US4721673A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Recovery and activation process for microbially produced calf prochymosin
Assignee: Genex Corp
•
Filing: 1984-03-06
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Granted: 1988-01-26
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Expires: 2004-03-06
Abstract
Abstract A process for the extraction and activation of microbially produced bovine prochymosin is disclosed.
Inventors
- 👤 Jack R. Uren
- 👤 Douglas E. Robinson
- 👤 Carl J. Scandella
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 02:27
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; C07; C07K; C07K1/00; C07K1/107; C07K1/113; C07K1/1136; C12N9/52; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816; Y10S930/00; Y10S930/01; Y10S930/31
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
105 words
A process for converting microbially produced bovine prochymosin into a renatured form of prochymosin which is capable of conversion to pseudochymosin at a pH of about 2.0, which process comprises: (a) solubilizing a preparation of microbially produced bovine prochymosin in a solution of a base, said solution having a pH of at least 12.0; (b) reducing the pH of said solution to between 8.5 and 10.5; and (c) maintaining said preparation of microbially produced bovine prochymosin in said solution, thereby converting said microbially produced bovine prochymosin into a renatured form of prochymosin which is capable of conversion to pseudochymosin at a pH of about 2.0.
Claim 2
Dependent Claim
105 words
A process for converting microbially produced bovine prochymosin into a renatured form of prochymosin which is capable of conversion to pseudochymosin at a pH of about 2.0, which process comprises: (a) solubilizing a preparation of microbially produced bovine prochymosin in a solution of a base, said solution having a pH of at least 12.0; (b) reducing the pH of said solution to between 8.5 and 10.5; and (c) maintaining said preparation of microbially produced bovine prochymosin in said solution, thereby converting said microbially produced bovine prochymosin into a renatured form of prochymosin which is capable of conversion to pseudochymosin at a pH of about 2.0.
Claim 3
Dependent Claim
10 words
Th process of claim 1, wherein the base is NaOH.
Claim 4
Dependent Claim
24 words
The process of claim 1, wherein the prochymosin is solubilized in a sodium hydroxide solution having a sodium hydroxide concentration of at least 0.01M.
Claim 5
Dependent Claim
109 words
A process for converting microbially produced bovine prochymosin into pseudochymosin, which process comprises: (a) solubilizing a preparation of microbially produced bovine prochymosin in a solution of a base, said solution having a pH of at least 12.0; (b) reducing the pH of said solution to between 8.5 and 10.5; (c) maintaining said preparation of microbially produced bovine prochymosin in said solution, thereby converting said preparation of microbially produced bovine prochymosin into a renatured form of prochymosin which is capable of conversion of pseudochymosin at a pH of about 2.0; and (d) reducing the pH of said solution to below 2.5, thereby converting said microbially produced bovine prochymosin into pseudochymosin.
Claim 6
Dependent Claim
109 words
A process for converting microbially produced bovine prochymosin into pseudochymosin, which process comprises: (a) solubilizing a preparation of microbially produced bovine prochymosin in a solution of a base, said solution having a pH of at least 12.0; (b) reducing the pH of said solution to between 8.5 and 10.5; (c) maintaining said preparation of microbially produced bovine prochymosin in said solution, thereby converting said preparation of microbially produced bovine prochymosin into a renatured form of prochymosin which is capable of conversion of pseudochymosin at a pH of about 2.0; and (d) reducing the pH of said solution to below 2.5, thereby converting said microbially produced bovine prochymosin into pseudochymosin.
Claim 7
Dependent Claim
10 words
The process of claim 4 wherein the base is NaOH.
Claim 8
Dependent Claim
24 words
The process of claim 4, wherein the prochymosin is solubilized in a sodium hydroxide solution having a sodium hydroxide concentration of at least 0.01M.
Claim 9
Dependent Claim
18 words
The process of claim 4, wherein the pH of the renatured prochymosin solution is reduced to about 2.0.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-01-25 | 1991-07-27 | 1992-01-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-01-25 | 1995-07-27 | 1996-01-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-01-25 | 1999-07-27 | 2000-01-26 | $7700.00 | Window Closed / Lapsed |