US3955925A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparation of optically clear serum
Assignee: Individual
•
Filing: 1975-06-13
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Granted: 1976-05-11
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Expires: 1995-06-13
Abstract
Abstract A stable optically clear serum is prepared for use in the preparation of standards and quality control reference material for the assay of human serum components. Prebeta lipoproteins, beta lipoproteins and chylomicrons are removed from human serum by specific precipitation. The serum resulting from the process disclosed retains its optical clarity upon freezing, lyophilization and reconstitution with aqueous media. Bovine lipoproteins are added to restore desired levels of cholesterol and triglycerides.
Inventors
- 👤 Gary J. Proksch
- 👤 Dean P. Bonderman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 02:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N2496/00; G01N2496/05; G01N33/92; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10T; Y10T436/00; Y10T436/10; Y10T436/103332; Y10T436/104165; Y10T436/104998; Y10T436/106664; Y10T436/107497; Y10T436/109163
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
100 words
A process for preparing a lyophilized serum standard or reference material for the assay of human blood comprising: a. mixing human serum with a metal cation selected from the group consisting of calcium, manganese and magnesium; b. adding to the serum containing said added metal cation a quantity of polysulfate sufficient to precipitate a lipoprotein-polysulfate complex said polysulfate being a polymer having a molecular weight of at least 10,000 and having at least one sulfate group per monomer; c. removing said lipoprotein-polysulfate complex precipitate from the serum; and d. lyophilizing the serum from which said lipoprotein-polysulfate complex has been removed.
Claim 2
Dependent Claim
100 words
A process for preparing a lyophilized serum standard or reference material for the assay of human blood comprising: a. mixing human serum with a metal cation selected from the group consisting of calcium, manganese and magnesium; b. adding to the serum containing said added metal cation a quantity of polysulfate sufficient to precipitate a lipoprotein-polysulfate complex said polysulfate being a polymer having a molecular weight of at least 10,000 and having at least one sulfate group per monomer; c. removing said lipoprotein-polysulfate complex precipitate from the serum; and d. lyophilizing the serum from which said lipoprotein-polysulfate complex has been removed.
Claim 3
Dependent Claim
12 words
The process of claim 1 in which said polysulfate is polyvinyl sulfate.
Claim 4
Dependent Claim
12 words
The process of claim 1 in which said polysulfate is dextran sulfate.
Claim 5
Dependent Claim
16 words
The process of claim 1 in which said serum is obtained from citrated whole human blood.
Claim 6
Dependent Claim
17 words
The process of claim 1 in which said serum is obtained from a plurality of human donors.
Claim 7
Dependent Claim
46 words
The process of claim 1 which additionally includes after removing said lipoprotein-polysulfate complex precipitate from the serum and prior to lyophilization of the serum: 1. dialyzing the serum and 2. adding back to the dialyzed serum several of the ingredients which were removed by the dialysis.
Claim 8
Dependent Claim
39 words
The process of claim 1 wherein said human serum is derived from plasma by a process comprising: a. adding topical bovine thrombin to the plasma, b. allowing the plasma to clot, and c. separating the serum from said clot.
Claim 9
Dependent Claim
12 words
The process of claim 1 in which said metal cation is magnesium.
Claim 10
Dependent Claim
41 words
The process of claim 8 which additionally includes after removing said lipoprotein-polysulfate complex precipitate from the serum, and prior to lyophilization of the serum: 1. adding a precipitating agent to the serum to precipitate said magnesium and 2. removing the precipitant.
Claim 11
Dependent Claim
38 words
The process of claim 1 which additionally includes after removing said lipoprotein-polysulfate complex precipitate from the serum and prior to lyophilization of the serum: the step of adding to the serum a lipoprotein fraction extracted from bovine serum.
Claim 12
Dependent Claim
59 words
The process of claim 10 in which said lipoprotein fraction extracted from bovine serum is obtained by: a. mixing bovine serum with a metal cation selected from the group consisting of: calcium, manganese, and magnesium b. adding to the mixture a quantity of polysulfate sufficient to precipitate a lipoprotein-polysulfate complex; and c. separating said complex from said bovine serum.
Claim 13
Dependent Claim
12 words
The process of claim 1 in which said metal cation is calcium.
Claim 14
Dependent Claim
41 words
The process of claim 12 which additionally includes after removing said lipoprotein-polysulfate complex precipitate from the serum, and prior to lyophilization of the serum: 1. adding a precipitating agent to the serum to precipitate said calcium and 2. removing the precipitant.
Claim 15
Dependent Claim
23 words
The process of claim 13 in which said precipitating agent is selected from the group consisting of: oxalate, phosphate, succinate, carbonate and citrate.
Claim 16
Dependent Claim
42 words
The process of claim 1 which additionally includes after removing said lipoprotein-polysuflate complex precipitate from the serum and prior to lyophilization of the serum; 1. adding a precipitating agent to the serum to precipitate said metal cation and 2. removing the precipitant.
Claim 17
Dependent Claim
23 words
The process of claim 15 in which said precipitating agent is selected from the group consisting of: oxalate, phosphate, succinate, carbonate and citrate.
Claim 18
Dependent Claim
12 words
The process of claim 16 in which said precipitating agent is oxalate.
Claim 19
Dependent Claim
45 words
A lyophilized human serum for use as a standard or reference material in assaying human blood, said lyophilized human serum having essentially all of its normally present pre-beta lipoproteins, beta lipoproteins and chylomicrons removed but containing essentially all alpha lipoproteins and other non-volatile serum components.
Claim 20
Dependent Claim
45 words
A lyophilized human serum for use as a standard or reference material in assaying human blood, said lyophilized human serum having essentially all of its normally present pre-beta lipoproteins, beta lipoproteins and chylomicrons removed but containing essentially all alpha lipoproteins and other non-volatile serum components.
Claim 21
Dependent Claim
17 words
The lyophilized serum of claim 18 wherein said lyophilized serum additionally includes lipoproteins extracted from bovine serum.
References Cited (Backward Citations)
US2824842A
Cited
US3260648A
Cited
US3274062A
Cited
US3497319A
Cited
US3682835A
Cited
US3764556A
Cited
US3770631A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-05-11 | 1979-11-10 | 1980-05-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-05-11 | 1983-11-10 | 1984-05-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-05-11 | 1987-11-10 | 1988-05-11 | $7700.00 | Window Closed / Lapsed |