US4185964A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Lysing reagent
Assignee: Central Laboratories of Associated Maryland Pathologists Ltd
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Filing: 1977-02-08
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Granted: 1980-01-29
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Expires: 1997-02-08
Abstract
Abstract Azide-free blood diluents and cyanide ion-free erythrocyte lysing reagentshich are particularly advantageous for use with Coulter scanning devices. The lysing reagent comprises (1) a water-soluble quaternary ammonium salt of the formula R 1 N(R 2 ) 3 X wherein R 1 is a 12-20 C alkyl, each R 2 is the same or different and is lower alkyl or lower hydroxyalkyl and X is an anion, and (2) a minor amount of a water-soluble polycarboxylic acid.
Inventors
- 👤 Robert G. Lancaster
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 00:13
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N33/5005; G01N33/5094; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
102 words
A lysing reagent for lysing erythrocytes while leukocytes remain substantially intact and forming chromogens with hemoglobin comprising a cyanide ion-free aqueous solution containing a minor amount sufficient for erythrocytolysis and forming chromogens with hemoglobin of a water-soluble quaternary ammonium salt of the formula R.sub.1 N(R.sub.2).sub.3 X wherein R 1 is an alkyl having about 12 to 20 carbon atoms, each R 2 is the same or different and is lower alkyl or lower hydroxyalkyl, and X is an anion; and a minor amount sufficient to inhibit leukocytolysis of a water-soluble polycarboxylic acid, or salt thereof, of up to about 8 carbon atoms.
Claim 2
Dependent Claim
102 words
A lysing reagent for lysing erythrocytes while leukocytes remain substantially intact and forming chromogens with hemoglobin comprising a cyanide ion-free aqueous solution containing a minor amount sufficient for erythrocytolysis and forming chromogens with hemoglobin of a water-soluble quaternary ammonium salt of the formula R.sub.1 N(R.sub.2).sub.3 X wherein R 1 is an alkyl having about 12 to 20 carbon atoms, each R 2 is the same or different and is lower alkyl or lower hydroxyalkyl, and X is an anion; and a minor amount sufficient to inhibit leukocytolysis of a water-soluble polycarboxylic acid, or salt thereof, of up to about 8 carbon atoms.
Claim 3
Dependent Claim
26 words
The lysing reagent of claim 1 wherein the quaternary ammonium salt is selected from the group consisting of a halide, sulfate, phosphate, or a nitrate salt.
Claim 4
Dependent Claim
19 words
The lysing reagent of claim 2 wherein the polycarboxylic acid or salt is a hydroxy-containing polycarboxylic acid or salt.
Claim 5
Dependent Claim
15 words
The lysing reagent of claim 3 wherein the polycarboxylic salt is an alkali metal salt.
Claim 6
Dependent Claim
32 words
The lysing reagent of claim 3 wherein in the quaternary ammonium salt, R 1 has about 14 to 18 carbon atoms and R 2 is alkyl of 1 to 3 carbon atoms.
Claim 7
Dependent Claim
17 words
The lysing reagent of claim 3 wherein the reagent further comprises a minor amount of lower alkanol.
Claim 8
Dependent Claim
15 words
The lysing agent of claim 6 wherein the polycarboxylic acid salt is sodium potassium tartrate.
Claim 9
Dependent Claim
15 words
The lysing reagent of claim 7 wherein the quaternary ammonium salt is cetyltrimethyl ammonium bromide.
Claim 10
Dependent Claim
33 words
The lysing reagent of claim 7 wherein in the quaternary ammonium salt, R 1 has about 14 to 18 carbon atoms and R 2 is alkyl of 1 to about 3 carbon atoms.
Claim 11
Dependent Claim
40 words
The lysing reagent of claim 9 comprising about 10 to 50 grams per liter cetyltrimethyl ammonium bromide; about 5 to 50 grams per liter sodium potassium tartrate; and about 5 to 100 milliliters of ethanol per liter of lysing reagent.
Claim 12
Dependent Claim
15 words
The lysing reagent of claim 10 wherein the quaternary ammonium salt is cetyltrimethyl ammonium bromide.
Claim 13
Dependent Claim
46 words
In a method for lysing erythrocytes and forming chromogens with hemoglobin while leukocytes remain substantially intact wherein a reagent is reacted with a blood sample for erythrocytolysis and for forming chromogens with hemoglobin, the improvement employing as the lysing reagent the lysing reagent of claim 1.
Claim 14
Dependent Claim
14 words
The method of claim 12 wherein the polycarboxylic salt comprises an alkali metal salt.
Claim 15
Dependent Claim
31 words
The method of claim 13 wherein in the quaternary ammonium salt, R 1 has about 14 to 18 carbon atoms and R 2 is alkyl of 1 to 3 carbon atoms.
Claim 16
Dependent Claim
25 words
The method of claim 12 wherein the quaternary ammonium salt is selected from the group consisting of a halide, sulfate, phosphate, or a nitrate salt.
Claim 17
Dependent Claim
31 words
The method of claim 15 wherein in the quaternary ammonium salt, R 1 has about 14 to 18 carbon atoms and R 2 is alkyl of 1 to 3 carbon atoms.
Claim 18
Dependent Claim
18 words
The method of claim 15 wherein the polycarboxylic acid or salt is a hydroxy-containing polycarboxylic acid or salt.
Claim 19
Dependent Claim
13 words
The method of claim 17 wherein the polycarboxylic salt is sodium potassium tartrate.
Claim 20
Dependent Claim
39 words
The method of claim 18 comprising about 10 to 50 grams per liter cetyltrimethyl ammonium bromide; about 5 to 50 grams per liter sodium potassium tartrate; and about 5 to 100 milliliters of ethanol per liter of lysing reagent.
Claim 21
Dependent Claim
16 words
The method of claim 15 wherein the reagent further comprises a minor amount of lower alkanol.
Claim 22
Dependent Claim
14 words
The method of claim 20 wherein the quaternary ammonium salt is cetyltrimethyl ammonium bromide.
Claim 23
Dependent Claim
14 words
The method of claim 21 wherein the quaternary ammonium salt is cetyltrimethyl ammonium bromide.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-01-28 | 1983-07-30 | 1984-01-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-01-28 | 1987-07-30 | 1988-01-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-01-28 | 1991-07-30 | 1992-01-29 | $7700.00 | Window Closed / Lapsed |