having no adverse effect on white blood cells.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-04-15 | 1994-10-15 | 1995-04-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-04-15 | 1998-10-15 | 1999-04-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-04-15 | 2002-10-15 | 2003-04-16 | $7700.00 | Window Closed / Lapsed |