US4764468A
Expired / Lapsed
US
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Process for stabilizing the activity of peroxidase in solution
Assignee: Roche Diagnostics GmbH
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Filing: 1986-03-04
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Granted: 1988-08-16
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Expires: 2006-03-04
Abstract
Abstract The present invention provides a process for stabilizing the activity of peroxidase in solution by the addition of a specific activity stabilizer, wherein, to the enzyme present in solid or dissolved form, there is added, as activity stabilizer, phenol which optionally contains one or more substituents selected from lower alkyl radicals and chlorine and bromine atoms, in an amount of from 0.0005 to 2% by weight, referred to the solution.
Inventors
- 👤 Rainer Wehner
- 👤 Johann Mattersberger
- 👤 Dagmar Klenner
- 👤 Gerlinde Deutsch-Weyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 08:38
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12N; C12N9/00; C12N9/96
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
88 words
A process for stabilizing the activity of peroxidase enzyme in solution by the addition of a specific activity stabilizer comprising adding to the enzyme in solid or dissolved form, as the activity stabilizer, phenol which is unsubstituted or is substituted with one or more substituents selected from the group consisting of C 1 -C 3 lower alkyl, chlorine and bromine, in an amount of form 0.0005 to 2% by weight, referred to the solution and wherein the peroxidase is used as a conjugate with an immunologically active substance.
Claim 2
Dependent Claim
88 words
A process for stabilizing the activity of peroxidase enzyme in solution by the addition of a specific activity stabilizer comprising adding to the enzyme in solid or dissolved form, as the activity stabilizer, phenol which is unsubstituted or is substituted with one or more substituents selected from the group consisting of C 1 -C 3 lower alkyl, chlorine and bromine, in an amount of form 0.0005 to 2% by weight, referred to the solution and wherein the peroxidase is used as a conjugate with an immunologically active substance.
Claim 3
Dependent Claim
15 words
The process of claim 1, wherein 0.01 to 0.05% by weight of phenol are added.
Claim 4
Dependent Claim
15 words
The process of claim 1 further comprising, after the phenol is added, lyophilizing the solution.
Claim 5
Dependent Claim
31 words
The process of claim 1, wherein the enzyme is in the form of a lyophilizate which has been reconstituted in aqueous solvent and the phenol is added to the reconstituted lyophilisate.
Claim 6
Dependent Claim
14 words
The process of claim 1 wherein the peroxidase is conjugated with an immunologically-active substance.
Claim 7
Dependent Claim
21 words
The process of claim 5, wherein the immunologically-active substance is an antibody or a fragment thereof, an antigen or a hapten.
Claim 8
Dependent Claim
23 words
The process of claim 6, wherein the immunologically-active substance is digoxin, digoxigenin, triiodothyronine or thyroxin or an antibody against TSH, ferritin or AFP.
Claim 9
Dependent Claim
48 words
A stabilized peroxidase enzyme preparation comprising peroxidase and 0.0005 to 2% by weight of phenol or phenol substituted with one or more substituents selected from the group consisting of C 1 -C 3 lower alkyl, chlorine and bromine, wherein the peroxidase is conjugated with an immunologically active substance.
Claim 10
Dependent Claim
48 words
A stabilized peroxidase enzyme preparation comprising peroxidase and 0.0005 to 2% by weight of phenol or phenol substituted with one or more substituents selected from the group consisting of C 1 -C 3 lower alkyl, chlorine and bromine, wherein the peroxidase is conjugated with an immunologically active substance.
Claim 11
Dependent Claim
24 words
The stabilized peroxidase enzyme preparation of claim 8 wherein the immunologically active substance is an antibody or fragment thereof, an antigen or a hapten.
Claim 12
Dependent Claim
26 words
The stabilized peroxidase enzyme preparation of claim 8 wherein the immunologically active substance is digoxin, digoxigenin, triiodothyronine, thyroxin or an antibody against TSH, ferritin or AFP.
Claim 13
Dependent Claim
12 words
The stabilized peroxidase enzyme preparation of claim 8 wherein phenol is used.
Claim 14
Dependent Claim
17 words
The stabilized peroxidase enzyme preparation of claim 8 wherein phenol substituted one or two times is used.
Claim 15
Dependent Claim
17 words
The stabilized peroxidase enzyme preparation of claim 12 wherein bromophenol, chlorophenol, dichlorophenol, dibromophenol or cresol is used.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-16 | 1992-02-15 | 1992-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-16 | 1996-02-15 | 1996-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-16 | 2000-02-15 | 2000-08-16 | $7700.00 | Window Closed / Lapsed |