US4512922A
Expired / Lapsed
US
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Purification and activity assurance of precipitated heterologous proteins
Assignee: Genentech Inc
•
Filing: 1984-06-01
•
Granted: 1985-04-23
•
Expires: 2004-06-01
Abstract
Abstract A process for maintaining refractile proteins in solubilized form by replacing the strongly denaturing solution with a weakly denaturing solution.
Inventors
- 👤 Andrew J. S. Jones
- 👤 Kenneth C. Olson
- 👤 Steven J. Shire
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 13:04
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6478; C12N9/6481; C07; C07K; C07K1/00; C07K1/107; C07K1/113; C07K1/1133; C12N9/6424; C12N9/6456; C12N9/6462; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S530/00; Y10S530/82; Y10S530/825; Y10S530/826
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
49 words
A process for solubilizing and maintaining the solubility of protein precipitated in the form of refractile bodies in a host cell culture, comprising solubilizing said refractile bodies in a strongly denaturing solution, and replacing the strongly denaturing solution with a different weakly denaturing medium while keeping the protein solubilized.
Claim 2
Dependent Claim
49 words
A process for solubilizing and maintaining the solubility of protein precipitated in the form of refractile bodies in a host cell culture, comprising solubilizing said refractile bodies in a strongly denaturing solution, and replacing the strongly denaturing solution with a different weakly denaturing medium while keeping the protein solubilized.
Claim 3
Dependent Claim
19 words
The process of claim 1 wherein the strongly denaturing solution is 4 to 9M in a salt of guanidine.
Claim 4
Dependent Claim
17 words
The process of claim 1 wherein the weakly denaturing solution is approximately 1 to 9M in urea.
Claim 5
Dependent Claim
25 words
The process of claim 1 which includes at least one step performed using the weakly denaturing solution of the protein, designed to purify the protein.
Claim 6
Dependent Claim
8 words
Protein prepared by the process of claim 1.
Claim 7
Dependent Claim
19 words
The process of claim 1 wherein buffer exchange replaces said strongly denaturing solution with said different weakly denaturing solution.
Claim 8
Dependent Claim
13 words
The process of claim 6 wherein said buffer exchange uses dialysis or diafiltration.
Claim 9
Dependent Claim
18 words
The process of claim 6, wherein said buffer exchange is conducted in the presence of a reducing agent.
Claim 10
Dependent Claim
11 words
The process of claim 8, wherein said reducing agent is β-mercaptoethanol.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-22 | 1988-10-22 | 1989-04-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-22 | 1992-10-22 | 1993-04-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-22 | 1996-10-22 | 1997-04-23 | $7700.00 | Window Closed / Lapsed |