US4228240A
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US
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Stabilization of peroxidase
Assignee: Akzona Inc
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Filing: 1979-01-25
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Granted: 1980-10-14
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Expires: 1999-01-25
Abstract
Abstract New and useful stable peroxidase compositions are disclosed which are particularly useful as reagents in enzyme immunoassay (EIA) tests. The novel peroxidase compositions contain polyvalent ions of groups 3 and 4 of the Periodic Table. These novel compositions are stable even at low concentrations of peroxidase, and are relatively invulnerable to freeze-drying. Preferably, the polyvalent ions selected are those of Al, Zn, Mg, Fe, and Cu.
Inventors
- 👤 Edward C. Dawson
- 👤 Jan D. H. Homan
- 👤 Bauke K. Van Weemen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 13:01
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; A; A61; A61K; A61K38/00; A61K38/16; A61K38/43; A61K38/44; C12N; C12N9/00; C12N9/96; C12Y; C12Y111/00; C12Y111/01; C12Y111/01017; Y; Y10; Y10S; Y10S435/00; Y10S435/963
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
80 words
Process for the stabilization of peroxidase-containing compositions for use in enzyme immunoassay tests, consisting essentially of: adding one or more polyvalent metal ion(s) selected from the group consisting of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, and Al to an aqueous solution consisting essentially of peroxidase present from about 1 nanogram per milliliter to about 25 micrograms per milliliter, wherein the metal ion concentration is in an amount of at least about 0.0001 M.
Claim 2
Dependent Claim
80 words
Process for the stabilization of peroxidase-containing compositions for use in enzyme immunoassay tests, consisting essentially of: adding one or more polyvalent metal ion(s) selected from the group consisting of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, and Al to an aqueous solution consisting essentially of peroxidase present from about 1 nanogram per milliliter to about 25 micrograms per milliliter, wherein the metal ion concentration is in an amount of at least about 0.0001 M.
Claim 3
Dependent Claim
17 words
The process according to claim 1, wherein the polyvalent metal ion is derived from an iron salt.
Claim 4
Dependent Claim
14 words
The process according to claim 1, further consisting essentially of lyophilizing the peroxidase-containing composition.
Claim 5
Dependent Claim
16 words
The process according to claim 1, wherein a sequestering agent is also added to the composition.
Claim 6
Dependent Claim
67 words
An aqueous composition for use in enzyme immunoassay tests, consisting essentially of: a peroxidase present from about 1 nanogram per milliliter to 25 micrograms per milliliter; and one or more polyvalent metal ions selected from the group consisting of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, and Al, wherein the ion is present in an amount of at least 0.0001 M.
Claim 7
Dependent Claim
67 words
An aqueous composition for use in enzyme immunoassay tests, consisting essentially of: a peroxidase present from about 1 nanogram per milliliter to 25 micrograms per milliliter; and one or more polyvalent metal ions selected from the group consisting of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, and Al, wherein the ion is present in an amount of at least 0.0001 M.
Claim 8
Dependent Claim
15 words
The composition of claim 5 wherein the polyvalent ion is part of an iron salt.
Claim 9
Dependent Claim
15 words
The composition of claim 5 wherein the polyvalent ion is part of an aluminum salt.
Claim 10
Dependent Claim
12 words
The composition of claim 5 wherein the peroxidase containing composition is lyophilized.
Claim 11
Dependent Claim
15 words
The composition of claim 5 having a suitable sequestering agent for the polyvalent metal ions.
Claim 12
Dependent Claim
12 words
The composition of claim 5 wherein the peroxidase employed is horseradish peroxidase.
Claim 13
Dependent Claim
22 words
The composition of claim 5 wherein the polyvalent ions are present in an amount from about 0.0001 M to about 0.05 M.
Claim 14
Dependent Claim
25 words
The composition of claim 5 wherein the peroxidase is present in an amount from about 10 nanograms per milliliter to about 10 micrograms per milliliter.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-10-14 | 1984-04-14 | 1984-10-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-10-14 | 1988-04-14 | 1988-10-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-10-14 | 1992-04-14 | 1992-10-14 | $7700.00 | Window Closed / Lapsed |