US3148120A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Hot aqueous phenol extraction of gramnegative bacterial lipopolysaccharides
Assignee: Wander AG
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Filing: 1958-11-07
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Granted: 1964-09-08
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Expires: 1978-11-07
Abstract
No abstract record available.
Inventors
- 👤 Westphal Otto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 14:54
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/04
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
96 words
IN A PROCESS OF PREPARING SUBSTANTIALLY PROTEIN-FREE LIPOPOLYSACCHARIDES FROM GRAM-NEGATIVE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY, THE STEPS, COMPRISING TREATING SAID BACTERIA WITH A PHENOL-WATER MIXTURE CONTAINING BETWEEN ABOUT 37.0% AND ABOUT 50.0% OF PHENOL AT A TEMPERATURE BETWEEN ABOUT 60*C. AND ABOUT 70*C, THEREBY CAUSING DISSOCIATION OF THE LIPOPOLYSACCHARIDE-PROTEIN COMPLEX COMPOUND PRESENT IN SAID BACTERIA, COOLING THE RESULTING MIXTURE TO A TEMPERATURE OF LESS THAN ABOUT 40* C. TO CAUSE SEPARTION OF SAID MIXTURE INTO AN AQUEOUS LAYER AND A PHENOLIC LAYER, REMOVING SAID AQUEOUS LAYER, AND ISOLATING FROM SAID AQUEOUS LAYER THE SUBSTANTIALLY PROTEIN-FREE LIPOPOLYSACCHARIDE.
Claim 2
Dependent Claim
96 words
IN A PROCESS OF PREPARING SUBSTANTIALLY PROTEIN-FREE LIPOPOLYSACCHARIDES FROM GRAM-NEGATIVE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY, THE STEPS, COMPRISING TREATING SAID BACTERIA WITH A PHENOL-WATER MIXTURE CONTAINING BETWEEN ABOUT 37.0% AND ABOUT 50.0% OF PHENOL AT A TEMPERATURE BETWEEN ABOUT 60*C. AND ABOUT 70*C, THEREBY CAUSING DISSOCIATION OF THE LIPOPOLYSACCHARIDE-PROTEIN COMPLEX COMPOUND PRESENT IN SAID BACTERIA, COOLING THE RESULTING MIXTURE TO A TEMPERATURE OF LESS THAN ABOUT 40* C. TO CAUSE SEPARTION OF SAID MIXTURE INTO AN AQUEOUS LAYER AND A PHENOLIC LAYER, REMOVING SAID AQUEOUS LAYER, AND ISOLATING FROM SAID AQUEOUS LAYER THE SUBSTANTIALLY PROTEIN-FREE LIPOPOLYSACCHARIDE.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1967-09-08 | 1968-03-09 | 1968-09-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1971-09-08 | 1972-03-09 | 1972-09-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1975-09-08 | 1976-03-09 | 1976-09-08 | $7700.00 | Window Closed / Lapsed |