US4397959A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Forced precipitation method for preparing antigen/antibody particles
Assignee: RESEARCH Corp A NEW YORK NOT-FOR-PROFIT CORP
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Filing: 1980-11-05
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Granted: 1983-08-09
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Expires: 2000-11-05
Abstract
Abstract Process for the preparation of test reagents comprising antigens or antibodies adsorbed on a surface, for example, the surface of synthetic or natural polymer particles in which the test material to be adsorbed is dissolved in a solvent in contact with the adsorbing surface and precipitated by the addition of a liquid which is miscible with the solvent, but does not dissolve the test material.
Inventors
- 👤 Karim E. Hechemy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 08:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56911; G01N33/543; G01N33/54393; G01N33/544; G01N33/564; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S436/00; Y10S436/823; Y10S436/826
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
102 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of antigens or antibodies, said reagent comprising a test material of antigen or antibody adsorbed on the adsorbent surface of an adsorbent substrate which comprises preparing a solution containing dissolved test material in a solvent in the presence of an adsorbent substrate and adding to the solution an organic liquid precipitating reagent which is miscible with the solvent but does not dissolve the test material or the adsorbent substrate whereby the test material precipitates as an adsorbent on the surface of the adsorbent substrate.
Claim 2
Dependent Claim
102 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of antigens or antibodies, said reagent comprising a test material of antigen or antibody adsorbed on the adsorbent surface of an adsorbent substrate which comprises preparing a solution containing dissolved test material in a solvent in the presence of an adsorbent substrate and adding to the solution an organic liquid precipitating reagent which is miscible with the solvent but does not dissolve the test material or the adsorbent substrate whereby the test material precipitates as an adsorbent on the surface of the adsorbent substrate.
Claim 3
Dependent Claim
94 words
A process for the preparation of test reagents for serological testing of mammalian sera for the presence of antigens or antibodies said reagent comprising a test material of antigen or antibody adsorbed on the adsorbent surface of an adsorbent substrate which comprises preparing in a container an aqueous solution said solution containing dissolved test material in the presence of the adsorbent substrate and adding to the solution a mixture of a water soluble alkali metal salt and a lower water soluble alcohol whereby the test material precipitates as an adsorbent on the adsorbent surface.
Claim 4
Dependent Claim
94 words
A process for the preparation of test reagents for serological testing of mammalian sera for the presence of antigens or antibodies said reagent comprising a test material of antigen or antibody adsorbed on the adsorbent surface of an adsorbent substrate which comprises preparing in a container an aqueous solution said solution containing dissolved test material in the presence of the adsorbent substrate and adding to the solution a mixture of a water soluble alkali metal salt and a lower water soluble alcohol whereby the test material precipitates as an adsorbent on the adsorbent surface.
Claim 5
Dependent Claim
16 words
A process as in claim 2 wherein the adsorbent surface is the surface of a particle.
Claim 6
Dependent Claim
18 words
A process as in claim 2 wherein the adsorbent surface is the substantially flat surface of the container.
Claim 7
Dependent Claim
35 words
A process as in claim 2 wherein the adsorbent surface is the substantially flat surface of a polymer membrane supported in the aqueous solution when the mixture of alkali metal salt and alcohol is added.
Claim 8
Dependent Claim
65 words
A process for the preparation of tests reagents suitable for serological testing of mammalian sera for the presence of a rickettsial infection which comprises preparing an aqueous solution containing a dissolved rickettsia antigen and suspended adsorbent particles and adding to the solution a mixture of a water soluble alkali metal salt and a lower water soluble alcohol whereby the antigen precipitates in the adsorbent surface.
Claim 9
Dependent Claim
65 words
A process for the preparation of tests reagents suitable for serological testing of mammalian sera for the presence of a rickettsial infection which comprises preparing an aqueous solution containing a dissolved rickettsia antigen and suspended adsorbent particles and adding to the solution a mixture of a water soluble alkali metal salt and a lower water soluble alcohol whereby the antigen precipitates in the adsorbent surface.
Claim 10
Dependent Claim
12 words
A process as in claim 6 wherein the adsorbent is a latex.
Claim 11
Dependent Claim
24 words
A process as in claim 7 wherein the rickettsia is Rickettsia rickettsii; the alkali metal salt is sodium acetate and the alcohol is ethanol.
Claim 12
Dependent Claim
24 words
A process as in claim 7 wherein the rickettsia is Rickettsia prowazeki, the alkali metal salt is sodium acetate and the alcohol is ethanol.
Claim 13
Dependent Claim
24 words
A process as in claim 7 wherein the rickettsia is Rickettsia typhi, the alkali metal salt is sodium acetate and the alcohol is ethanol.
Claim 14
Dependent Claim
68 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of rheumatoid arthritis antibodies which comprises preparing solution of Fraction II globulin containing a suspension of latex particles and adding to the solution a mixture of water soluble alkali metal salt and a water soluble alcohol whereby the Fraction II globulin precipitates on the adsorbent surface of the latex particles.
Claim 15
Dependent Claim
68 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of rheumatoid arthritis antibodies which comprises preparing solution of Fraction II globulin containing a suspension of latex particles and adding to the solution a mixture of water soluble alkali metal salt and a water soluble alcohol whereby the Fraction II globulin precipitates on the adsorbent surface of the latex particles.
Claim 16
Dependent Claim
17 words
A process as in claim 11 wherein the salt is sodium acetate and the alcohol is ethanol.
Claim 17
Dependent Claim
71 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of Escherichia coli antibodies which comprises preparing a solution of dissolved lipopolysaccharide antigens from Escherichia coli containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
Claim 18
Dependent Claim
71 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of Escherichia coli antibodies which comprises preparing a solution of dissolved lipopolysaccharide antigens from Escherichia coli containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
Claim 19
Dependent Claim
17 words
A process as in claim 13 wherein the salt is sodium acetate and the aclohol is ethanol.
Claim 20
Dependent Claim
67 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of trichinosis antibodies which comprises preparing a solution of dissolved trichinosis antigen containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
Claim 21
Dependent Claim
67 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of trichinosis antibodies which comprises preparing a solution of dissolved trichinosis antigen containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
Claim 22
Dependent Claim
17 words
A process as in claim 15 wherein the salt is sodium acetate and the alcohol is ethanol.
Claim 23
Dependent Claim
67 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of toxoplasma antibodies which comprises preparing a solution of dissolved toxoplasma antigen containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
Claim 24
Dependent Claim
67 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of toxoplasma antibodies which comprises preparing a solution of dissolved toxoplasma antigen containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
Claim 25
Dependent Claim
17 words
A process as in claim 17 wherein the salt is sodium acetate and the alcohol is ethanol.
Claim 26
Dependent Claim
67 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of chlamydial antibodies which comprises preparing a solution of dissolved chlamydial antigen containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
Claim 27
Dependent Claim
67 words
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of chlamydial antibodies which comprises preparing a solution of dissolved chlamydial antigen containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
Claim 28
Dependent Claim
17 words
A process as in claim 19 wherein the salt is sodium acetate and the alcohol is ethanol.
References Cited (Backward Citations)
US3708572A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-08-08 | 1987-02-07 | 1987-08-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-08-08 | 1991-02-07 | 1991-08-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-08-08 | 1995-02-07 | 1995-08-09 | $7700.00 | Window Closed / Lapsed |