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US4213876A Expired / Lapsed US ⏱️ 0.0 years remaining

Multi-purpose blood diluent for use in electronic blood analysis instrumentation

Assignee: Coulter Electronics Inc
•
Filing: 1978-08-22
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Granted: 1980-07-22
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Expires: 1998-08-22
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Abstract

Abstract A multi-purpose blood diluent especially suitable for use in electronic enumeration and sizing of blood cells, determination of hemoglobin concentration and their collective indices and platelet parameters in a single blood sample by means of suitable electronic instrumentation. The diluent is characterized as azide-free and capable of affording accurate, reproducible hematological test results from the said suitable electronic instrumentation. The diluent employs a chelating agent which functions as a bacteriostat and fungistat agent to prevent adverse growth of micro-organisms and a compatible stabilizing agent to maintain desired morphology of red blood cells and an inorganic metallic sulfate for suppressing turbidity caused by abnormal globulins and/or high leukocyte counts which are encountered in certain blood samples. Because of the capability for use of the diluent in platelet determinations by the instrumentation, the diluent further is free of detergent action which would cause bubbles capable of interfering with the enumeration of platelets by the instrumentation. A modified and adjusted embodiment of the diluent enables its use in veterinary laboratory testing applications.

Inventors

  • 👤 Harold R. Crews
  • 👤 Dave Chastain, Jr.
  • 👤 Stephen L. Ledis

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-24 13:46
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; G01N15/00; G01N15/10; G01N15/1031; G01N15/12; G01N33/72; G01N33/721; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/105831; Y10T436/108331

Claims Analysis

Total Claims: 9
Claim 1
Independent Claim 111 words
A multi-purpose blood diluent suitable for use in electronic enumeration and sizing of blood cells, determination of hemoblobin concentration and their collective indices and platelet parameters in a single blood sample by means of electronic instrumentation comprising: A. an osmotically balanced solution of sodium chloride, a monobasic phosphate salt, a dibasic phosphate salt and sodium sulfate, B. a bacteriostatic agent comprising a hydroxyquinoline, and C. a hydrochloride compound selected from the family of anesthetic compounds compatible with said baceteriostatic agent for maintaining desired red blood cell morphology during operation of said instrumentation ; and D. said diluent being an aqueous electrolytic solution maintained within a preselected range of pH and osmolality.
Claim 2
Dependent Claim 111 words
A multi-purpose blood diluent suitable for use in electronic enumeration and sizing of blood cells, determination of hemoblobin concentration and their collective indices and platelet parameters in a single blood sample by means of electronic instrumentation comprising: A. an osmotically balanced solution of sodium chloride, a monobasic phosphate salt, a dibasic phosphate salt and sodium sulfate, B. a bacteriostatic agent comprising a hydroxyquinoline, and C. a hydrochloride compound selected from the family of anesthetic compounds compatible with said baceteriostatic agent for maintaining desired red blood cell morphology during operation of said instrumentation ; and D. said diluent being an aqueous electrolytic solution maintained within a preselected range of pH and osmolality.
Claim 3
Dependent Claim 29 words
The blood diluent of claim 1 in which said bacteriostatic agent consists of a compound selected from the group of 8-hydroxyquinoline, 8-hydroxyquinoline citrate, 8-hydroxyquinaldine, 8-hydroxy-5-nitroquinoline and 8-hydroxyquinoline-5-sulfonic acid dihydrate.
Claim 4
Dependent Claim 27 words
The blood diluent of claim 1 in which said hydrochloride compound is selected from the family of anesthetics consisting of procaine hydrochloride, mepivacaine hydrochloride and lidocaine hydrochloride.
Claim 5
Dependent Claim 13 words
The blood diluent of claim 1 in which said bacteriostatic agent comprises 8-quinolinol.
Claim 6
Dependent Claim 16 words
The blood diluent of claims 1 or 4 in which said hydrochloride compound comprises procaine hydrochloride.
Claim 7
Dependent Claim 16 words
The blood diluent of claim 1 in which the pH range is between 7.2 and 7.6.
Claim 8
Dependent Claim 17 words
The blood diluent of claim 6 in which the osmolality range is between 316 and 330 milliosmoles.
Claim 9
Dependent Claim 14 words
The blood diluent of claim 1 in which phosphate salts are sodium phosphate salts.

References Cited (Backward Citations)

US3549994A Cited
US3519572A Cited
US3920400A Cited
US3920580A Cited
US3873467A Cited
US3977995A Cited
US3962125A Cited
US4102810A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1983-07-22 1984-01-21 1984-07-22 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1987-07-22 1988-01-21 1988-07-22 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1991-07-22 1992-01-21 1992-07-22 $7700.00 Window Closed / Lapsed

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