US4045176A
Expired / Lapsed
US
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Preparation of optically clear serum
Assignee: Individual
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Filing: 1976-03-29
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Granted: 1977-08-30
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Expires: 1996-03-29
Abstract
Abstract A stable optically clear serum is prepared for use in the preparation of standards and quality control reference material for the assay of human serum components. In one aspect of the present invention, high density lipoproteins isolated from human blood serum are added to substantially human serum to produce a standard or reference material. A second aspect of the present invention involves the addition of isolated, non-primate lipoproteins to substantially human serum. The serum prepared as disclosed has good optical clarity upon lyophilization and reconstitution with aqueous media. Alternatively, the isolated lipoproteins are added in an aqueous diluent to lyophilized human serum.
Inventors
- 👤 Gary J. Proksch
- 👤 Dean P. Bonderman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 21:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N2496/00; G01N2496/05; G01N33/92; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10T; Y10T436/00; Y10T436/10; Y10T436/104165; Y10T436/105831; Y10T436/106664; Y10T436/107497; Y10T436/109163
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
39 words
A substantially human serum for use as a standard or reference material is assaying human blood, said human serum being improved by containing in addition to essentially all normal human serum components, isolated non-primate, animal lipoproteins which contain cholesterol.
Claim 2
Dependent Claim
39 words
A substantially human serum for use as a standard or reference material is assaying human blood, said human serum being improved by containing in addition to essentially all normal human serum components, isolated non-primate, animal lipoproteins which contain cholesterol.
Claim 3
Dependent Claim
9 words
A lyophilized form of the serum of claim 1.
Claim 4
Dependent Claim
16 words
The serum of claim 1 in which the non-primate, animal lipoproteins comprise primarily high density lipoproteins.
Claim 5
Dependent Claim
34 words
The serum of claim 1 in which said human serum contains a sufficient amount of the animal lipoproteins to achieve a level of cholesterol in the substantially human serum of 4.0 grams per liter.
Claim 6
Dependent Claim
18 words
The serum of claim 1 in which the non-primate, animal lipoproteins comprise bovine lipoproteins obtained from bovine serum.
Claim 7
Dependent Claim
9 words
A lyophilized form of the serum of claim 5.
Claim 8
Dependent Claim
42 words
A substantially human serum for use as a standard or reference material in assaying human blood, said human serum being improved by containing in addition to essentially all normal human serum components, isolated non-primate, animal lipoproteins which contain mainly low density lipoproteins.
Claim 9
Dependent Claim
42 words
A substantially human serum for use as a standard or reference material in assaying human blood, said human serum being improved by containing in addition to essentially all normal human serum components, isolated non-primate, animal lipoproteins which contain mainly low density lipoproteins.
Claim 10
Dependent Claim
9 words
A lyophilized form of the serum of claim 7.
Claim 11
Dependent Claim
18 words
The serum of claim 5 in which the non-primate, animal lipoproteins comprise bovine lipoproteins obtained from bovine serum.
Claim 12
Dependent Claim
42 words
A lyophilized substantially human serum for use as a standard or reference material in assaying human blood, said human serum having its triglyceride level significantly reduced but containing high density lipoproteins and essentially all other serum components normally present in human serum.
Claim 13
Dependent Claim
42 words
A lyophilized substantially human serum for use as a standard or reference material in assaying human blood, said human serum having its triglyceride level significantly reduced but containing high density lipoproteins and essentially all other serum components normally present in human serum.
Claim 14
Dependent Claim
10 words
The serum of claim 10 which contains non-primate, animal lipoproteins.
Claim 15
Dependent Claim
18 words
The serum of claim 11 in which the non-primate, animal lipoproteins comprise bovine lipoproteins obtained from bovine serum.
Claim 16
Dependent Claim
29 words
A substantially human serum for use as a standard or reference material in assaying human blood, said human serum having normally present lipoproteins removed, but containing non-primate, animal lipoproteins.
Claim 17
Dependent Claim
29 words
A substantially human serum for use as a standard or reference material in assaying human blood, said human serum having normally present lipoproteins removed, but containing non-primate, animal lipoproteins.
Claim 18
Dependent Claim
9 words
A lyophilized form of the serum of claim 13.
Claim 19
Dependent Claim
18 words
The serum of claim 13 in which the non-primate animal lipoproteins comprise bovine lipoproteins obtained from bovine serum.
Claim 20
Independent Claim
28 words
A method of preparing a substantially human serum for use as a standard or reference material which comprises the addition of isolated high density lipoproteins to human serum.
Claim 21
Independent Claim
28 words
A method of preparing a substantially human serum for use as a standard or reference material which comprises the addition of isolated high density lipoproteins to human serum.
Claim 22
Dependent Claim
15 words
The method of claim 16 which additionally includes the step of lyophilizing the mixture produced.
Claim 23
Independent Claim
29 words
A method of preparing a substantially human serum for use as a standard or reference material which comprises the addition of isolated, high density lipoproteins to lyophilized human serum.
Claim 24
Independent Claim
29 words
A method of preparing a substantially human serum for use as a standard or reference material which comprises the addition of isolated, high density lipoproteins to lyophilized human serum.
Claim 25
Independent Claim
28 words
A method of preparing a substantially human serum for use as a standard or reference material which comprises the addition of non-primate, animal lipoproteins to lyophilized human serum.
Claim 26
Independent Claim
28 words
A method of preparing a substantially human serum for use as a standard or reference material which comprises the addition of non-primate, animal lipoproteins to lyophilized human serum.
Claim 27
Dependent Claim
18 words
The method of claim 19 in which the non-primate, animal lipoproteins comprise bovine lipoproteins obtained from bovine serum.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-29 | 1981-02-28 | 1981-08-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-29 | 1985-02-28 | 1985-08-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-29 | 1989-02-28 | 1989-08-30 | $7700.00 | Window Closed / Lapsed |