US4637986A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part WBC count
Assignee: Ortho Clinical Diagnostics Inc
•
Filing: 1983-08-22
•
Granted: 1987-01-20
•
Expires: 2003-08-22
Abstract
Abstract Lysing reagents for use in flow cytometry instruments. Lysing reagents suitable for lysing red blood cells are provided thereby permitting 3-part differential counting of white blood cells. The reagents comprise a leukoprotective agent for preserving the lymphocyte cellular integrity during analysis, and buffers adjusted to provide the correct pH environment for optimal lysis and activity. Preferred embodiments will additionally incorporate non-ionic preservatives in very low concentrations.
Inventors
- 👤 Michael C. Brown
- 👤 Stefan J. Kirchanski
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 23:23
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; G01N15/00; G01N15/10; G01N15/14; G01N15/1404; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
80 words
A lysing reagent for use in a flow cytometry instrument comprising: a combination of an organic, nitrogen containing buffer selected from the group consisting of TRIS and 2-amino-2-methyl-1,3-propanediol, and an ethane sulfonic acid buffer selected from the group consisting of HEPES and TAPS; and a leucoprotective agent selected from the group consisting of methyoxyethanol at a concentration of 100-700 mM, ethoxyethanol or methoxyethoxyethanol at a concentration of 50-500 mM, butoxyethanol or ethoxyethoxyethanol or butoxyethoxyethanol at a concentration of 10-300 mM.
Claim 2
Dependent Claim
80 words
A lysing reagent for use in a flow cytometry instrument comprising: a combination of an organic, nitrogen containing buffer selected from the group consisting of TRIS and 2-amino-2-methyl-1,3-propanediol, and an ethane sulfonic acid buffer selected from the group consisting of HEPES and TAPS; and a leucoprotective agent selected from the group consisting of methyoxyethanol at a concentration of 100-700 mM, ethoxyethanol or methoxyethoxyethanol at a concentration of 50-500 mM, butoxyethanol or ethoxyethoxyethanol or butoxyethoxyethanol at a concentration of 10-300 mM.
Claim 3
Dependent Claim
13 words
The reagent as provided in claim 1 wherein the leukoprotective agent is butoxyethanol.
Claim 4
Dependent Claim
26 words
The reagent as provided in claim 1 wherein the leukoprotective agent selected from Group B is present at approximately the optimal concentration listed in Table 1.
Claim 5
Dependent Claim
34 words
A lysing reagent comprising butoxyethanol present in the range of about 60 mM, TRIS buffer present in the range of about 5 mM and TAPS buffer present in the range of about 2.5 mM.
Claim 6
Dependent Claim
34 words
A lysing reagent comprising butoxyethanol present in the range of about 60 mM, TRIS buffer present in the range of about 5 mM and TAPS buffer present in the range of about 2.5 mM.
References Cited (Backward Citations)
US3956477A
Cited
US3874852A
Cited
US3874852B1
Cited
US4011045A
Cited
US4040785A
Cited
US4185964A
Cited
US4198206A
Cited
US4099917A
Cited
US4199471A
Cited
US4286963A
Cited
US4244837A
Cited
US4465774A
Cited
US4493821A
Cited
US4517301A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-01-19 | 1990-07-21 | 1991-01-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-01-19 | 1994-07-21 | 1995-01-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-01-19 | 1998-07-21 | 1999-01-20 | $7700.00 | Window Closed / Lapsed |