US4874692A
Expired / Lapsed
US
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Binder composition and analytical element having stabilized peroxidase in layer containing the composition
Assignee: Eastman Kodak Co
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Filing: 1987-07-20
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Granted: 1989-10-17
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Expires: 2007-07-20
Abstract
Abstract An analytical element has a peroxidase-labeled ligand analog distributed within a layer comprising from 0.1 to 10.0 g/m 2 of poly(vinyl-alcohol) and from 0.2 to 20.0 g/m 2 of glycerol. The concentration of the glycerol must be greater than 1 times the concentration of poly(vinylalcohol) in the layer. As a result, the peroxidase retains more of its stability prior to use. Such elements can be used to determine a number of different immunologically reactive analytes, such as digoxin.
Inventors
- 👤 Jon N. Eikenberry
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/52; G01N33/525; G01N33/526; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/826
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
56 words
An analytical element comprising an absorbent carrier material containing a peroxidase-labeled ligand analog for an immunologically reactive ligand uniformly distributed in a layer comprising from 0.1 to 10.0 g/m 2 of poly(vinylalcohol) and from 0.2 to 20 g/m 2 of glycerol; provided that the glycerol concentration must be greater than 1.0 times the concentration of poly(vinylalcohol).
Claim 2
Dependent Claim
56 words
An analytical element comprising an absorbent carrier material containing a peroxidase-labeled ligand analog for an immunologically reactive ligand uniformly distributed in a layer comprising from 0.1 to 10.0 g/m 2 of poly(vinylalcohol) and from 0.2 to 20 g/m 2 of glycerol; provided that the glycerol concentration must be greater than 1.0 times the concentration of poly(vinylalcohol).
Claim 3
Dependent Claim
23 words
The element of claim 1 wherein the concentration of glycerol in the layer is from 2 to 4 times the concentration of poly(vinylalcohol).
Claim 4
Dependent Claim
32 words
The element of claim 1 or 2, further comprising an interactive composition which reacts with the peroxidase-labeled ligand analog to provide a spectrophotometric signal in the presence of a substrate for peroxidase.
Claim 5
Dependent Claim
18 words
The element of claim 3, wherein the interactive composition comprises α-glycerol phosphate oxidase and a triarylimidazole leuco dye.
Claim 6
Dependent Claim
15 words
The element of claim 4 further comprising an immobilized receptor for the immunologically reactive ligand.
Claim 7
Dependent Claim
98 words
A multilayer analytical element comprising a nonporous support having thereon, in order, a registration layer; a water-soluble layer containing a peroxidase-labeled ligand analog for an immunologically reactive ligand uniformly distributed in from 0.1 to 10.0 g/m 2 of poly(vinylalcohol) and from 0.2 to 20 g/m 2 of glycerol; provided that the glycerol concentration in the layer is greater than 1.0 times the concentration of poly(vinlyalcohol); a porous spreading layer; and, in one or more layers, an interactive composition which is reacts with the ligand analog to provide a spectrophotometric signal in the presence of a substrate for peroxidase.
Claim 8
Dependent Claim
98 words
A multilayer analytical element comprising a nonporous support having thereon, in order, a registration layer; a water-soluble layer containing a peroxidase-labeled ligand analog for an immunologically reactive ligand uniformly distributed in from 0.1 to 10.0 g/m 2 of poly(vinylalcohol) and from 0.2 to 20 g/m 2 of glycerol; provided that the glycerol concentration in the layer is greater than 1.0 times the concentration of poly(vinlyalcohol); a porous spreading layer; and, in one or more layers, an interactive composition which is reacts with the ligand analog to provide a spectrophotometric signal in the presence of a substrate for peroxidase.
Claim 9
Dependent Claim
23 words
The element of claim 6, wherein the concentration of glycerol in the layer is from 1.0 to 1.5 times the concentration of poly(vinylalcohol).
Claim 10
Dependent Claim
15 words
The element of claim 6 or 7, wherein the peroxidase-labeled ligand analog is peroxidase-labeled digoxin.
Claim 11
Dependent Claim
20 words
The element of claim 6 or 7 wherein the interactive composition comprises α-glycerol phosphate oxidase and a triarylimidazole leuco dye.
Claim 12
Dependent Claim
21 words
The element of claim 6 or 7 further comprising an immobilized receptor for the immunologically reactive ligand in the spreading layer.
Claim 13
Dependent Claim
26 words
The element of claim 6 or 7 wherein said peroxidase-labeled ligand analog is present in a concentration of at least about 10 -6 g/m 2 .
Claim 14
Independent Claim
115 words
A method for the determination of an immunologically reactive ligand comprising the steps of: A. in the presence of a receptor for said ligand, contacting a sample of a liquid suspected of containing the ligand with an analytical element comprising an absorbent carrier material having a water-soluble layer containing a peroxidase-labeled ligand analog for the ligand uniformly distributed in from 0.1 to 10.0 g/m 2 of poly(vinylalcohol) and from 0.2 to 20 g/m 2 of glycerol; provided that the concentration of the glycerol in the layer is greater than 1.0 times the concentration of poly(vinylalcohol); and B. determining the amount of the ligand as a result of the presence of complexed or uncomplexed ligand analog.
Claim 15
Independent Claim
115 words
A method for the determination of an immunologically reactive ligand comprising the steps of: A. in the presence of a receptor for said ligand, contacting a sample of a liquid suspected of containing the ligand with an analytical element comprising an absorbent carrier material having a water-soluble layer containing a peroxidase-labeled ligand analog for the ligand uniformly distributed in from 0.1 to 10.0 g/m 2 of poly(vinylalcohol) and from 0.2 to 20 g/m 2 of glycerol; provided that the concentration of the glycerol in the layer is greater than 1.0 times the concentration of poly(vinylalcohol); and B. determining the amount of the ligand as a result of the presence of complexed or uncomplexed ligand analog.
Claim 16
Dependent Claim
14 words
The method of claim 12 wherein the element contains immobilized receptor for said ligand.
Claim 17
Dependent Claim
40 words
The method of claim 12 wherein the liquid sample is contacted with the element in such a manner that the receptor-ligand analog complex is immobilized in the element and horizontal separation of uncomplexed ligand analog from immobilized complex is effected.
Claim 18
Dependent Claim
18 words
The method of claim 14 wherein separation is accomplished with a wash step subsequent to said contacting step.
Claim 19
Dependent Claim
74 words
The method of claim 12, 13, 14 or 15, wherein the element comprises a nonporous support having thereon, in order, a registration layer, a layer containing the peroxidase-labeled ligand analog uniformly distributed in from 0.1 to 10 g/m 2 of poly(vinylalcohol) and from 0.2 to 20 g/m 2 of glycerol; provided that the concentration of the glycerol in the layer is greater than 1.0 times the concentration of poly(vinylalcohol), and a porous spreading layer.
Claim 20
Dependent Claim
27 words
The method of claim 12, 13, 14 or 15, wherein the immunological reactive ligand is digoxin in a biological fluid wherein the ligand analog is peroxidase-labeled digoxin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-10-16 | 1993-04-17 | 1993-10-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-10-16 | 1997-04-17 | 1997-10-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-10-16 | 2001-04-17 | 2001-10-17 | $7700.00 | Window Closed / Lapsed |