Process for the demonstration and determination of reaction components having specific binding affinity for each other

Claim 2

Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a. providing a given quantity of the binding partner of the component to be determined, which binding partner is in an insoluble form; b. reacting the component to be determined with its binding partner to form a reaction mixture having a solid phase and a liquid phase; c. separating the solid phase from the liquid phase; d. adding to said solid phase a given quantity of a coupling product of the component to be determined with an enzyme; and e. determining the enzyme activity of the solid phase which is a measure of the quantity of reaction component to be determined.

Claim 3

Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a. providing a given quantity of the binding partner of the component to be determined in a soluble form; .Iadd. b. reacting the component to be determined with its binding partner; .Iaddend. .[.b..]. .Iadd.c. .Iaddend.adding a given quantity of an antibody against said binding partner, which antibody is in an insoluble form, to form a reaction mixture having a solid phase and a liquid phase; .[.c..]. .Iadd.d. .Iaddend.separating the solid phase from the liquid phase; .[.d..]. .Iadd.e. .Iaddend.adding to said solid phase a given quantity of a coupling product of the component to be determined with an enzyme; and .[.e..]. .Iadd.f. .Iaddend.determining the enzyme activity of either the liquid phase of the solid phase, which activity is a measure of the quantity of the reaction component to be determined.

Claim 4

Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a. providing a given quantity of the binding partner of the component to be determined in a soluble form; .Iadd. b. reacting the component to be determined with its binding partner; .Iaddend. .[.b..]. .Iadd.c. .Iaddend.adding a given quantity of an antibody against said binding partner, which antibody is in an insoluble form, to form a reaction mixture having a solid phase and a liquid phase; .[.c..]. .Iadd.d. .Iaddend.separating the solid phase from the liquid phase; .[.d..]. .Iadd.e. .Iaddend.adding to said solid phase a given quantity of a coupling product of the component to be determined with an enzyme; and .[.e..]. .Iadd.f. .Iaddend.determining the enzyme activity of either the liquid phase of the solid phase, which activity is a measure of the quantity of the reaction component to be determined.

Claim 5

The process of claim 1 in which the enzyme is an oxido-reductase.

Claim 6

The process of claim 2 in which the enzyme is an oxido-reductase. .Iadd. 5. Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a. providing a given quantity of the binding partner of the component to be determined, which binding partner is in an insoluble form; b. reacting the component to be determined with its binding partner to form a reaction mixture having a solid phase and a liquid phase; c. separating the solid phase from the liquid phase; d. adding to said solid phase a given quantity of a coupling product obtained by binding a substance capable of reacting specifically with one of the reaction components, to an enzyme; and e. determining the enzyme activity of the solid phase which is a measure of

Claim 7

the quantity of reaction component to be determined. .Iaddend. .Iadd. 6. Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a. providing a given quantity of the binding partner of the component to be determined in a soluble form; b. reacting the component to be determined with its binding partner; c. adding a given quantity of antibody against said binding partner, which antibody is in an insoluble form, to form a reaction mixture having a solid phase and a liquid phase; d. separating the solid phase from the liquid phase; e. adding to said solid phase a given quantity of a coupling product obtained by binding a substance capable of reacting specifically with one of the reaction components, to an enzyme; and f. determining the enzyme activity of either the liquid phase or the solid phase, which activity is a measure of the quantity of the reaction component to be determined..Iaddend.

Claim 8

the quantity of reaction component to be determined. .Iaddend. .Iadd. 6. Process for the demonstration and determination of a component of the reaction between a bindable substance selected from the group consisting of an antigen, a hapten, and a low molecular substance, and a protein capable of binding said bindable substance specifically, said protein being selected from the group consisting of an antibody and a specific binding protein, comprising the steps of: a. providing a given quantity of the binding partner of the component to be determined in a soluble form; b. reacting the component to be determined with its binding partner; c. adding a given quantity of antibody against said binding partner, which antibody is in an insoluble form, to form a reaction mixture having a solid phase and a liquid phase; d. separating the solid phase from the liquid phase; e. adding to said solid phase a given quantity of a coupling product obtained by binding a substance capable of reacting specifically with one of the reaction components, to an enzyme; and f. determining the enzyme activity of either the liquid phase or the solid phase, which activity is a measure of the quantity of the reaction component to be determined..Iaddend.