US4331761A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stabilization of peroxidase
Assignee: Akzona Inc
•
Filing: 1980-01-04
•
Granted: 1982-05-25
•
Expires: 2000-01-04
Abstract
Abstract New and useful stable peroxidase compositions are disclosed which are particularly useful as reagents in enzyme immunoassay (EIA) tests. The novel peroxidase compositions contain polyvalent ions of groups 3 and 4 of the Periodic Table. These novel compositions are stable even in low peroxidase concentrations, and are relatively invulnerable to freeze-drying. Preferably, the polyvalent ions selected are those of Al, Zn, Mg, Fe, and Cu.
Inventors
- 👤 Edward C. Dawson
- 👤 Jan D. H. Homan
- 👤 Bauke K. Van Weemen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 10:46
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; A; A61; A61K; A61K38/00; A61K38/16; A61K38/43; A61K38/44; C12N; C12N9/00; C12N9/96; C12Y; C12Y111/00; C12Y111/01; C12Y111/01017; Y; Y10; Y10S; Y10S435/00; Y10S435/963
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
125 words
A method of manufacturing a composition useful as a diagnostic tool for enzyme immunoassay, comprising: (a) freezing at a temperature from about -40 Deg. F. to about -50 Deg. F. an aqueous peroxidase composition containing (1) peroxidase in an amount from about 1 nanogram per milliliter to about 25 micrograms per milliliter, and (2) at least one polyvalent metal salt selected from the group consisting of the sulphate, the phosphate, the halide, and nitrate of any of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Ar, Zn, Ga, and Al present in a peroxidase-stabilizing amount of from about 0.0001 M to about 0.05 M to form a frozen ice; and (b) subliming said frozen ice under reduced pressure to form a freeze-dried composition.
Claim 2
Independent Claim
125 words
A method of manufacturing a composition useful as a diagnostic tool for enzyme immunoassay, comprising: (a) freezing at a temperature from about -40 Deg. F. to about -50 Deg. F. an aqueous peroxidase composition containing (1) peroxidase in an amount from about 1 nanogram per milliliter to about 25 micrograms per milliliter, and (2) at least one polyvalent metal salt selected from the group consisting of the sulphate, the phosphate, the halide, and nitrate of any of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Ar, Zn, Ga, and Al present in a peroxidase-stabilizing amount of from about 0.0001 M to about 0.05 M to form a frozen ice; and (b) subliming said frozen ice under reduced pressure to form a freeze-dried composition.
Claim 3
Dependent Claim
16 words
The method according to claim 1, in which the aqueous composition further contains a sequestering agent.
Claim 4
Dependent Claim
37 words
The method according to claim 2, in which the sequestering agent is selected from the group consisting of ethylene diamine tetra-acetic acid (EDTA), citric acid, tartaric acid, glucuronic acid, saccharic acid, and suitable salts of these acids.
Claim 5
Dependent Claim
34 words
The method according to claim 1 or claim 2, in which the aqueous composition further contains one or more ingredients selected from the group consisting of a buffer, polyethylene glycol, albumin, and a sugar.
Claim 6
Dependent Claim
21 words
The method according to claim 4, in which the sugar is selected from the group consisting of sucrose, sorbitol and mannitol.
Claim 7
Dependent Claim
6 words
The freeze-dried composition of claim 1.
Claim 8
Independent Claim
127 words
A method of manufacturing a composition useful as a diagnostic tool for enzyme immunoassay, comprising: (a) freezing at a temperature from about -40 Deg. F. to about -50 Deg. F. an aqueous peroxidase composition consisting essentially of (1) peroxidase in an amount from about 1 nanogram per milliliter to about 25 micrograms per milliliter, and (2) at least one polyvalent metal salt selected from the group consisting of the sulphate, the phosphate, the halide, and nitrate of any of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Ar, Zn, Ga, and Al present in a peroxidase-stabilizing amount of from about 0.0001 M to about 0.05 M to form a frozen ice; and (b) subliming said frozen ice under reduced pressure to form a freeze-dried composition.
Claim 9
Independent Claim
127 words
A method of manufacturing a composition useful as a diagnostic tool for enzyme immunoassay, comprising: (a) freezing at a temperature from about -40 Deg. F. to about -50 Deg. F. an aqueous peroxidase composition consisting essentially of (1) peroxidase in an amount from about 1 nanogram per milliliter to about 25 micrograms per milliliter, and (2) at least one polyvalent metal salt selected from the group consisting of the sulphate, the phosphate, the halide, and nitrate of any of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Ar, Zn, Ga, and Al present in a peroxidase-stabilizing amount of from about 0.0001 M to about 0.05 M to form a frozen ice; and (b) subliming said frozen ice under reduced pressure to form a freeze-dried composition.
Claim 10
Dependent Claim
16 words
The method according to claim 7, in which the aqueous composition further contains a sequestering agent.
Claim 11
Dependent Claim
37 words
The method according to claim 8, in which the sequestering agent is selected from the group consisting of ethylene diamine tetra-acetic acid (EDTA), citric acid, tartaric acid, glucuronic acid, saccharic acid, and suitable salts of these acids.
Claim 12
Dependent Claim
33 words
The method according to claim 7 or claim 9, in which the aqueous composition further contains one or more ingredients selected from the group consisting of buffer, polyethylene glycol, albumin, and a sugar.
Claim 13
Dependent Claim
21 words
The method according to claim 10, in which the sugar is selected from the group selected from sucrose, sorbitol and mannitol.
Claim 14
Dependent Claim
6 words
The freeze-dried composition of claim 7.
References Cited (Backward Citations)
GB626848A
Cited
US3019171A
Cited
US3005714A
Cited
US2990338A
Cited
GB1069045A
Cited
US3367842A
Cited
US3413198A
Cited
GB1293227A
Cited
USRE29169E
Cited
US3953295A
Cited
US3912593A
Cited
US3947324A
Cited
US4169012A
Cited
US4228240A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-24 | 1985-11-23 | 1986-05-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-24 | 1989-11-23 | 1990-05-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-24 | 1993-11-23 | 1994-05-25 | $7700.00 | Window Closed / Lapsed |