Patent US4399030A successfully refreshed from Google Patents.
US4734360A
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Colorimetric ethanol analysis method and test device
Assignee: LifeScan Inc
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Filing: 1984-06-26
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Granted: 1988-03-29
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Expires: 2004-06-26
Abstract
Abstract A disposable test strip device for detecting and measuring ethanol in aqueous solutions is disclosed. The test strip includes an inert support pad that contains a stabilized dry form of the enzyme alcohol oxidase, a material having peroxidative activity and an oxygen acceptor that reacts with hydrogen peroxide to give a compound of changed color. The use of this strip to determine ethanol levels colorimetrically is also disclosed.
Inventors
- 👤 Roger C. Phillips
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:47
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
118 words
A method for determining the concentration of ethanol in the bloodstream of a human who is suspected of having consumed ethanol, which comprises: (a) reacting saliva of said human with a dry, room-temperature-stable test device comprising an inert support pad containing alcohol oxidase, mannitol present in an amount sufficient to stabilize the alcohol oxidase, a salt that buffers in the range of pH 6-9, a substance having peroxidase activity, and a substance which will react with hydrogen peroxide to give a compound of changed color, whereby a change of color is obtained in the presence of ethanol; and (b) comparing any color so obtained to a standard to determine the ethanol concentration in the bloodstream of said human.
Claim 2
Independent Claim
118 words
A method for determining the concentration of ethanol in the bloodstream of a human who is suspected of having consumed ethanol, which comprises: (a) reacting saliva of said human with a dry, room-temperature-stable test device comprising an inert support pad containing alcohol oxidase, mannitol present in an amount sufficient to stabilize the alcohol oxidase, a salt that buffers in the range of pH 6-9, a substance having peroxidase activity, and a substance which will react with hydrogen peroxide to give a compound of changed color, whereby a change of color is obtained in the presence of ethanol; and (b) comparing any color so obtained to a standard to determine the ethanol concentration in the bloodstream of said human.
Claim 3
Dependent Claim
17 words
The method of claim 1, wherein said test device further comprises a polyamine polyacid metal chelating agent.
Claim 4
Dependent Claim
29 words
The method of claim 1, wherein said test device comprises alcohol oxidase in intimate admixture with from 300-2500 mg of said mannitol per 1000 enzyme units of alcohol oxidase.
Claim 5
Dependent Claim
28 words
The method of claim 3, wherein said test device further comprises from 2-50 mg of a polyamine polyacid metal chelating agent per 1000 enzyme units of alcohol oxidase.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-03-29 | 1991-09-28 | 1992-03-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-03-29 | 1995-09-28 | 1996-03-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-03-29 | 1999-09-28 | 2000-03-29 | $7700.00 | Window Closed / Lapsed |