Alcoholic precipitation and 1000x centrifugation preparation of hypercholesterolemic and hypertriglyceridemic sera as lipid determination control

Claim 1

A method in accordance with claim 1 comprising said isolating of cholesterol rich fraction from human plasma, comprising the steps of bringing frozen human plasma to room temperature, titrating said human plasma with 1.6-1.8 volume of about 0.025 M acetic acid until a pH of about 5.4 is reached, preparing a volume of absolute ethanol needed to make a mixture of about 85 percent (v/v) of titrated plasma and acetic acid and about 15 percent ethanol, bringing said titrated plasma and resultant prepared volume of absolute ethanol to a temperature of about 2*C. in an ice bath, adding said ethanol drop by drop while the said titrated plasma is stirred, keeping the temperature of the newly formed mixture below about 5*C., placing said mixture in prechilled centrifuge tubes at about -5*C. and precipitating protein for about one hour, centrifuging said tubes for about 30 minutes at about 1000 x g at about -5*C., decanting the resultant supernatant, the cold precipitate containing apProximately 75 percent of the cholesterol and only about 25 percent of the solids of the original plasma.