US3873467A
Expired / Lapsed
US
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Hematologic reference control
Assignee: United Medical Laboratories Inc
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Filing: 1974-02-01
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Granted: 1975-03-25
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Expires: 1994-02-01
Abstract
Abstract A hematologic reference control provided for laboratory use in the calibration and control of automated and manual hematologic analyses. The reference control comprises a suspension of washed, stabilized human red blood cells in a nonproteinaceous aqueous suspension fluid that replaces the plasma in human blood. Stability in the reference control is attained by conditioning the cells by the inclusion in the aqueous suspension fluid of materials tending to make the cells assume a spherical shape without substantial change in the mean cell volume of the cells as well as imparting to the cells a resistance to the normal tendency of degrading with time. The aqueous suspension fluid furthermore produces an environment for the cells inhibiting biological activity. In a preferred embodiment there is further included in the reference control a minor amount of fixed human red blood cells, processed to have a substantially increased mean cell volume. The fixed cells are resistant to a change in cell volume, and to dissolution under the action of lysing agents producing lysing of the stabilized cells. The fixed red blood cells in the reference control substitute for the white cell population in human blood.
Inventors
- 👤 Roger A Hunt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:22
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N2400/00; G01N2400/10; G01N2400/12; G01N2400/14; G01N2400/22; G01N2496/00; G01N2496/05; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/106664; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
56 words
A HEMATOLOGIC REFERENCE CONTROL COMPRISING A SUSPENSION OF STABILIZED HUMAN RED BLOOD CELLS IN A NONPROTEINACEOUS AQUEOUS SUSPENSION FLUID, SAID BLOOD CELLS RETAINING ABILITY TO RESPOND TO A LYSING AGENT BY DISSOLUTION, AND BEING CONDITIONED IN SAID SUSPENSION BY THE INCLUSION, IN SAID SUSPENSION FLUID OF A DISULFONIC ACID SALT OF NAPHTHOL, AND AN ALKALINE TARTRATE.
Claim 2
Dependent Claim
56 words
A HEMATOLOGIC REFERENCE CONTROL COMPRISING A SUSPENSION OF STABILIZED HUMAN RED BLOOD CELLS IN A NONPROTEINACEOUS AQUEOUS SUSPENSION FLUID, SAID BLOOD CELLS RETAINING ABILITY TO RESPOND TO A LYSING AGENT BY DISSOLUTION, AND BEING CONDITIONED IN SAID SUSPENSION BY THE INCLUSION, IN SAID SUSPENSION FLUID OF A DISULFONIC ACID SALT OF NAPHTHOL, AND AN ALKALINE TARTRATE.
Claim 3
Dependent Claim
43 words
The reference control of claim 1, which further includes Dextran having a molecular weight within a range of 10,000 to 50, 000 dissolved in said suspension fluid, and imparting to the suspension fluid a specific gravity within the range of 1.01 to 1.03.
Claim 4
Dependent Claim
83 words
The reference control of claim 1, which further includes a minor amount of fixed human red blood cells, swollen to significantly greater mean cell volume than the mean cell volume of the stabilized cells and functioning as a substitute for the white cell population in human blood, the fixed cells having been fixed through an aldehyde treatment so as to be resistant to a change in mean cell volume, and resistant to dissolution by a lysing agent producing dissolution of the stabilized cells.
Claim 5
Dependent Claim
119 words
The reference control of claim 1, which further includes Dextran having a molecular weight within a range of 10,000 to 50, 000, which is dissolved in the suspension liquid and imparts to the suspension fluid a specific gravity within the range of 1.01 to 1.03, and a minor amount of fixed human red blood cells swollen to significantly greater mean cell volume than the mean cell volume of the stabilized red blood cells and functioning as a substitute for the white cell population in human blood, said fixed cells being fixed through an aldehyde treatment to be resistant to a change in mean cell volume and resistant to dissolution by a lysing agent producing dissolution of the stabilized cells.
Claim 6
Dependent Claim
117 words
In a hematologic reference control including a first group of human red blood cells suspended in an aqueous suspension fluid, a second group of human red blood cells suspended in said suspension fluid functioning to substitute for the white cell population of human blood, said second group of red blood cells consisting of cells in a swollen state whereby the cells of the second group have a significantly greater mean cell volume than the mean cell volume of the cells in the first group, the cells of the second group having been fixed in their swollen state whereby said cells are more resistant to change in cell volume and to dissolution than the remainder of the cells.
Claim 7
Dependent Claim
117 words
In a hematologic reference control including a first group of human red blood cells suspended in an aqueous suspension fluid, a second group of human red blood cells suspended in said suspension fluid functioning to substitute for the white cell population of human blood, said second group of red blood cells consisting of cells in a swollen state whereby the cells of the second group have a significantly greater mean cell volume than the mean cell volume of the cells in the first group, the cells of the second group having been fixed in their swollen state whereby said cells are more resistant to change in cell volume and to dissolution than the remainder of the cells.
Claim 8
Dependent Claim
25 words
The reference control of claim 5, wherein fixing of the cells of said second group is by submersing the cells in an aldehyde tanning solution.
Claim 9
Dependent Claim
53 words
A swelling fluid for the swelling of human red blood cells to increase their mean cell volume and to promote aggregation of the cells in their swollen state comprising an aqueous solution of sodium bicarbonate, iodoacetamide, an alkali tartrate, and Dextran having a molecular weight within a range of about 150,000 to 350,000.
Claim 10
Dependent Claim
53 words
A swelling fluid for the swelling of human red blood cells to increase their mean cell volume and to promote aggregation of the cells in their swollen state comprising an aqueous solution of sodium bicarbonate, iodoacetamide, an alkali tartrate, and Dextran having a molecular weight within a range of about 150,000 to 350,000.
Claim 11
Dependent Claim
32 words
A fixing fluid, for fixing swollen human red blood cells in their swollen state and imparting resistance to dissolution by lysing agents, comprising an aqueous solution of an alkali tartrate and glutaraldehyde.
Claim 12
Dependent Claim
32 words
A fixing fluid, for fixing swollen human red blood cells in their swollen state and imparting resistance to dissolution by lysing agents, comprising an aqueous solution of an alkali tartrate and glutaraldehyde.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-24 | 1978-09-23 | 1979-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-24 | 1982-09-23 | 1983-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-24 | 1986-09-23 | 1987-03-25 | $7700.00 | Window Closed / Lapsed |