US4350762A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Aminopyrine improved Trinder's reagent and dosing process for hydrogen peroxide from enzymatic oxidation of metabolic substrata with the same
Assignee: Elvi SpA
•
Filing: 1981-02-03
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Granted: 1982-09-21
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Expires: 2001-02-03
Abstract
Abstract An aminopyrine-improved Trinder's reagent is disclosed to be used for the dosing of hydrogen peroxide from enzymatic oxidation of metabolic substrata, such as glucose, cholesterol, uric acid, tryglycerides and coline. The use of such an improved reagent in Trinder's reaction enables to avoid any interference from bilirubin, particularly in the determination of uric acid. The dosing process (for uric acid) is also disclosed by means of such a reagent.
Inventors
- 👤 Ugo De Luca
- 👤 Francesco Zoppi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 17:45
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/62; C12Q1/26; C12Q1/28; C12Q1/54; C12Q1/60; C12Q2326/00; C12Q2326/90; C12Q2326/96
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
53 words
In a Trinder's reagent for the clinical analysis of hydrogen peroxide by the enzymatic oxidation of a metabolic substrate in the presence of bilirubin, said reagent containing 2,4-dichlorophenyl sulphonate and 4-aminophenazone; the improvement in which the Trinder's reagent contains 1 to 30 g/l of aminopyrine to block the interferring action of said bilirubin.
Claim 2
Dependent Claim
53 words
In a Trinder's reagent for the clinical analysis of hydrogen peroxide by the enzymatic oxidation of a metabolic substrate in the presence of bilirubin, said reagent containing 2,4-dichlorophenyl sulphonate and 4-aminophenazone; the improvement in which the Trinder's reagent contains 1 to 30 g/l of aminopyrine to block the interferring action of said bilirubin.
Claim 3
Dependent Claim
20 words
A reagent as claimed in claim 1, in which said aminopyrine is present in the amount of about 15 g/l.
Claim 4
Dependent Claim
57 words
A reagent as claimed in claim 1, containing peroxidase, about 2,000 I.U./l, 2,4-dichlorophenyl sulphonate-sodium salt about 1 g/l, monobasic potassium phosphate (KH 2 PO 4 ) about 18 g/l, sodium tetraborate (Na 2 B 4 O 7 .10 H 2 O) about 13.9 g/l, 4-aminophenazone about 260 mg/l, aminopyrine about 15 g/l and uricase about 100 I.U./l.
References Cited (Backward Citations)
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Cited
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Cited
US4295853A
Cited
US4226713A
Cited
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Cited
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Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-09-20 | 1986-03-22 | 1986-09-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-09-20 | 1990-03-22 | 1990-09-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-09-20 | 1994-03-22 | 1994-09-21 | $7700.00 | Window Closed / Lapsed |