US4390632A
Expired / Lapsed
US
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Stabilization process for biological cells and stabilized compositions thereof
Assignee: Coulter Electronics Inc
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Filing: 1980-07-14
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Granted: 1983-06-28
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Expires: 2000-07-14
Abstract
Abstract Biological cell compositions are stabilized to preserve aldehyde functionality by formation of acetal, particularly cyclic acetal, primarily in order to prevent cell-to-cell crosslinking. The stabilized biological cells are particularly useful as human blood particle analogs in reference reagents employed in electronic hematology testing instrumentation. A process for preparing the acetal-stabilized biological cells employs reaction of polyaldehyde-treated biological cells with alcohol, preferably including 1,2 dialcohol, under acidic conditions; thereafter, the resulting product solution is adjusted to basic condition, stabilizing the acetal formation on the biological cells.
Inventors
- 👤 II James H. Carter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:01
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/102499; Y10T436/107497
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
41 words
A stabilized biological cell composition treated to comprise at least one acetal radical selected from the group consisting of ##STR4## wherein R 1 , R 2 , R 3 and R 4 are groups having 1 to about 6 carbon atoms.
Claim 2
Dependent Claim
41 words
A stabilized biological cell composition treated to comprise at least one acetal radical selected from the group consisting of ##STR4## wherein R 1 , R 2 , R 3 and R 4 are groups having 1 to about 6 carbon atoms.
Claim 3
Dependent Claim
16 words
The composition as claimed in claim 1 wherein said acetal radical has said cyclic structure ##STR5##
Claim 4
Dependent Claim
28 words
The composition as claimed in claim 2 wherein said cyclic structure includes ##STR6## whereby R 3 is CH 2 and R 4 is CH 2 CH 2 .
Claim 5
Dependent Claim
26 words
The composition as claimed in claim 2 wherein said cyclic structure includes ##STR7## whereby R 3 is CH 2 and R 4 is CH 2 .
Claim 6
Dependent Claim
18 words
The composition as claimed in claim 1, 2, 3, or 4 wherein said biological cell includes animal erythrocyte.
Claim 7
Dependent Claim
23 words
The composition as claimed in claim 5 wherein said animal erythrocyte includes at least one member selected from human erythrocytes and goat erythrocytes.
Claim 8
Dependent Claim
15 words
The composition as claimed in claim 6 further comprising, suspension diluent containing said stabilized cells.
Claim 9
Dependent Claim
58 words
A process for stabilizing the fixation of biological cells treated with a polyaldehyde, in order to prevent cell-to-cell cross-linking of said cells, comprising: (a) reacting said aldehyde-treated biological cells with a least one alcohol in a solution maintained in acidic condition to form acetal, and thereafter, (b) adjusting the resulting product solution from step (a) to basic condition.
Claim 10
Dependent Claim
58 words
A process for stabilizing the fixation of biological cells treated with a polyaldehyde, in order to prevent cell-to-cell cross-linking of said cells, comprising: (a) reacting said aldehyde-treated biological cells with a least one alcohol in a solution maintained in acidic condition to form acetal, and thereafter, (b) adjusting the resulting product solution from step (a) to basic condition.
Claim 11
Dependent Claim
28 words
The process as claimed in claim 8 wherein said acidic reaction solution in step (a) is maintained at a pH in the range of approximately 2.3 to 2.9.
Claim 12
Dependent Claim
26 words
The process as claimed in claim 8 wherein said acidic reaction solution in step (a) is maintained by addition of a solution of glycine and HCl.
Claim 13
Dependent Claim
15 words
The process as claimed in claim 8 wherein said alcohol includes at least one 1,2-diol.
Claim 14
Dependent Claim
13 words
The process as claimed in claim 11 wherein said 1,2-diol includes ethylene glycol.
Claim 15
Dependent Claim
38 words
The process as claimed in claim 8 wherein said basic condition is effected in step (b) by addition of phosphate buffer solution in an amount sufficient to achieve a pH in the range of approximately 7.2 to 7.6.
Claim 16
Dependent Claim
18 words
The process as claimed in claim 8, 9, 10, 11, 12, or 13 wherein said polyaldehyde includes glutaraldehyde.
Claim 17
Dependent Claim
14 words
The process as claimed in claim 8 wherein said biological cells include animal erythrocytes.
Claim 18
Dependent Claim
32 words
The process as claimed in claim 15, wherein said animal erythrocytes include goat erythrocytes for use as human platelet analog in reference solutions suitable for use in electronic hematology laboratory testing instrumentation.
Claim 19
Dependent Claim
35 words
The process as claimed in claim 15, wherein said animal erythrocytes include human erythrocytes for use as a human white blood cell analog in reference solutions suitable for use in electronic hematology labratory testing instrumentation.
Claim 20
Dependent Claim
14 words
A stabilized biological cell composition prepared by the process as claimed in claim 8.
Claim 21
Dependent Claim
15 words
A suspension of stabilized biological cells prepared by the process as claimed in claim 8.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-27 | 1986-12-27 | 1987-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-27 | 1990-12-27 | 1991-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-27 | 1994-12-27 | 1995-06-28 | $7700.00 | Window Closed / Lapsed |