US4465774A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Standard or control material for glysocylated or total hemoglobin determination
Assignee: Covidien AG
•
Filing: 1981-07-16
•
Granted: 1984-08-14
•
Expires: 2001-07-16
Abstract
Abstract A control material for use in the determination of glycosylated and total hemoglobin, and a method of preparing the same, are disclosed. The control is prepared by the steps of hemolyzing red blood cells, preferably in the presence of a detergent, followed by removal of stroma and lipids from the hemoglobin with an organic solvent such as toluene in the presence of a polyhydroxy compound such as sucrose. A hemoglobin-rich fraction is separated from the resulting mixture and dialyzed against a solution of a polyhydroxy compound. The dialysate may be lyophilized to provide a stable, dry product suitable for reconstitution.
Inventors
- 👤 John J. Huang
- 👤 Jung-Shou Chen
- 👤 Louis M. Mezei
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 07:30
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/723; G01N33/96; G01N2333/00; G01N2333/795; G01N2333/805; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/104165; Y10T436/105831; Y10T436/106664; Y10T436/107497
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
84 words
A method of preparing a hemoglobin control material, said method comprising the steps of: (a) hemolyzing red blood cells to form a reaction mixture; (b) removing lipids and stroma from said reaction mixture by mixing said reaction mixture with an organic solvent immiscible with said reaction mixture and a first polyhydroxy compound to form a hemoglobin-rich first fraction, and a second fraction; (c) separating said first and second fractions; and, (d) removing residual organic solvent from said first fraction to obtain a hemoglobin-rich solution.
Claim 2
Independent Claim
84 words
A method of preparing a hemoglobin control material, said method comprising the steps of: (a) hemolyzing red blood cells to form a reaction mixture; (b) removing lipids and stroma from said reaction mixture by mixing said reaction mixture with an organic solvent immiscible with said reaction mixture and a first polyhydroxy compound to form a hemoglobin-rich first fraction, and a second fraction; (c) separating said first and second fractions; and, (d) removing residual organic solvent from said first fraction to obtain a hemoglobin-rich solution.
Claim 3
Dependent Claim
27 words
The method of claim 1 wherein said first polyhydroxy compound is selected from the class consisting of sucrose, sorbitol, glycerol, alkylene glycols, mono-saccharides, poly-saccharides and polyhydroxy carbohydrates.
Claim 4
Dependent Claim
12 words
The method of claim 2 wherein said first polyhydroxy compound is sucrose.
Claim 5
Dependent Claim
11 words
The method of claim 1 wherein said organic solvent is toluene.
Claim 6
Independent Claim
98 words
A method of preparing a hemoglobin control material, said method comprising the steps of: (a) hemolyzing red blood cells to form a reaction mixture; (b) removing lipids and stroma from said reaction mixture by mixing said reaction mixture with an organic solvent immiscible with said reaction mixture and a first polyhydroxy compound to form a hemoglobin-rich first fraction, and a second fraction; (c) separating said first and second fractions; and, (d) removing residual organic solvent from said first fraction by dialysis of said first fraction against a solution of a second polyhydroxy compound to obtain a hemoglobin-rich solution.
Claim 7
Independent Claim
98 words
A method of preparing a hemoglobin control material, said method comprising the steps of: (a) hemolyzing red blood cells to form a reaction mixture; (b) removing lipids and stroma from said reaction mixture by mixing said reaction mixture with an organic solvent immiscible with said reaction mixture and a first polyhydroxy compound to form a hemoglobin-rich first fraction, and a second fraction; (c) separating said first and second fractions; and, (d) removing residual organic solvent from said first fraction by dialysis of said first fraction against a solution of a second polyhydroxy compound to obtain a hemoglobin-rich solution.
Claim 8
Dependent Claim
15 words
The method of claim 5 wherein said second polyhydroxy compound comprises said first polyhydroxy compound.
Claim 9
Dependent Claim
41 words
The method of claim 5 wherein said second polyhydroxy compound differs from said first polyhydroxy compound, and said second polyhydroxy compound is substituted for said first polyhydroxy compound and said residual organic solvent in said first fraction in said dialysis step.
Claim 10
Independent Claim
102 words
A method of preparing a hemoglobin control material, said method comprising the consecutive steps of: (a) hemolyzing red blood cells by mixing said red blood cells with a detergent to form a reaction mixture; (b) mixing said reaction mixture with toluene and sucrose to form a hemoglobin-rich first fraction and a second fraction immiscible with said first fraction; (c) separating said first and second fractions; (d) dialyzing said first fraction against an aqueous sucrose solution to obtain a hemoglobin-rich dialysate comprising hemoglobin, water and sucrose; and, (e) lyophilizing said dialysate to provide a dry hemoglobin control material which is suitable for reconstitution.
Claim 11
Independent Claim
102 words
A method of preparing a hemoglobin control material, said method comprising the consecutive steps of: (a) hemolyzing red blood cells by mixing said red blood cells with a detergent to form a reaction mixture; (b) mixing said reaction mixture with toluene and sucrose to form a hemoglobin-rich first fraction and a second fraction immiscible with said first fraction; (c) separating said first and second fractions; (d) dialyzing said first fraction against an aqueous sucrose solution to obtain a hemoglobin-rich dialysate comprising hemoglobin, water and sucrose; and, (e) lyophilizing said dialysate to provide a dry hemoglobin control material which is suitable for reconstitution.
Claim 12
Dependent Claim
17 words
The method of claim 8 wherein said hemolysis ste is carried out in the presence of sucrose.
Claim 13
Dependent Claim
42 words
The method of claim 8 including the additional step of adjusting the optical density of said dialysate prior to lyophilization by addition of an aqueous sucrose solution thereto such that the optical density of said diluted dialysate approximates that of whole blood.
Claim 14
Independent Claim
95 words
A method of preparing a hemoglobin control material, said method comprising the steps of: (a) hemolyzing red blood cells in the presence of a first polyhydroxy compound to form a reaction mixture; (b) removing lipids and stroma from said reaction mixture by mixing said reaction mixture with an organic solvent immiscible with said reaction mixture in the presence of said first polyhydroxy compound to form a hemoglobin-rich first fraction, and a second fraction; (c) separating said first and second fractions; and, (d) removing residual organic solvent from said first fraction to obtain a hemoglobin-rich solution.
Claim 15
Independent Claim
95 words
A method of preparing a hemoglobin control material, said method comprising the steps of: (a) hemolyzing red blood cells in the presence of a first polyhydroxy compound to form a reaction mixture; (b) removing lipids and stroma from said reaction mixture by mixing said reaction mixture with an organic solvent immiscible with said reaction mixture in the presence of said first polyhydroxy compound to form a hemoglobin-rich first fraction, and a second fraction; (c) separating said first and second fractions; and, (d) removing residual organic solvent from said first fraction to obtain a hemoglobin-rich solution.
Claim 16
Dependent Claim
27 words
The method of claim 11 wherein said first polyhydroxy compound is selected from the class consisting of sucrose, sorbitol, glycerol, alkylene glycols, mono-saccharides, poly-saccharides and polyhydroxy carbohydrates.
Claim 17
Dependent Claim
12 words
The method of claim 12 wherein said first polyhydroxy compound is sucrose.
Claim 18
Independent Claim
126 words
A method of preparing a hemoglobin control material, said method comprising the consecutive steps of: (a) hemolyzing red blood cells by mixing said red blood cells with a detergent and a first polyhydroxy compound to form a reaction mixture; (b) mixing said reaction mixture with an organic solvent immiscible with said reaction mixture, and said first polyhydroxy compound to form a hemoglobin-rich first fraction and a second fraction immiscible with said first fraction; (c) separating said first and second fractions; (d) dialyzing said first fraction against an aqueous solution of a second polyhydroxy compound to obtain a hemoglobin-rich dialysate consisting essentially of hemoglobin, water and said second polyhydroxy compound; and, (e) lyophilizing said dialysate to provide a dry hemoglobin control material which is suitable for reconstitution.
Claim 19
Independent Claim
126 words
A method of preparing a hemoglobin control material, said method comprising the consecutive steps of: (a) hemolyzing red blood cells by mixing said red blood cells with a detergent and a first polyhydroxy compound to form a reaction mixture; (b) mixing said reaction mixture with an organic solvent immiscible with said reaction mixture, and said first polyhydroxy compound to form a hemoglobin-rich first fraction and a second fraction immiscible with said first fraction; (c) separating said first and second fractions; (d) dialyzing said first fraction against an aqueous solution of a second polyhydroxy compound to obtain a hemoglobin-rich dialysate consisting essentially of hemoglobin, water and said second polyhydroxy compound; and, (e) lyophilizing said dialysate to provide a dry hemoglobin control material which is suitable for reconstitution.
Claim 20
Dependent Claim
27 words
The method of claim 14 wherein said first polyhydroxy compound is selected from the class consisting of sucrose, sorbitol, glycerol, alkylene glycols, mono-saccharides, poly-saccharides and polyhydroxy carbohydrates.
Claim 21
Dependent Claim
12 words
The method of claim 15 wherein said first polyhydroxy compound is sucrose.
Claim 22
Dependent Claim
15 words
The method of claim 15 wherein said second polyhydroxy compound comprises said first polyhydroxy compound.
Claim 23
Dependent Claim
41 words
The method of claim 50 wherein said second polyhydroxy compound differs from said first polyhydroxy compound, and said second polyhydroxy compound is substituted for said first polyhydroxy compound and said residual organic solvent in said first fraction in said dialysis step.
References Cited (Backward Citations)
US3876375B1
Cited
US3876375A
Cited
US4056468A
Cited
US4215994A
Cited
US4102810A
Cited
US4001401A
Cited
US4011045A
Cited
BR7603767A
Cited
US4045176A
Cited
US3991181A
Cited
FR2345157A1
Cited
US4040785A
Cited
US4099917A
Cited
US4216117A
Cited
US4189401A
Cited
US4200435A
Cited
US4260516A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-14 | 1988-02-13 | 1988-08-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-14 | 1992-02-13 | 1992-08-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-14 | 1996-02-13 | 1996-08-14 | $7700.00 | Window Closed / Lapsed |