US4657864A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for stabilizing peroxidase solutions
Assignee: WESTVACO Corp A CORP OF
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Filing: 1985-06-18
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Granted: 1987-04-14
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Expires: 2005-06-18
Abstract
Abstract Solutions containing peroxidase enzymes may be treated to increase their stability and shelf life by reducing their protease level and filtering through a microporous filter to remove any microbial contamination that might secrete additional protease enzymes.
Inventors
- 👤 David K. Lo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 08:54
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0065; Y; Y10; Y10S; Y10S435/00; Y10S435/814
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
72 words
Process for the stabilization of peroxidase containing solutions comprising: (a) preparing a peroxidase solution to achieve a protease activity of from about 0.02 to 0.5 U/mg dry weight; (b) filtering the solution through a membrane having a pore size in the range of from about 0.20 to 0.45 μm to produce a sterilized solution; and, (c) storing the sterilized solution in sterile containers at a temperature of between about 10-30 degrees C.
Claim 2
Dependent Claim
72 words
Process for the stabilization of peroxidase containing solutions comprising: (a) preparing a peroxidase solution to achieve a protease activity of from about 0.02 to 0.5 U/mg dry weight; (b) filtering the solution through a membrane having a pore size in the range of from about 0.20 to 0.45 μm to produce a sterilized solution; and, (c) storing the sterilized solution in sterile containers at a temperature of between about 10-30 degrees C.
Claim 3
Dependent Claim
67 words
Process for the stabilization of peroxidase containing solutions comprising: (a) treating a peroxidase solution to achieve a protease level less than about 0.5 U/mg dry weight; (b) filtering the treated solution through a membrane having a pore size of about 0.22 μm to produce a sterilized solution; and, (c) storing the sterilized and treated solution in a sterile container at a temperature of about 25 degrees C.
Claim 4
Dependent Claim
67 words
Process for the stabilization of peroxidase containing solutions comprising: (a) treating a peroxidase solution to achieve a protease level less than about 0.5 U/mg dry weight; (b) filtering the treated solution through a membrane having a pore size of about 0.22 μm to produce a sterilized solution; and, (c) storing the sterilized and treated solution in a sterile container at a temperature of about 25 degrees C.
References Cited (Backward Citations)
US3947324A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-04-13 | 1990-10-13 | 1991-04-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-13 | 1994-10-13 | 1995-04-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-04-13 | 1998-10-13 | 1999-04-14 | $7700.00 | Window Closed / Lapsed |