US4642286A
Expired / Lapsed
US
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Composition and method for ethanol determination
Assignee: Individual
•
Filing: 1984-05-07
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Granted: 1987-02-10
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Expires: 2004-05-07
Abstract
Abstract A novel composition and method is disclosed for detecting or quantitatively determining the level of ethanol. This is a colorimetric method and analysis can be done by the visual method. The composition comprises an enzyme alcohol oxidase, a peroxide detecting system consisting of peroxidase and chemicals and chemicals to stabilize alcohol oxidase.
Inventors
- 👤 Mervin J. Moldowan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 00:18
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/98
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
96 words
A method for determining the level of ethanol in a fluid or air comprising exposing absorbent material to said fluid or air, the absorbent material being impregnated with a composition comprising: (a) An indicator solution comprising orthotolidine, a yellow dye, ethanol and tris(hydroxymethy)-aminomethane/sodium phosphate buffer solution and (b) an enzyme solution comprising dextran, sodium chloride, peroxidase, alcohol oxidase, semicarbazide hydrochloride and a tris(hydroxymethyl)-aminomethane/sodium phosphate buffer solution said dextran and sodium chloride being present in amounts sufficient to stabilize the alcohol oxidase and the semicarbazide hydrchloride being present in an amount sufficient to inhibit said alcohol oxidase.
Claim 2
Independent Claim
96 words
A method for determining the level of ethanol in a fluid or air comprising exposing absorbent material to said fluid or air, the absorbent material being impregnated with a composition comprising: (a) An indicator solution comprising orthotolidine, a yellow dye, ethanol and tris(hydroxymethy)-aminomethane/sodium phosphate buffer solution and (b) an enzyme solution comprising dextran, sodium chloride, peroxidase, alcohol oxidase, semicarbazide hydrochloride and a tris(hydroxymethyl)-aminomethane/sodium phosphate buffer solution said dextran and sodium chloride being present in amounts sufficient to stabilize the alcohol oxidase and the semicarbazide hydrchloride being present in an amount sufficient to inhibit said alcohol oxidase.
Claim 3
Dependent Claim
92 words
The method of claim 1 wherein the indicator solution comprises: Ortho-tolidine: 0.002% to 20% by weight dye: 0.001% to 50% by weight ethanol: 0.5% to 95% by weight buffer solution (pH 5 to 10): 0.01% to 70% by weight and the enzyme solution comprises: dextran: 0.02% to 50% by weight sodium chloride: 0.02% to 20% by weight peroxidase (95 units/mg): 0.001% to 20% to weight alcohol oxidase (35 units/mg)*: 0.001% to 40% by weight semicarbazide hydrochloride: 0.02% to 3% by weight buffer solution (pH 5 to 10): 0.01% to 75% by weight
Claim 4
Dependent Claim
68 words
The method of claim 1 wherein said indicator solution comprises 0.1 g ortho-tolidine, 0.042 g FDC yellow No. 5 dye, 2 ml ethanol (95% v/v) and 23 ml buffer solution of pH 8.0 and said enzyme solution comprises 232 units peroxidase, 140 units alcohol oxidase, 0.15 g dextran, 0.018 g sodium chloride, 0.0123 g semicarbazide hydrochloride in a total volume of 2 ml buffer solution at pH 8.0.
Claim 5
Dependent Claim
13 words
The method of claim 3 wherein dextran has a molecular weight of 40,000.
Claim 6
Dependent Claim
54 words
A composition of making ethanol detection strips comprising dextran, sodium chloride, peroxidase, alcohol oxidase, semicarbazide, a buffer solution and a hydrogen peroxide detecting reagent said dextran and sodium chloride being present in amounts sufficient to stabilize the alcohol oxidase and the semicarbazide hydrochloride being present in an amount sufficient to inhibit said alcohol oxidase.
Claim 7
Dependent Claim
54 words
A composition of making ethanol detection strips comprising dextran, sodium chloride, peroxidase, alcohol oxidase, semicarbazide, a buffer solution and a hydrogen peroxide detecting reagent said dextran and sodium chloride being present in amounts sufficient to stabilize the alcohol oxidase and the semicarbazide hydrochloride being present in an amount sufficient to inhibit said alcohol oxidase.
Claim 8
Dependent Claim
62 words
The composition of claim 5 wherein the components are present in the indicated amounts: dextran: 0.02% to 50% by weight sodium chloride: 0.02% to 20% by weight peroxidase (95 units/mg): 0.001% to 20% by weight alcohol oxidase (35 units/mg)*: 0.0001% to 40% by weight semicarbazide hydrochloride: 0.02% to 3% by weight buffer solution (pH 5 to 10): 0.01% to 75% by weight
Claim 9
Dependent Claim
36 words
The composition of claim 5 comprising 232 units peroxidase, 140 units alcohol oxidase, 0.15 g dextran, 0.018 g sodium chloride, 0.0123 g semicarbazide hydrochloride in a total volume of 2 ml buffer solution at pH 8.0.
Claim 10
Dependent Claim
14 words
A composition of claim 7 wherein said dextran has a molecular weight of 40,000.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-09 | 1990-08-11 | 1991-02-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-09 | 1994-08-11 | 1995-02-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-09 | 1998-08-11 | 1999-02-10 | $7700.00 | Window Closed / Lapsed |