US4757016A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for stabilizing the activity of peroxidase in solution
Assignee: Roche Diagnostics GmbH
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Filing: 1986-03-04
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Granted: 1988-07-12
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Expires: 2006-03-04
Abstract
Abstract The present invention provides a process for stabilizing the activity of peroxidase in solution by the addition of a specific activity stabilizer, wherein, to the enzyme present in solid or dissolved form, when the peroxidase is used as a conjugate with an immunologically effective substance, there is added, as activity stabilizer, aminopyrine in an amount of from 0.0005 to 2% by weight, referred to the solution.
Inventors
- 👤 Dagmar Klenner
- 👤 Gerd Kleinhammer
- 👤 Rolf Deeg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 15:54
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/96; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
60 words
A process for stabilizing the activity of peroxidase enzyme in solution wherein the peroxidase is used as a conjugate with an immunologically effective substance by the addition of a specific stabilizer comprising adding to the enzyme in solid or dissolved form as the activity stabilizer, aminopyrine in an amount of from 0.0005 to 2% by weight, referred to the solution.
Claim 2
Dependent Claim
60 words
A process for stabilizing the activity of peroxidase enzyme in solution wherein the peroxidase is used as a conjugate with an immunologically effective substance by the addition of a specific stabilizer comprising adding to the enzyme in solid or dissolved form as the activity stabilizer, aminopyrine in an amount of from 0.0005 to 2% by weight, referred to the solution.
Claim 3
Dependent Claim
15 words
The process of claim 1, wherein 0.005 to 1% by weight of aminopyrine are added.
Claim 4
Dependent Claim
16 words
The process of claim 1 further comprising, after the aminopyrine is added, lyophilizing the enzyme solution.
Claim 5
Dependent Claim
31 words
The process of claim 1, wherein the enzyme is in the form of a lyophilizate which has been reconstituted in aqueous solvent and the aminopyrine is added to the reconstituted lyophilisate.
Claim 6
Dependent Claim
21 words
The process of claim 1, wherein the immunologically-effective substance is an antibody or a fragment thereof, an antigen or a hapten.
Claim 7
Dependent Claim
22 words
The process of claim 5, wherein the immunologically-effective substance is digoxin, digoxigenin, or thyroxin or an antibody against TSH, ferritin or AFP.
Claim 8
Dependent Claim
17 words
The process of claim 5, wherein the immunologically-effective substance is an antibody against AFP, ferritin or TSH.
Claim 9
Dependent Claim
11 words
The process of claim 1 further comprising adding a preserving agent.
Claim 10
Dependent Claim
29 words
A composition comprising a stabilized peroxidase enzyme preparation wherein the peroxidase is conjugated with an immunologically effective substance peroxidase together with 0.005 to 2% by weight of amino pyrine.
Claim 11
Dependent Claim
29 words
A composition comprising a stabilized peroxidase enzyme preparation wherein the peroxidase is conjugated with an immunologically effective substance peroxidase together with 0.005 to 2% by weight of amino pyrine.
Claim 12
Dependent Claim
25 words
The process of claim 5 wherein the immunologically-effective substance is TBG, cortisol, vitamin B12, or an antibody against hCG, LH, FSH, prolactin, CEA or insulin.
Claim 13
Dependent Claim
18 words
The stabilized peroxidase enzyme preparation of claim 9 wherein 0.005 to 1% by weight of aminopyrine is used.
Claim 14
Dependent Claim
23 words
The stabilized peroxidase enzyme preparation of claim 11 wherein the immunologically-effective substance is an antibody or fragment thereof, an antigen or a hapten.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-07-12 | 1992-01-11 | 1992-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-07-12 | 1996-01-11 | 1996-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-07-12 | 2000-01-11 | 2000-07-12 | $7700.00 | Window Closed / Lapsed |