US3616233A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Removing esterase from microbial rennin
Assignee: Baxter International Inc
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Filing: 1969-02-19
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Granted: 1971-10-26
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Expires: 1989-02-19
Abstract
Abstract Esterase is removed from microbial rennin by acidifying the rennin to a pH of about 2.0 to 3.5 and maintaining the rennin at a temperature of about 20* C. to 55* C. until the esterase is decomposed. Thereafter, the rennin is preferably neutralized to a pH of at least about 4 to inhibit its decomposition.
Inventors
- 👤 Hans Schleich
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 17:08
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/58; C12N9/16; C12N9/18; C12N9/20; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/834
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
19 words
The process of claim 1 in which said rennin is separated from said micro-organism culture prior to said acidifying.
Claim 2
Dependent Claim
31 words
The process of claim 1 in which said microbial rennin is neutralized to a pH of at least about 4 after said esterase is destroyed, to inhibit decomposition of said rennin.
Claim 3
Dependent Claim
34 words
The process of claim 1 in which said rennin is maintained at a ph of 2.0 to 3.5 and a temperature of 20* to 55* C. for a period of 1/2 to 5 hours.
Claim 4
Dependent Claim
34 words
The process of claim 4 in which said rennin is maintained at a pH of 2.5 to 3 and a temperature between 40* to 50* C. for a period of 1 to 5 hours.
Claim 5
Dependent Claim
34 words
The process of claim 4 in which said rennin is maintained at a pH of 2.3 to 2.5 and a temperature of 25* to 40* C. for a period of 1 to 5 hours.
Claim 6
Dependent Claim
16 words
The process of claim 1 in which said rennin is prepared from the organism Mucor miehei.
Claim 7
Dependent Claim
84 words
The process of removing an esterase from a microbial rennin previously prepared by aerobically fermenting a nutrient wiTh a culture of the organism Mucor miehei to produce a rennin mixed with an esterase at a pH of at least about 4, which comprises acidifying the rennin and esterase thereby produced to a pH of 2.0 to 3.5 and maintaining said rennin at a temperature of 20* to 55* C. for a period of time sufficient to destroy a substantial portion of the esterase present.
Claim 8
Dependent Claim
18 words
The process of claim 8 in which said rennin is separated from said culture prior to said acidifying.
Claim 9
Dependent Claim
31 words
The process of claim 8 in which said rennin is neutralized to provide a pH of at least about 4 after said esterase is destroyed, to inhibit decomposition of said rennin.
Claim 10
Dependent Claim
34 words
The process of claim 8 in which said rennin is maintained at a pH of 2.0 to 3.5 and a temperature of 20* to 55* C. for a period of 1/2 to 5 hours.
Claim 11
Dependent Claim
34 words
The process of claim 11 in which said rennin is maintained at a pH of 2.5 to 3 and a temperature of 40* to 50* C. for a period of 1 to 5 hours.
Claim 12
Dependent Claim
34 words
The process of claim 11 in which said rennin is maintained at a pH of 2.3 to 2.5 and a temperature of 25* to 40* C. for a period of 1 to 5 hours.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-10-25 | 1975-04-26 | 1975-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-10-25 | 1979-04-26 | 1979-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-10-25 | 1983-04-26 | 1983-10-26 | $7700.00 | Window Closed / Lapsed |