US4831126A
Expired / Lapsed
US
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Antigenic polysaccharide specific to Brucella abortus and Yersinia enterocolitica serotype 0:9
Assignee: HER MAJESTY IN RIGHT OF CANADA AGRICULTURE, Minister of
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Filing: 1985-02-26
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Granted: 1989-05-16
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Expires: 2005-02-26
Abstract
Abstract The antigenic O-chain polysaccharides from Brucella abortus and Yersinia enterocolitica serotype 0:9 have similar structure, i.e. 1-2 linked 4,6-dideoxy-4-formamido- alpha -D-mannopyranosyl units. The antigen is more readily isolated from the Yersinia and may be chemically modified to have a hydrophobic moiety attached thereto. The antigen can be adsorbed or coupled to carriers suitable for immunoassay use such as ELISA and RIA. A monoclonal antibody capable of specifically binding to B. abortus and Y. enterocolitica, has been produced. Assay procedures and kits therefor, are described.
Inventors
- 👤 David R. Bundle
- 👤 Malcolm B. Perry
- 👤 John W. Cherwonogrodzky
- 👤 J. Robert Duncan
- 👤 Martine G. Caroff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 20:59
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/02; A61K39/098; A61K39/025; C; C07; C07K; C07K16/00; C07K16/12; C07K16/1203; C07K16/1221; C07K16/1228; C12; C12P; C12P19/00; C12P19/04; A61K2039/51; A61K2039/52; A61K2039/521; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/23; G01N2333/24; G01N2400/00; G01N2400/10; G01N2400/50; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
29 words
Antigenic polysaccharide specific to Brucella abortus and Yersinia enterocolitica serotype 0:9 comprising about 96 to 100 1-2 linked 4,6-dideoxy-4-formamido-α-D-mannopyranosyl units and having a molecular weight about 15,000 to 18,000.
Claim 2
Dependent Claim
29 words
Antigenic polysaccharide specific to Brucella abortus and Yersinia enterocolitica serotype 0:9 comprising about 96 to 100 1-2 linked 4,6-dideoxy-4-formamido-α-D-mannopyranosyl units and having a molecular weight about 15,000 to 18,000.
Claim 3
Dependent Claim
20 words
The antigenic polysaccharide of claim 1 having about 100 of said mannopyranosyl units and a molecular weight of about 18,000.
Claim 4
Dependent Claim
14 words
The antigenic polysaccharide of claim 1 modified to have a hydrophobic moiety attached thereto.
Claim 5
Dependent Claim
23 words
The antigenic polysaccharide of claim 3 wherein said hydrophobic moiety is a fatty acid ester linked to the polysaccharide via the aliphatic chain.
Claim 6
Dependent Claim
21 words
The antigenic polysaccharide of claim 4 wherein said moiety is --NH--(CH.sub.2).sub.n COOR where R is a lower alkyl group and n=7-17.
Claim 7
Dependent Claim
15 words
The antigenic polysaccharide of claim 5 wherein said moiety has the formula: --NH--(CH.sub.2).sub.11 COOCH.sub.2 CH.sub.3.
Claim 8
Dependent Claim
14 words
The antigenic polysaccharide of claim 1, adsorbed onto or coupled to a hydrophobic carrier.
Claim 9
Dependent Claim
14 words
The antigenic polysaccharide of claim 7 wherein the carrier is selected from hydrophobic polymers.
Claim 10
Dependent Claim
14 words
The antigenic polysaccharide-carrier combination of claim 8 wherein the carrier is an ELISA plate.
Claim 11
Dependent Claim
68 words
A method of preparing the antigenic polysaccharide of claim 1 suitable for detecting Brucella abortus or antibodies thereto, comprising: (a) growing Yersinia enterocolitica serotype 0:9 in a suitable growth medium, and recovering cells thereof, (b) isolating phenol-soluble lipopolysaccharide (LPS) from the cells, (c) cleaving the O-antigenic polysaccharide from the LPS with mild acid hydrolysis and removing the lipid portion, and (d) recovering the antigenic polysaccharide of claim 1.
Claim 12
Dependent Claim
107 words
A method of preparing the antigenic polysaccharide of claim 3 linked to a hydrophobic moiety suitable for detecting Brucella abortus or antibodies thereto, comprising: (a) growing Yersinia enterocolitica serotype 0:9 in a suitable growth medium, and recovering cells thereof, (b) isolating phenol-soluble lipopolysaccharide (LPS) from the cells, (c) cleaving the O-antigenic polysaccharide from the LPS with mild acid hydrolysis and removing the lipid portion, (d) modifying O-antigenic polysaccharide resulting from step (c) to yield a side group capable of covalent bonding with reactive groups of a hydrophobic moiety, (e) reacting the product of step (d) with a hydrophobic moiety to yield the modified antigen of claim 3.
Claim 13
Dependent Claim
38 words
The method of claim 11 wherein step (d) comprises subjecting the O-antigenic polysaccharide to periodate oxidation to yield aldehyde to periodate space oxidation to yield aldehyde groups capable of covalent bonding with amino groups of a hydrophobic variety.
References Cited (Backward Citations)
US4264766A
Cited
US3148120A
Cited
US3342684A
Cited
US4029762A
Cited
US4402939A
Cited
US4397959A
Cited
US4356170A
Cited
SU988865A1
Cited
US4652518A
Cited
US4603197A
Cited
JPH05112921A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-15 | 1992-11-14 | 1993-05-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-15 | 1996-11-14 | 1997-05-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-15 | 2000-11-14 | 2001-05-16 | $7700.00 | Window Closed / Lapsed |