US4405719A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of stabilizing platelets for determining multiple platelet parameters in reference control and calibrator compositions; diluents therefor; and combination stabilization procedures
Assignee: Coulter Electronics Inc
•
Filing: 1981-05-29
•
Granted: 1983-09-20
•
Expires: 2001-05-29
Abstract
Abstract This invention utilizes for stabilization of platelets a combination of iodoacetamide and an iminodiacetic acid or salt thereof, together with a compatible bacteriostatic agent, in an aqueous electrolytic solution which is maintained at a preselected range of pH and osmolality. In a preferred formulation, the stabilizing solution contains iodoacetamide, N-(2-acetamido)iminodiacetic acid and sodium penicillin. Ethylenediaminetetraacetic acid, or salts thereof, are optionally present as an additional ingredient.
Inventors
- 👤 Harold R. Crews
- 👤 II James H. Carter
- 👤 Ted Sena
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 12:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; G01N2496/00; G01N2496/05; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
117 words
A method for long term stabilization of blood platelets without the use of aldehydes or other cross-linking fixative agents for use in determining multiple platelet parameters in reference controls for electronic instrumentation, which method comprises adding to platelets in a suspending fluid a stabilizing solution which is an aqueous solution of: (1) iodoacetamide in a concentration of approximately 0.5 to 2.0 g per liter; and (2) one or more compounds selected from the group consisting of an iminodiacetic acid in a concentration of approximately 0.5 to 2.0 g per liter, and an equivalent weight of an alkali metal or alkaline buffer salt thereof, wherein the resultant media is maintained at a preselected range of pH and osmolality.
Claim 2
Independent Claim
117 words
A method for long term stabilization of blood platelets without the use of aldehydes or other cross-linking fixative agents for use in determining multiple platelet parameters in reference controls for electronic instrumentation, which method comprises adding to platelets in a suspending fluid a stabilizing solution which is an aqueous solution of: (1) iodoacetamide in a concentration of approximately 0.5 to 2.0 g per liter; and (2) one or more compounds selected from the group consisting of an iminodiacetic acid in a concentration of approximately 0.5 to 2.0 g per liter, and an equivalent weight of an alkali metal or alkaline buffer salt thereof, wherein the resultant media is maintained at a preselected range of pH and osmolality.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said iminodiacetic acid is N-(2-acetamido)iminodiacetic acid.
Claim 4
Dependent Claim
40 words
The method of claim 1 wherein a bacteriostatic agent which is a member of the penicillin family consisting essentially of penicillin G, potassium penicillin G, procaine penicillin G, penicillin V potassium, ampicillin and carbenicillin is present as an additional ingredient.
Claim 5
Dependent Claim
20 words
The method of claim 1 wherein ethylenediaminetetraacetic acid, the sodium or potassium salts thereof, is present as an additional ingredient.
Claim 6
Dependent Claim
22 words
The method of claim 4 wherein said ethylenediaminetetraacetic acid is present in a concentration of 0.5 g to 2.0 g per liter.
Claim 7
Dependent Claim
18 words
The method of claim 1 wherein said stabilizing solution is added directly to freshly collected platelet rich plasma.
Claim 8
Dependent Claim
22 words
The method of claim 1 wherein said stabilizing solution is added to platelet rich plasma for procurement of platelet concentrate by centrifugation.
Claim 9
Dependent Claim
13 words
The method of claim 1 wherein the final pH is adjusted to 7.0±0.5.
Claim 10
Dependent Claim
16 words
The method of claim 1 wherein the osmolality is adjusted to 330±30 milliosmoles using sodium chloride.
Claim 11
Dependent Claim
97 words
A hematology reference control media for determining multiple platelet parameters utilizing electronic instrumentation comprising a suspension of platelets which is stabilized by adding a stabilizing solution which is an aqueous solution of: (1) iodoacetamide in a concentration of approximately 0.5 to 2.0 g per liter. (2) one or more compounds selected from the group consisting of an iminodiacetic acid in a concentration of approximately 0.5 to 2.0 g per liter, and an equivalent weight of an alkali metal or alkaline buffer salt thereof, wherein the resultant media is maintained at a preselected range of pH and osmolality.
Claim 12
Dependent Claim
97 words
A hematology reference control media for determining multiple platelet parameters utilizing electronic instrumentation comprising a suspension of platelets which is stabilized by adding a stabilizing solution which is an aqueous solution of: (1) iodoacetamide in a concentration of approximately 0.5 to 2.0 g per liter. (2) one or more compounds selected from the group consisting of an iminodiacetic acid in a concentration of approximately 0.5 to 2.0 g per liter, and an equivalent weight of an alkali metal or alkaline buffer salt thereof, wherein the resultant media is maintained at a preselected range of pH and osmolality.
Claim 13
Dependent Claim
132 words
A multiple analysis hematology reference control comprising a suspension of stabilized red blood cells, together with platelets stabilized without the use of aldehydes or other fixing agents by adding to said platelets in a suspending fluid a stabilizing solution which is an aqueous solution of: (1) iodoacetamide in a concentration of approximately 0.5 to 2.0 g per liter. (2) one or more compounds selected from the group consisting of an iminodiacetic acid in a concentration of approximately 0.5 to 2.0 g per liter, and an equivalent weight of an alkali metal or alkaline buffer salt thereof, wherein the resultant media is maintained at a preselected range of pH and osmolality; wherein said control is capable of being used for testing the accuracy and precision of whole blood controls and multiple platelet parameters.
Claim 14
Dependent Claim
132 words
A multiple analysis hematology reference control comprising a suspension of stabilized red blood cells, together with platelets stabilized without the use of aldehydes or other fixing agents by adding to said platelets in a suspending fluid a stabilizing solution which is an aqueous solution of: (1) iodoacetamide in a concentration of approximately 0.5 to 2.0 g per liter. (2) one or more compounds selected from the group consisting of an iminodiacetic acid in a concentration of approximately 0.5 to 2.0 g per liter, and an equivalent weight of an alkali metal or alkaline buffer salt thereof, wherein the resultant media is maintained at a preselected range of pH and osmolality; wherein said control is capable of being used for testing the accuracy and precision of whole blood controls and multiple platelet parameters.
References Cited (Backward Citations)
US3968248A
Cited
US3873467A
Cited
US4102810A
Cited
US4157383A
Cited
US4116635A
Cited
US4160644A
Cited
US4198206A
Cited
US4302355A
Cited
US4213876A
Cited
US4299726A
Cited
US4324686A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-09-19 | 1987-03-21 | 1987-09-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-09-19 | 1991-03-21 | 1991-09-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-09-19 | 1995-03-21 | 1995-09-20 | $7700.00 | Window Closed / Lapsed |