US4707447A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fungal chloroperoxidase method
Assignee: Novartis Vaccines and Diagnostics Inc
•
Filing: 1983-05-24
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Granted: 1987-11-17
•
Expires: 2003-05-24
Abstract
Abstract A halogenation method using a haloperoxidase obtained from a fungus selected from the dematiaceous hyphomycetes. The enzyme has an optimum activity above about pH 5.0, and can oxidize chloride, bromide, or iodide ions.
Inventors
- 👤 Jennie C. Hunter
- 👤 Angela Belt
- 👤 Lynn S. Sotos
- 👤 Michelle E. Fonda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 21:00
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0065; C12P; C12P3/00; C12P7/00; C12P7/02; C12P7/04; C12P7/18
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
127 words
A method of oxidizxing a halide selected from the group consisting of chloride, bromide, and iodide, at a selected pH between about 4 and 9, said method comprising: providing an enzxyme which is derived from a fungus selected from the group consisting of the genera Alternaria, Curvularia, Drechslera, Ulocladium, Dendryphiella, Dendrylphion therefore Dichotomophthora and Embellisia in the enteroblastic tretic group or from a dmeatiaaceous hyphomycete fungus selected from the group consisting of the genera Botrytis, Bispora, Cladosporium, Humicola, Stemplyllium, Wardomyces and Epicoccum in the acroauxic holoblastic group, and which is capable of chlorinating monochlorodimedon in the presence of H 2 O 2 and chloride ions, at pH 7.0, and reacting the halide with the enzyme in the presence of H 2 O 2 at the selected pH.
Claim 2
Independent Claim
127 words
A method of oxidizxing a halide selected from the group consisting of chloride, bromide, and iodide, at a selected pH between about 4 and 9, said method comprising: providing an enzxyme which is derived from a fungus selected from the group consisting of the genera Alternaria, Curvularia, Drechslera, Ulocladium, Dendryphiella, Dendrylphion therefore Dichotomophthora and Embellisia in the enteroblastic tretic group or from a dmeatiaaceous hyphomycete fungus selected from the group consisting of the genera Botrytis, Bispora, Cladosporium, Humicola, Stemplyllium, Wardomyces and Epicoccum in the acroauxic holoblastic group, and which is capable of chlorinating monochlorodimedon in the presence of H 2 O 2 and chloride ions, at pH 7.0, and reacting the halide with the enzyme in the presence of H 2 O 2 at the selected pH.
Claim 3
Dependent Claim
10 words
The method of claim 1, wherein said halide includes chloride.
Claim 4
Dependent Claim
10 words
The method of claim 1, wherein the halide includes bromide.
Claim 5
Dependent Claim
10 words
The method of claim 1, wherein the halide includes iodide.
Claim 6
Dependent Claim
19 words
The method of claim 1, wherein said reacting is performed at a selected pH between about 6 and 8.
Claim 7
Dependent Claim
19 words
The method of claim 1, wherein said reacting is performed at a selected pH between about 5 and 6.
Claim 8
Dependent Claim
222 words
The method of claim 1, wherein the fungus is selected from one of the following organisms: ______________________________________
CMCC NRRL
GENUS/SPECIE # OTHER # # GROUP
______________________________________
Alternaria alternata
1286 15150 5
Alternaria alternata
1288 15291 5
Alternaria alternata
0514 15289 5
Alternaria sp.
1287 15193 5
Curvularia 0850 ATCC 38580 5
cymbopogonis
Curvularia inaequalis
1262 ATCC 14992 5
Curvularia inaequalis
0755 15147 5
Curvularia tuberculata
0842 ATCC 15058 5
Curvularia 0844 QM 8326 5
verruciformis
Curvularia lunata
0843 ATCC 12017 5
Curvularia lunata
1296 15293 5
Curvularia lunata
1297 15196 5
Dichotomophthora
1288 CBS 239.48 5
portulacae
Drechslera halodes
1299 15151 5
Drechslera sp.
0834 15290 5
Drechslera sp.
0835 15146 5
Drechslera sp.
1302 15294 5
Drechslera sp.
1290 15197 5
Ulocladium sp.
0282 15200 5
Ulocladium sp.
1291 15201 5
Ulocladium sp.
1292 15102 5
Ulocladium sp.
1300 15103 5
Ulocladium sp.
1301 15104 5
Botrytis cinerea
0311 15327 4
Botrytis sp. 0253 15326 4
Botrytis sp. 0256 15309 4
Botrytis sp. 1293 15292 4
Dendryphiella salina
1282 CBS 141.60 5
Dendryphion nanum
1263 CBS 263.70 5
Ulocladium chartarum
1289 15149 5
Bispora betulina
0245 15140 4
Cladosporium sp.
0812 ATCC 20251 4
Cladosporium sp.
1294 15195 4
Humicola fuscoatra
0397 15198 4
Stemphylium 1285 ATCC 20090 4
macrosporidium
Wardomyces anomalus
0363 15144 4
Embellisia allii
1305 15148 5
______________________________________
Claim 9
Dependent Claim
173 words
The method of claim 7, wherein the fungus is selected from one of the following organisms. ______________________________________
CMCC NRRL
GENUS/SPECIE # OTHER # # GROUP
______________________________________
Alternaria alternata
1286 15150 5
Alternaria alternata
1288 15291 5
Alternaria alternata
0514 15289 5
Curvularia 0850 ATCC 38580 5
cymbopogonis
Curvularia inaequalis
1262 ATCC 14992 5
Curvularia inaequalis
0755 15147 5
Curvularia lunata
0843 ATCC 12017 5
Curvularia tuberculata
0842 ATCC 15058 5
Curvularia 0844 QM 8326 5
verruciformis
Curvularia lunata
1296 15293 5
Curvularia lunata
1297 15196 5
Dichotomophthora
1288 CBS 239.48 5
portulacae
Drechslera halodes
1299 15151 5
Drechslera sp.
0834 15290 5
Drechslera sp.
0835 15146 5
Drechslera sp.
1302 15294 5
Drechslera sp.
1290 15197 5
Ulocladium sp.
0282 15200 5
Ulocladium sp.
1291 15201 5
Ulocladium sp.
1292 15102 5
Ulocladium sp.
1300 15103 5
Ulocladium sp.
1301 15104 5
Botrytis cinerea
0311 15327 4
Botrytis sp. 0253 15326 4
Botrytis sp. 0256 15309 4
Botrytis sp. 1293 15292 4
Epicoccum nigrum
1268 15143 4
Embellisia allii
1305 15148 5
______________________________________
Claim 10
Dependent Claim
13 words
The method of claim 8, wherein the fungus includes C. inaequalis, NRRL #15147.
Claim 11
Dependent Claim
13 words
The method of claim 8, wherein the fungus includes Drechslera sp. NRRL #15146.
Claim 12
Dependent Claim
30 words
The method of claim 1, wherein said providing includes isolating from a soluble fraction of fungal growth material, an enzyme which precipitates between about 40% and 55% saturated ammonium sulfate.
Claim 13
Dependent Claim
42 words
The method of claim 1, wherein the enzxyme is provided in undisrupted cells of the selected fungus, and said reacting includes immobilizing the undisrupted cells on a solid support and reacting the immobilized cells with the compound under the selected reaction conditions.
Claim 14
Dependent Claim
36 words
The method of claim 1 further including before said reacting step, providing a compound to be halogenated, selected from one of the group consistging of alkenes, alkynes, cyclopropanes, beta-keto acids, cyclic beta-diketone and aromatic ring compounds.
Claim 15
Dependent Claim
14 words
The method of claim 13, wherein the compound to be halogenated includes an alkene.
Claim 16
Independent Claim
129 words
A method of oxidizing iodide ions to molecular iodine, at a selected pH between about 4.0 and 9.0, in the presence of H 2 O 2 , said method comprising: producing an enyzme which is derived from a dematiaceous hyphomycetes fungus selected from the group consisting of the genera Alternaria, Curvularia, Drechslera, Ulocladium, Dendryphiella, Dendryphion and Dichotomophthora in the enteroblastic tretic group or from a dematiaceous hyphomycete fungus selected from the group consisting of the genera Botryts, Bispora, Cladosporium, Humicola, Stemphyllium, Wardomyces and Epicoccum in the acroauxtic holoblastic group, and which is capable of chlorinating monochlorodimedon in the presence of hydrogen peroxide and chlorine ions, at pH 7.0, and reacting the enzyme with an iodide salt in the presence of H 2 O 2 , at the selected pH.
Claim 17
Independent Claim
129 words
A method of oxidizing iodide ions to molecular iodine, at a selected pH between about 4.0 and 9.0, in the presence of H 2 O 2 , said method comprising: producing an enyzme which is derived from a dematiaceous hyphomycetes fungus selected from the group consisting of the genera Alternaria, Curvularia, Drechslera, Ulocladium, Dendryphiella, Dendryphion and Dichotomophthora in the enteroblastic tretic group or from a dematiaceous hyphomycete fungus selected from the group consisting of the genera Botryts, Bispora, Cladosporium, Humicola, Stemphyllium, Wardomyces and Epicoccum in the acroauxtic holoblastic group, and which is capable of chlorinating monochlorodimedon in the presence of hydrogen peroxide and chlorine ions, at pH 7.0, and reacting the enzyme with an iodide salt in the presence of H 2 O 2 , at the selected pH.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-16 | 1991-05-18 | 1991-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-16 | 1995-05-18 | 1995-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-16 | 1999-05-18 | 1999-11-17 | $7700.00 | Window Closed / Lapsed |