EP0068693A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Production of foot and mouth disease vaccine from microbially expressed antigens
Assignee: Genentech Inc
•
Filing: 1982-06-11
•
Granted: —
•
Expires: 2002-06-11
Abstract
Abstract Polypeptides which act as antigens of foot and mouth disease virus (FMDV) are produced by microorganisms which have been transformed with plasmids produced using recombinant DNA technology. A suitable plasmid has a gene sequence (produced by chemical synthesis or by reverse transcription) which encodes a polypeptide comprising one or more immunogenic determinants of one or more FMDV proteins, and optionally a fusion protein preferably linked via a cleavage site, the gene sequence being under the control of a promoter. For example, an E. Coli K12 strain was transformed with plasmid pFM 1 . This is based on plasmid pBR322, with the DNA sequence of codons 8 to 210 of the VP, gene of FMDV type A 12 119ab connected to the LE' protein (from pBR322) so as to be in the same reading frame. This assembly is under the control of the E. Coli trp operon. The transformant E. Coli was cultured and caused to express the LE'-VP, fusion protein. This was isolated and shown by competitive radio immunoassay to have similar antigenic properties to purified natural VP, protein.
Inventors
- 👤 Dennis G. Kleid
- 👤 Daniel G. Yansura
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 18:41
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; C12; C12N; C12N2770/00; C12N2770/00011; C12N2770/32011; C12N2770/32111; C12N2770/32122
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
24 words
An inmunogenicany active, microbially produced polypeptide comprising the amino acid sequence of at least one antigenic determinant of foot and mouth disease virus protein.
Claim 2
Dependent Claim
24 words
An inmunogenicany active, microbially produced polypeptide comprising the amino acid sequence of at least one antigenic determinant of foot and mouth disease virus protein.
Claim 3
Dependent Claim
35 words
An immunogenically active, microbially produced polypeptide comprising the amino acid sequence of at least two antigenic determinants of foot and mouth disease virus protein from at least two different foot and mouth disease virus strains.
Claim 4
Dependent Claim
35 words
An immunogenically active, microbially produced polypeptide comprising the amino acid sequence of at least two antigenic determinants of foot and mouth disease virus protein from at least two different foot and mouth disease virus strains.
Claim 5
Dependent Claim
28 words
The polypeptide according to Claim 2 wherein said strains are selected from the group consisting of A, C, 0, Asia-1, SAT 1, SAT 2 and SAT 3 types.
Claim 6
Dependent Claim
19 words
The polypeptide according to Claim 1, 2 or 3 fused with additional polypeptide derived from expression of additional DNA.
Claim 7
Dependent Claim
31 words
The polypeptide according to Claim 4 wherein said additional DNA is synthetically derived and is in functional reading phase with DNA of the polypeptide of foot and mouth disease virus protein.
Claim 8
Dependent Claim
37 words
The polypeptide according to Claim 4 wherein said additional DNA is synthetically derived and is in functional reading phase with DNA encoding microbial polypeptide and with DNA of the polypeptide of foot and mouth disease virus protein.
Claim 9
Dependent Claim
18 words
The polypeptide according to Claim 4, 5 or 6, wherein said additional DNA encodes ATG in reading phase
Claim 10
Dependent Claim
12 words
The polypeptide according to any of Claims 1 to 7, directly expressed.
Claim 11
Dependent Claim
23 words
The polypeptide according to any preceding Claim comprising amino acids 55 to 177 of the VP 3 foot and mouth disease virus protein.
Claim 12
Dependent Claim
23 words
The polypeptide according to any preceding Claim comprising amino acids 1 to 210 of the VP 3 foot and mouth disease virus protein.
Claim 13
Dependent Claim
22 words
The polypeptide according to Claim 4 comprising amino acids 7 to 210 of the VP 3 foot and mouth disease virus protein.
Claim 14
Dependent Claim
39 words
The polypeptide according to Claim 2 comprising amino acids 1 to 210 of the VP 3 foot and mouth disease virus protein fused to amino acids 8 to 210 of the VP 3 foot and mouth disease virus protein.
Claim 15
Dependent Claim
15 words
A DNA sequence comprising a sequence coding for a polypeptide according to any preceding Claim.
Claim 16
Dependent Claim
22 words
A replicable microbial expression vehicle capable, in a transformant microorganism, of expressing a polypeptide according to any of Claims 1 to 12.
Claim 17
Dependent Claim
13 words
A replicable microbial expression vehicle comprising a DNA sequence according to Claim 13.
Claim 18
Dependent Claim
23 words
A plasmid selected from pFM,, pFM 2 , pFM 3 , pFM 10 , pFM 20 , pFMB1, pFMC, pFMD, pFMF and pFMG.
Claim 19
Dependent Claim
23 words
A plasmid selected from pFM,, pFM 2 , pFM 3 , pFM 10 , pFM 20 , pFMB1, pFMC, pFMD, pFMF and pFMG.
Claim 20
Dependent Claim
14 words
A microorganism transformed with a vehicle according to any of Claims 14 to 16.
Claim 21
Dependent Claim
13 words
A microorganism according to Claim 17, which is a transformed E. coli strain.
Claim 22
Dependent Claim
13 words
A microbial culture comprising a transformant microorganism according to Claim 17 or 18.
Claim 23
Dependent Claim
16 words
A microbial culture capable of producing a polypeptide according to any of Claims 1 to 12.
Claim 24
Dependent Claim
32 words
The use of a polypeptide according to any of Claims 1 to 12 for conferring immunogenicity to foot and mouth disease in a susceptible animal or for preparing a vaccine useful therefor.
Claim 25
Dependent Claim
31 words
A vaccine for use in conferring immunogenicity to foot and mouth disease in a susceptible animal comprising a polypeptide according to any of Claims 1 to 12 and a suitable vehicle.
Claim 26
Dependent Claim
20 words
A vaccine against foot and mouth disease comprising an immunogenically active, microbially produced constituent of foot and mouth disease virus.
Claim 27
Dependent Claim
20 words
A vaccine against foot and mouth disease comprising an immunogenically active, microbially produced constituent of foot and mouth disease virus.
Claim 28
Dependent Claim
29 words
A vaccine according to Claim 23 wherein said constituent is a polypeptide comprising the amino acid sequence of at least one antigenic determinant of foot and mouth disease virus.
Claim 29
Dependent Claim
19 words
A vaccine according to Claim 24 comprising antigenic determinant(s) of one or more foot and mouth disease virus strains.
Claim 30
Dependent Claim
32 words
A vaccine according to Claim 23, 24 or 25 wherein the foot and mouth disease virus strain is selected from A, C, 0, Asia-1, SAT 1, SAT 2, and SAT 3 types.
Claim 31
Dependent Claim
26 words
A process which comprises microbially producing a polypeptide comprising the amino acid sequence of at least one antigenic determinant of foot and mouth disease virus protein.
Claim 32
Dependent Claim
26 words
A process which comprises microbially producing a polypeptide comprising the amino acid sequence of at least one antigenic determinant of foot and mouth disease virus protein.
Claim 33
Dependent Claim
61 words
The use of a DNA sequence according to Claim 13 or of an expression vehicle according to any of Claims 14 to 16 or of a microorganism or culture according to any of Claims 17 to 20 in the preparation of a polypeptide comprising the amino acid sequence of at least one antigenic determinant of foot and mouth disease virus protein.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.