Brucellosis antigens

Claim 1

A SUBSTANTIALLY NON-TOXIC AND NON-AGGLUTINOGENIC IMMONOGENIC SUBSTANCE, IDENTIFIED AS ANTIGEN A12, WHICH IS USEFUL IN CONFERRING IMMUNITY AGAINST BRUCELLOSIS, IN A PHYSIOLOGICALLY ACCEPTABLE INJECTABLE CARRIER, SAID IMMONOGENIC SUBSTANCE BEING A SUBSTANTIALLY PURE GLYCOLIPOPROTEIN CHARACTERIZED BY THE FOLLOWING PROPERTIES: SOLUBLE IN WATER (> 1 G./100 ML.); SUBSTANTIALLY FREE OF NUCLEIC ACIDS; POSITIVE RESPONSES TO THE MOLISCH REACTION OF CARBOHYDRATES, XANTHOPROTEIN REACTION FOR AMINO ACIDS CONTAINING AROMATIC STRUCTURES, AND BIURET REACTION FOR PROTEINS AND POLYPEPTIDES; STRONGLY POSITIVE RESPONSE FOR LIPID CONTENT; CONTAINING ABOUT 41-46% "GLUCOSE UNITS" AND CHROMATOGRAPHICALLY SHOWING APPROXIMATE SUGAR CONTENTS OF ABOUT 10% GALACTOSE, ABOUT 2% GLUCOSE, ABOUT 1.5% MANNOSE, ABOUT 1% XYLOSE, AN UNIDENTIFIABLE FAST-RUNING COMPONENT, AND A POSITIVE RESPONSE FOR AMINO-SUGARS; OPTICAL ROTATION (1.14% IN DISTILLED WATER) OF ($)20(**D)= +12(+ OR -1.2 DEG) AN ELEMENTAL ANALYSIS OF ABOUT 30.5-34.5% CARBON, ABOUT 5.0-5.5% HYDROGEN, ABOUT 2.0-4.0% NITROGEN, ABOUT 1.02.0% PHOSPHORUS, ABOUT 4.5-6.0% SODIUM, ABOUT 2.04.0% H2O AND ABOUT 6.0-8.0% VOLATILES; SHOWS ONLY A WEAK MAXIMUM OF ABSORPTION AT 258 MU IN THE ULTRAVIOLET REGION OF THE SPECTRUM, AND IN THE INFRARED REGION EXHIBITS NO CHARACTERISTIC SHARP BANDS; A SEDIMENTATION CONSTANT (1% SOLUTION IN 1/15 M PHOSPHATE, PH 7.0 OF S20=1.41.5S; INSIGNIFICANT AGGLUTINATION TITRES IN RABBITS AT 5-80 MICROGRAMS/KG. BODY WEIGHT; AND AN LD50 IN MICE >200 MG./KG. BODY WEIGHT.