EP0077670A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Human immune interferon
Assignee: Genentech Inc
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Filing: 1982-10-18
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Granted: —
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Expires: 2002-10-18
Abstract
Abstract Disclosed is a complete description of the preparation of a novel, substantially pure polypeptide via recombinant DNA techniques utilizing any of an assortment of expression vectors and host cultures. The polypeptide, human immune (gamma) interferon (IFN-y), is isolated in substantially pure form and characterized in terms of DNA and amino acid sequences (see Figure 5), physical attributes and biological activity.
Inventors
- 👤 David V. Goeddel
- 👤 Patrick W. Gray
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 09:44
CPC Classifications
C; C12; C12P; C12P21/00; C12P21/02; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; A; A61; A61K; A61K38/00; A61K38/16; A61K38/17; A61K38/19; A61K38/21; A61P; A61P31/00; A61P31/12; A61P35/00; A61P37/00; A61P37/02; A61P37/06; A61P43/00; C07K14/57; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/71; C12N15/79; C12N15/80; C12N15/81; C12N15/85; Y; Y10; Y10S; Y10S435/00; Y10S435/811; Y10S930/00; Y10S930/01; Y10S930/14; Y10S930/142; Y10S930/30
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
19 words
A composition of matter consisting essentially of a polypeptide comprising the amino acid sequence of mature human immune interferon.
Claim 2
Dependent Claim
19 words
A composition of matter consisting essentially of a polypeptide comprising the amino acid sequence of mature human immune interferon.
Claim 3
Dependent Claim
11 words
The polypeptide according to Claim 1, unaccompanied by associated native glycosylation.
Claim 4
Dependent Claim
25 words
The polypeptide according to Claim 1, optionally containing the amino acid methionine attached to the N-terminus of the ordinarily first amino acid of said interferon.
Claim 5
Dependent Claim
28 words
The polypeptide according to Claim 1, optionally . containing a cleavable conjugate or signal protein attached to the N-terminus of the ordinarily first amino acid of said interferon.
Claim 6
Dependent Claim
17 words
A DNA sequence comprising a sequence coding of the polypeptide according to Claims 1, 3 or 4.
Claim 7
Dependent Claim
27 words
The DNA sequence according to Claim 5 operably linked with a DNA sequence capable of effecting expression of a polypeptide according to Claims: 1, 3 or 4.
Claim 8
Dependent Claim
23 words
A replicable expression vehicle capable, in a transformant microorganism or cell culture, of expressing a polypeptide according to Claims 1, 3 or 4.
Claim 9
Dependent Claim
13 words
A microorganism or cell culture transformed with the vehicle according to Claim 7.
Claim 10
Dependent Claim
16 words
The cell culture according to Claim 8, obtained by transforming COS-7 line of monkey kidney fibroblasts.
Claim 11
Dependent Claim
13 words
A microorganism according to Claim 8, obtained by transforming an E. coli strain.
Claim 12
Dependent Claim
12 words
A microorganism according to Claim 8, obtained by transforming a yeast strain.
Claim 13
Dependent Claim
13 words
A plasmid selected from the group consisting of pIFN-y-trp 48, pSV-γ-69, and pPGK-IFN-γ.
Claim 14
Dependent Claim
13 words
A plasmid selected from the group consisting of pIFN-y-trp 48, pSV-γ-69, and pPGK-IFN-γ.
Claim 15
Dependent Claim
15 words
A microorganism or cell culture transformed with each of the plasmids according to Claim 12.
Claim 16
Dependent Claim
24 words
A composition of matter comprising a therapeutically active fraction of a polypeptide consisting essentially of the amino acid sequence of mature human immune interferon.
Claim 17
Dependent Claim
24 words
A composition of matter comprising a therapeutically active fraction of a polypeptide consisting essentially of the amino acid sequence of mature human immune interferon.
Claim 18
Dependent Claim
31 words
A cell culture extact comprising greater than about 90 percent pure polypeptide consisting essentially of the amino acid sequence of mature immune interferon according to Claims 1, 2, 3, or 4.
Claim 19
Dependent Claim
23 words
A composition comprising a therapeutically effective amount of a mature human immune-interferon according to Claims 1, 3 or 4, suitable for pharmaceutical administration.
Claim 20
Dependent Claim
10 words
The composition according to Claim 16 suitable for parenteral administration.
Claim 21
Dependent Claim
14 words
A culture of transformant cells capable of producing human immune interferon in mature form.
Claim 22
Dependent Claim
14 words
A culture of transformant cells capable of producing human immune interferon in mature form.
Claim 23
Dependent Claim
32 words
19 . The use of a mature human immune interferon according to Claims 1, 3 or 4, for antitumor, antiviral or immunosuppression treatment or for preparing pharmaceutical compositions useful for such treatment.
Claim 24
Dependent Claim
20 words
A process which comprises expressing a gene encoding human immune interferon in mature form in a microorganism or cell culture.
Claim 25
Dependent Claim
20 words
A process which comprises expressing a gene encoding human immune interferon in mature form in a microorganism or cell culture.
Claim 26
Dependent Claim
20 words
A process which comprises producing a polypeptide according to Claims 1, 3 or 4 in a microorganism or cell culture.
Claim 27
Dependent Claim
41 words
A process for producing a polypeptide according to Claim 1, 3 or 4 comprising causing a culture of a microrganism or cell culture, transformed with a replicable expression vehicle, to grow and effect production of said polypeptide and recovering said polypeptide.
Claim 28
Dependent Claim
46 words
A process for producing an expression vehicle according to Claim 7 comprising constructing a first DNA sequence coding for said polypeptide (defined in Claim 7) and operably linking said first DNA sequence with a second DNA sequence capable of effecting expression of said first DNA sequence.
Claim 29
Dependent Claim
13 words
A product of the process according to Claims 20, 21, 22 or 23.
Claim 30
Dependent Claim
17 words
A composition of matter comprising mature human immune interferon essentially free of other proteins of human origin.
Claim 31
Dependent Claim
17 words
A composition of matter comprising mature human immune interferon essentially free of other proteins of human origin.
Claim 32
Dependent Claim
17 words
A substantially pure polypeptide whose amino acid sequence is substantially as is shown in Figure 5 hereof.
Claim 33
Dependent Claim
17 words
A substantially pure polypeptide whose amino acid sequence is substantially as is shown in Figure 5 hereof.
References Cited (Backward Citations)
No citation records indexed.
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