US4251629A
Expired / Lapsed
US
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Determination of hydrogen peroxide
Assignee: Fujifilm Wako Pure Chemical Corp
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Filing: 1978-07-28
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Granted: 1981-02-17
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Expires: 1998-07-28
Abstract
Abstract Colorimetrical determination of hydrogen peroxide in clinical examinations, food additives analyses and general analyses can be carried out accurately, rapidly and economically by using a composition for producing color producing reagents comprising 4-aminoantipyrine, a N-substituted-3-alkylaniline and a hydrogen peroxide activating agent such as peroxidase, said composition forming a coloring material having excellent sensitivity and color stability in contact with hydrogen peroxide.
Inventors
- 👤 Kazuhiko Yamanisi
- 👤 Kuniaki Tokuda
- 👤 Tosiro Hanada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:03
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/62; C09; C09B; C09B53/00; C09B53/02; C12Q1/26; C12Q1/28; C12Q1/54; C12Q1/60; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; C12Q2326/00; C12Q2326/90; C12Q2326/96; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
98 words
A composition for producing color producing reagents for determining hydrogen peroxide which comprises 4-aminoantipyrine, a N-substituted-3-alkylaniline of the formula: ##STR4## wherein R 1 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to 5 carbon atoms; R 2 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to 5 carbon atoms and R 3 is methyl, ethyl or propyl, and an agent for activating hydrogen peroxide.
Claim 2
Dependent Claim
98 words
A composition for producing color producing reagents for determining hydrogen peroxide which comprises 4-aminoantipyrine, a N-substituted-3-alkylaniline of the formula: ##STR4## wherein R 1 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to 5 carbon atoms; R 2 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to 5 carbon atoms and R 3 is methyl, ethyl or propyl, and an agent for activating hydrogen peroxide.
Claim 3
Dependent Claim
37 words
A composition according to claim 1, wherein in the formula (I) R 1 is a lower alkyl group having 1 to 5 carbon atoms and R 2 is a hydroxyalkyl group having 1 to 5 carbon atoms.
Claim 4
Dependent Claim
29 words
A composition according to claim 1, wherein in the formula (I) R 1 and R 2 are the same or different alkyl groups having 1 to 5 carbon atoms.
Claim 5
Dependent Claim
29 words
A composition according to claim 1, wherein in the formula (I) R 1 and R 2 are the same or different hydroxyalkyl groups having 1 to 5 carbon atoms.
Claim 6
Dependent Claim
11 words
A composition according to claim 1, wherein the N-substituted-3-alkylaniline is N-ethyl-N-hydroxyethyl-3-methylaniline.
Claim 7
Dependent Claim
11 words
A composition according to claim 1, wherein the N-substituted-3-alkylaniline is N,N-dimethyl-3-methylaniline.
Claim 8
Dependent Claim
11 words
A composition according to claim 1, wherein the N-substituted-3-alkylaniline is N,N-bis(β-hydroxyethyl)-3-ethylaniline.
Claim 9
Dependent Claim
15 words
A composition according to claim 1, wherein the agent for activating hydrogen peroxide is peroxidase.
Claim 10
Dependent Claim
40 words
A composition according to claim 1, wherein the agent for activating hydrogen peroxide activating agent is a mixture of an iodide and one compound selected from the group consisting of tungstic acid and its salts, molybdic acid and its salts.
Claim 11
Dependent Claim
129 words
In a method for determining hydrogen peroxide in an enzymatic reaction system wherein 4-aminoantipyrine is oxidatively condensed with an aniline to produce a color developed material and the optical absorbance of the color developed material is determined, the improvement comprising employing an N-substituted-3-alkylaniline of the formula: ##STR5## wherein R 1 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to B 5 carbon atoms; R 2 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to 5 carbon atoms and R 3 is methyl, ethyl or propyl, in the presence of an agent for activating hydrogen peroxide.
Claim 12
Dependent Claim
129 words
In a method for determining hydrogen peroxide in an enzymatic reaction system wherein 4-aminoantipyrine is oxidatively condensed with an aniline to produce a color developed material and the optical absorbance of the color developed material is determined, the improvement comprising employing an N-substituted-3-alkylaniline of the formula: ##STR5## wherein R 1 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to B 5 carbon atoms; R 2 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to 5 carbon atoms and R 3 is methyl, ethyl or propyl, in the presence of an agent for activating hydrogen peroxide.
References Cited (Backward Citations)
US3886045A
Cited
US3979262A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-02-17 | 1984-08-18 | 1985-02-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-02-17 | 1988-08-18 | 1989-02-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-02-17 | 1992-08-18 | 1993-02-17 | $7700.00 | Window Closed / Lapsed |