US4291121A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Bilirubin-resistant determination of uric acid and cholesterol
Assignee: Bayer Corp
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Filing: 1980-03-24
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Granted: 1981-09-22
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Expires: 2000-03-24
Abstract
Abstract A composition, test device, method of making a test device and process for bilirubin-resistant determination of an analyte selected from uric acid or cholesterol in a fluid sample are disclosed. More particularly, the composition is of the type comprising means responsive to the presence of the particular analyte to be determined in the sample, a phenol and 4-aminophenazone to which resistance to interference by bilirubin is imparted by inclusion therein of reagent means comprising a ferrocyanide ion. The uric acid or cholesterol responsive means is preferably of the type which determines peroxides formed from enzymatic conversion of the analyte. The composition can optionally be incorporated with a carrier to provide a test device.
Inventors
- 👤 Giancarlo Acquati
- 👤 Giovanni Berti
- 👤 Piero Fossati
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 19:09
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; C; C12; C12Q; C12Q1/00; C12Q1/60; C12Q1/62; G01N33/52; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10T; Y10T436/00; Y10T436/14; Y10T436/145555; Y10T436/146666; Y10T436/147777; Y10T436/148888
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
48 words
Composition for the determination of an analyte selected from uric acid and cholesterol in a fluid sample of the type comprising means responsive to the presence of said analyte in said sample, a phenol or napthol and 4-aminophenazone wherein the improvement comprises reagent means comprising a ferrocyanide ion.
Claim 2
Dependent Claim
48 words
Composition for the determination of an analyte selected from uric acid and cholesterol in a fluid sample of the type comprising means responsive to the presence of said analyte in said sample, a phenol or napthol and 4-aminophenazone wherein the improvement comprises reagent means comprising a ferrocyanide ion.
Claim 3
Dependent Claim
12 words
The composition of claim 1 wherein the phenol is an unsubstituted phenol.
Claim 4
Dependent Claim
11 words
The composition of claim 1 wherein the phenol is 3,5-dichloro-2-hydroxybenzenesulfonic acid.
Claim 5
Dependent Claim
14 words
The composition of claim 1 wherein the reagent means is a ferrocyanide ion salt.
Claim 6
Dependent Claim
13 words
The composition of claim 4 wherein the ferrocyanide ion salt is sodium ferrocyanide.
Claim 7
Dependent Claim
13 words
The composition of claim 4 wherein the ferrocyanide ion salt is potassium ferrocyanide.
Claim 8
Dependent Claim
11 words
The composition of claim 1 wherein the analyte is uric acid.
Claim 9
Dependent Claim
40 words
A composition for the determination of uric acid in a fluid sample of the type comprising means responsive to the presence of uric acid on the sample, unsubstituted phenol and 4-aminophenazone wherein the improvement comprises reagent means comprising potassium ferrocyanide.
Claim 10
Dependent Claim
40 words
A composition for the determination of uric acid in a fluid sample of the type comprising means responsive to the presence of uric acid on the sample, unsubstituted phenol and 4-aminophenazone wherein the improvement comprises reagent means comprising potassium ferrocyanide.
Claim 11
Dependent Claim
10 words
The composition of claim 1 wherein the analyte is cholesterol.
Claim 12
Dependent Claim
38 words
A composition for the determination of cholesterol in a fluid sample of the type comprising means responsive to the presence of cholesterol in the sample, unsubstituted phenol and 4-aminophenazone wherein the improvement comprises reagent means comprising sodium ferrocyanide.
Claim 13
Dependent Claim
38 words
A composition for the determination of cholesterol in a fluid sample of the type comprising means responsive to the presence of cholesterol in the sample, unsubstituted phenol and 4-aminophenazone wherein the improvement comprises reagent means comprising sodium ferrocyanide.
Claim 14
Dependent Claim
14 words
A test device which comprises a carrier incorporated with the composition of claim 1.
Claim 15
Dependent Claim
18 words
A method of making a test device which comprises incorporating a carrier with the composition of claim 1.
Claim 16
Dependent Claim
31 words
The method of claim 12 wherein the composition is incorporated with the carrier by impregnating the carrier with a solution of said test means, followed by drying of the impregnated carrier.
Claim 17
Dependent Claim
28 words
A process for determination of uric acid in a fluid sample which comprises contacting said sample with the composition of claim 1 and observing any resultant color formed.
Claim 18
Dependent Claim
30 words
A process for determination of uric acid in a fluid sample which comprises contacting said sample with the test device of claim 11 and observing any resultant color formed thereon.
Claim 19
Dependent Claim
27 words
A process for determination of cholesterol in a fluid sample which comprises contacting said sample with the composition of claim 1 and observing any resultant color formed.
Claim 20
Dependent Claim
29 words
A process for determination of cholesterol in a fluid sample which comprises contacting said sample with the test device of claim 11 and observing any resultant color formed thereon.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-21 | 1985-03-23 | 1985-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-21 | 1989-03-23 | 1989-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-21 | 1993-03-23 | 1993-09-22 | $7700.00 | Window Closed / Lapsed |