US4992372A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for removing impurities from peroxidase solutions
Assignee: Enzymol International Inc
•
Filing: 1989-02-02
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Granted: 1991-02-12
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Expires: 2009-02-02
Abstract
Abstract A method for purifying an aqueous peroxidase enzyme containing solution and peroxidase solution purified thereby are disclosed; the method includes the steps of: providing an aqueous solution containing a peroxidase enzyme; adding a water-immiscible or partially water-miscible organic solvent to said aqueous solution to form a mixture having an organic phase and an aqueous phase; agitating said mixture to extract impurities from said aqueous phase into said organic phase; separating said aqueous phase from said organic phase; and discarding said organic phase.
Inventors
- 👤 Alexander R. Pokora
- 👤 Mary M. Stanley
- 👤 William L. Cyrus, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 19:45
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0065; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
82 words
A process for removing impurities from an aqueous peroxidase enzyme containing solution comprising the steps of: providing an aqueous solution containing a peroxidase enzyme; adding a water-immiscible or partially watermiscible organic solvent to said aqueous solution to form a mixture having an organic phase and an aqueous phase; agitating said mixture to extract impurities from said aqueous phase into said organic phase; separating said aqueous phase from said organic phase; discarding said organic phase and recovering said enzyme from said aqueous phase.
Claim 2
Dependent Claim
82 words
A process for removing impurities from an aqueous peroxidase enzyme containing solution comprising the steps of: providing an aqueous solution containing a peroxidase enzyme; adding a water-immiscible or partially watermiscible organic solvent to said aqueous solution to form a mixture having an organic phase and an aqueous phase; agitating said mixture to extract impurities from said aqueous phase into said organic phase; separating said aqueous phase from said organic phase; discarding said organic phase and recovering said enzyme from said aqueous phase.
Claim 3
Dependent Claim
12 words
The process of claim 1 wherein said peroxidase enzyme is horseradish peroxidase.
Claim 4
Dependent Claim
30 words
The process according to claim 2 wherein the amount of said solvent added to said aqueous solution is approximately 30 to 500 parts per 50 parts of said aqueous solution.
Claim 5
Dependent Claim
22 words
The process according to claim 2 wherein said solvent is selected from the group consisting of n-hexane, ethyl acetate, methylisobutylketone and dichloroethane.
Claim 6
Dependent Claim
25 words
The process according to claim 4 wherein said adding and agitating steps are performed at a temperature between about 5° C. and about 40° C.
Claim 7
Dependent Claim
21 words
The process according to claim 4 wherein said mixture is maintained at a pH ranging from about 4 to about 12.
Claim 8
Dependent Claim
14 words
The process according to claim 4 wherein said agitating step comprise stirring said mixture.
Claim 9
Dependent Claim
15 words
The process according to claim 7 wherein said separating step comprises centrifuging said agitated mixture.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-02-11 | 1994-08-13 | 1995-02-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-02-11 | 1998-08-13 | 1999-02-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-02-11 | 2002-08-13 | 2003-02-12 | $7700.00 | Window Closed / Lapsed |